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-
1.
Stevenson LG, Drake SK, Murray PR, 2010. Rapid identification of bacteria in positive blood culture broths by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 48: 444– 447.
-
2.
Inglis TJ, Hodge M, Ketharanathan S, 2008. A hospital-wide study of the impact of introducing a personal data assistant-augmented blood culture round. J Med Microbiol 57: 43– 49.
-
3.
Hore C, 2001. Important unusual infections in Australia: a critical care perspective. Crit Care Resusc 3: 262– 272.
-
4.
Inglis TJ, Golledge CL, Clair A, Harvey J, 2001. Case report: recovery from persistent septicemic melioidosis. Am J Trop Med Hyg 65: 76– 82.
-
5.
Cheng AC, Currie BJ, 2005. Melioidosis: epidemiology, pathophysiology, and management. Clin Microbiol Rev 18: 383– 416.
-
6.
Inglis TJ, Chiang D, Lee GS, Chor-Kiang L, 1998. Potential misidentification of Burkholderia pseudomallei by API 20NE. Pathology 30: 62– 64.
-
7.
Inglis TJ, Merritt A, Chidlow A, Aravena-Roman M, Harnett G, 2005. Comparison of diagnostic laboratory methods for identification of Burkholderia pseudomallei. J Clin Microbiol 43: 2201– 2206.
-
8.
Inglis TJ, Aravena-Roman M, Ching S, Croft K, Wuthiekanun V, Mee BJ, 2003. Cellular fatty acid profile distinguishes Burkholderia pseudomallei from avirulent Burkholderia thailandensis. J Clin Microbiol 41: 4812– 4814.
-
9.
Inglis TJ, O'Reilly L, Merritt AJ, Levy A, Heath C, 2011. The aftermath of the Western Australian melioidosis outbreak. Am J Trop Med Hyg 84: 851– 857.
-
10.
Mukhopadhyay S, Thomason MK, Lentz S, Nolan N, Willner K, Gee J, Glass MB, Inglis TJ, Merritt A, Levy A, Sozhamannan S, Mateczun A, Read T, 2010. High-redundancy draft sequencing of 15 clinical and environmental Burkholderia strains. J Bacteriol 192: 6313– 6314.
-
11.
Al-Soud WA, Lantz P-G, Bäckman A, Olcén P, Rådström P, 1998. A sample preparation method which facilitates detection of bacteria in blood cultures by the polymerase chain reaction. J Microbiol Methods 32: 217– 224.
-
12.
Merritt A, Inglis TJ, Chidlow G, Harnett G, 2006. PCR-based identification of Burkholderia pseudomallei. Rev Inst Med Trop Sao Paulo 48: 239– 244.
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Use of Matrix-Assisted Laser Desorption/Ionization Time-of-Flight Mass Spectrometry Analysis for Rapid Confirmation of Burkholderia pseudomallei in Septicemic Melioidosis
Timothy J. J. Inglis
Timothy J. J. Inglis
School of Pathology and Laboratory Medicine, Faculty of Medicine, Dentistry, and Health Sciences, University of Western Australia, Nedlands 6009, Western Australia, Australia; Division of Microbiology and Infectious Diseases, PathWest Laboratory Medicine, Queen Elizabeth II Medical Centre, Nedlands 6009, Western Australia, Australia; Bruker Daltonics Division, Bruker Biosciences, Melbourne, Victoria, Australia
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Paul E. Healy
Paul E. Healy
School of Pathology and Laboratory Medicine, Faculty of Medicine, Dentistry, and Health Sciences, University of Western Australia, Nedlands 6009, Western Australia, Australia; Division of Microbiology and Infectious Diseases, PathWest Laboratory Medicine, Queen Elizabeth II Medical Centre, Nedlands 6009, Western Australia, Australia; Bruker Daltonics Division, Bruker Biosciences, Melbourne, Victoria, Australia
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Leith J. Fremlin
Leith J. Fremlin
School of Pathology and Laboratory Medicine, Faculty of Medicine, Dentistry, and Health Sciences, University of Western Australia, Nedlands 6009, Western Australia, Australia; Division of Microbiology and Infectious Diseases, PathWest Laboratory Medicine, Queen Elizabeth II Medical Centre, Nedlands 6009, Western Australia, Australia; Bruker Daltonics Division, Bruker Biosciences, Melbourne, Victoria, Australia
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Clayton L. Golledge
Clayton L. Golledge
School of Pathology and Laboratory Medicine, Faculty of Medicine, Dentistry, and Health Sciences, University of Western Australia, Nedlands 6009, Western Australia, Australia; Division of Microbiology and Infectious Diseases, PathWest Laboratory Medicine, Queen Elizabeth II Medical Centre, Nedlands 6009, Western Australia, Australia; Bruker Daltonics Division, Bruker Biosciences, Melbourne, Victoria, Australia
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Burkholderia pseudomallei was quickly identified from blood cultures collected from septicemic patients by matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis using an in-house reference library. This procedure reduced the time to definitive identification by more than 24 hours. This analysis is a useful addition to laboratory methods for early recognition of septicemic melioidosis in non-endemic settings.
