Cytokine Factors Present in Dengue Patient Sera Induces Alterations of Junctional Proteins in Human Endothelial Cells

Ramapraba Appanna Department of Medical Microbiology, Department of Medicine, and Department of Pediatrics, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; Institute of Medical Molecular Biotechnology, Faculty of Medicine, Universiti Teknologi MARA, Selangor, Malaysia

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Seok Mui Wang Department of Medical Microbiology, Department of Medicine, and Department of Pediatrics, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; Institute of Medical Molecular Biotechnology, Faculty of Medicine, Universiti Teknologi MARA, Selangor, Malaysia

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Sasheela A. Ponnampalavanar Department of Medical Microbiology, Department of Medicine, and Department of Pediatrics, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; Institute of Medical Molecular Biotechnology, Faculty of Medicine, Universiti Teknologi MARA, Selangor, Malaysia

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Lucy Chai See Lum Department of Medical Microbiology, Department of Medicine, and Department of Pediatrics, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; Institute of Medical Molecular Biotechnology, Faculty of Medicine, Universiti Teknologi MARA, Selangor, Malaysia

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Shamala Devi Sekaran Department of Medical Microbiology, Department of Medicine, and Department of Pediatrics, Faculty of Medicine, University of Malaya, Kuala Lumpur, Malaysia; Institute of Medical Molecular Biotechnology, Faculty of Medicine, Universiti Teknologi MARA, Selangor, Malaysia

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DOI:
https://doi.org/10.4269/ajtmh.2012.11-0606
Volume/Issue:
Volume 87: Issue 5
Page(s):
936–942
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Plasma leakage in severe dengue has been postulated to be associated with skewed cytokine immune responses. In this study, the association of cytokines with vascular permeability in dengue patients was investigated. Human serum samples collected from 48 persons (13 with dengue fever, 29 with dengue hemorrhagic fever, and 6 healthy) were subjected to cytokines analysis by using Luminex Multiplex Technology. Selected serum samples from patients with dengue hemorrhagic fever sera and recombinant human cytokines were then tested for roles on inducing vascular permeability by treatment of human umbilical vein endothelial cells. Confocal immunofluorescence staining indicated morphologic alteration of human umbilical vein endothelial cells treated with serum samples from patients with dengue hemorrhagic fever compared with serum samples from healthy persons. The findings suggest that cytokines produced during dengue hemorrhagic infections could induce alterations in the vascular endothelium, which may play a fundamental role in the pathophysiology of dengue.

Author Notes

* Address correspondence to Shamala Devi Sekaran, Department of Medical Microbiology, Faculty of Medicine, University of Malaya, 50603 Kuala Lumpur, Malaysia. E-mail: shamalamy@yahoo.com

Financial support: This study was supported by University of Malaya research grants UMRG RG081-09HTM and HIRG J-00000-73560.

Disclosure: None of the authors have any conflicts of interest.

Authors' addresses: Ramapraba Appanna and Shamala Devi Sekaran, Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia, E-mails: ag_praba@yahoo.com and shamalaya@yahoo.com. Seok Mui Wang, Institute of Medical Molecular Biotechnology, Faculty of Medicine, Universiti Teknologi MARA, Selangor, Malaysia, E-mail: seokmuiwang@yahoo.com. Sasheela A. Ponnampalavanar, Department of Medicine, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia, E-mail: sasheela@um.edu.my. Lucy Chai See Lum, Department of Pediatrics, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia, E-mail: lumcs@um.edu.my.

Reprint requests: Shamala Devi Sekaran, Department of Medical Microbiology, Faculty of Medicine, University of Malaya, Kuala Lumpur 50603, Malaysia, E-mail: shamalaya@yahoo.com.

Copyright:
© The American Society of Tropical Medicine and Hygiene 2012
Received:
30 Sep 2011
|
Accepted:
15 May 2012
|
Published Online:
07 Nov 2012
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
Online ISSN:
1476-1645
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