Author Notes
Disclosure: Leith J. Fremlin is an employee of Bruker Biosciences, the manufacturers of the MALDI-TOF MS we used. His role in this work was to assist in the bioinformatic software analysis of MS results and reporting of that process as a scientist. This statement is made in the interest of full disclosure and not because the authors consider this to be a conflict of interest.
Authors' addresses: Timothy J. J. Inglis, School of Pathology and Laboratory Medicine, Faculty of Medicine, Dentistry, and Health Sciences, University of Western Australia, Nedlands 6009, Western Australia, Australia and Division of Microbiology and Infectious Diseases, PathWest Laboratory Medicine, Queen Elizabeth II Medical Centre, Nedlands 6009, Western Australia, Australia, E-mail: tim.inglis@health.wa.gov.au. Paul E. Healy and Clayton L. Golledge, Division of Microbiology and Infectious Diseases, PathWest Laboratory Medicine, Queen Elizabeth II Medical Centre, Nedlands 6009, Western Australia, Australia, E-mails: paul.healy@health.wa.gov.au and clay.gollege@health.wa.gov.au. Leith J. Fremlin, Bruker Daltonics Division, Bruker Biosciences, Melbourne, Victoria, Australia, E-mail: leith.fremlin@bruker-daltonics.com.au.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Stevenson LG, Drake SK, Murray PR, 2010. Rapid identification of bacteria in positive blood culture broths by matrix-assisted laser desorption ionization-time of flight mass spectrometry. J Clin Microbiol 48: 444– 447.
-
2.
Inglis TJ, Hodge M, Ketharanathan S, 2008. A hospital-wide study of the impact of introducing a personal data assistant-augmented blood culture round. J Med Microbiol 57: 43– 49.
-
3.
Hore C, 2001. Important unusual infections in Australia: a critical care perspective. Crit Care Resusc 3: 262– 272.
-
4.
Inglis TJ, Golledge CL, Clair A, Harvey J, 2001. Case report: recovery from persistent septicemic melioidosis. Am J Trop Med Hyg 65: 76– 82.
-
5.
Cheng AC, Currie BJ, 2005. Melioidosis: epidemiology, pathophysiology, and management. Clin Microbiol Rev 18: 383– 416.
-
6.
Inglis TJ, Chiang D, Lee GS, Chor-Kiang L, 1998. Potential misidentification of Burkholderia pseudomallei by API 20NE. Pathology 30: 62– 64.
-
7.
Inglis TJ, Merritt A, Chidlow A, Aravena-Roman M, Harnett G, 2005. Comparison of diagnostic laboratory methods for identification of Burkholderia pseudomallei. J Clin Microbiol 43: 2201– 2206.
-
8.
Inglis TJ, Aravena-Roman M, Ching S, Croft K, Wuthiekanun V, Mee BJ, 2003. Cellular fatty acid profile distinguishes Burkholderia pseudomallei from avirulent Burkholderia thailandensis. J Clin Microbiol 41: 4812– 4814.
-
9.
Inglis TJ, O'Reilly L, Merritt AJ, Levy A, Heath C, 2011. The aftermath of the Western Australian melioidosis outbreak. Am J Trop Med Hyg 84: 851– 857.
-
10.
Mukhopadhyay S, Thomason MK, Lentz S, Nolan N, Willner K, Gee J, Glass MB, Inglis TJ, Merritt A, Levy A, Sozhamannan S, Mateczun A, Read T, 2010. High-redundancy draft sequencing of 15 clinical and environmental Burkholderia strains. J Bacteriol 192: 6313– 6314.
-
11.
Al-Soud WA, Lantz P-G, Bäckman A, Olcén P, Rådström P, 1998. A sample preparation method which facilitates detection of bacteria in blood cultures by the polymerase chain reaction. J Microbiol Methods 32: 217– 224.
-
12.
Merritt A, Inglis TJ, Chidlow G, Harnett G, 2006. PCR-based identification of Burkholderia pseudomallei. Rev Inst Med Trop Sao Paulo 48: 239– 244.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 67 | 45 | 1 |
| Full Text Views | 290 | 7 | 0 |
| PDF Downloads | 44 | 3 | 0 |