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RefWorks
Reference Manager
BibTeX
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-
1
Adam RD, 1991. The biology of Giardia spp. Microbiol Rev 55 :706–732.
-
2
Ahmed MM, El-Hady M, Morsy TA, 1990. Parasitic infections and hemoglobin levels among children of different socioeconomic classes in Abha, Saudi-Arabia. J Egypt Soc Parasitol 20 :61–67.
-
3
Di Prisco MC, Jimenez JC, Rodriguez N, Costa V, Villamizar J, Silvera A, Carrillo M, Lira C, Zerpa E, Lopez Y, 2000. Clinical trial with secnidazole in a single dose in Venezuelan children infected by Giardia intestinalis. Invest Clin 41 :179–188.
-
4
Miller SA, Rosario CL, Rojas E, Scorza JV, 2003. Intestinal parasitic infection and associated symptoms in children attending day care centers in Trujillo, Venezuela. Trop Med Int Health 8 :342–347.
-
5
Heyworth MF, Vergara JA, 1994. Giardia muris trophozoite antigenic targets for mouse intestinal IgA antibody. J Infect Dis 69 :395–398.
-
6
Char S, Shetty N, Narasimha M, 1991. Serum antobidy response in children with Giardia lamblia and infection of an immunodominant 57-kilodalton antigen. Parasite Immunol 13 :329–337.
-
7
Char S, Cevallo AM, Yamson P, 1993. Impaired IgA response to Giardia heat-shock antigen in children with persistent diarrhoea and giardiasis. Gut 34 :38–40.
-
8
Cervetto JL, Ramonet M, Nahmod LH, 1987. Giardiasis. Functional, immunological and histological study of the small bowel. Therapeutic trial with a single dose of tinidazole. Arq Gastroenterol 24 :102–112.
-
9
Keister DB, 1983. Axenic culture of Giardia lamblia in TYI-S-33 medium supplemented with bile. Trans R Soc Trop Med Hyg 77 :487–488.
-
10
Laemmli UK, 1970. Cleavege of structural proteins during the assembly of the head of bactriophage T4. Nature 227 :680–685.
-
11
Towbin H, Staehlin T, Gordon J, 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76 :4350–4354.
-
12
Miotti PG, Gilman RH, Pickering LK, 1985. Prevalence of serum and milk antibodies to Giardia lamblia in different populations of lacting women. J Infect Dis 152 :1025–1031.
-
13
Rastegar-Lari A, Salek-Moghaddam A, 1996. Single-dose secnidazole versus 10-day metronidazole therapy of giardiasis in Iranian children. J Trop Pediatr 42 :184–185.
-
14
Cimerman BL, Camilo Coura C, Salle JM, Gurvitz R, Rocha RS, Bandeira S, Cimerman S, Katz N, 1997. Evaluation of secnidazole gel and tinidazole suspension in the treatment of giardiasis in children. Braz J Infect Dis 1 :241–247.
-
15
Escobedo AA, Canete R, Gonzalez ME, Pareja A, Cimerman S, Almirall P, 2003. A randomized trial comparing mebendazole and secnidazole for the treatment of giardiasis. Ann Trop Med Parasitol 97 :499–504.
-
16
Jimenez JC, Rodriguez N, Di Prisco MC, Lynch NR, Costa V, 1999. Haemoglobin concentrations and infection by Giardia intestinalis in children: effect of treatment with secnidazole. Ann Trop Med Parasitol 93 :823–827.
-
17
Granot E, Spira T, Fraser D, Deckelbaum RJ, 1998. Immunologic response to infection with Giardia lamblia in children: effect of different clinical settings. J Trop Pediatr 44 :241–246.
-
18
Hasan SMT, Maachee M, Córdova OM, Diaz de la Guardia R, Martins M, Osuna A, 2002. Human secretory immune response to fatty acid-binding protein fraction from Giardia lamblia. Infect Immun 70 :2226–2229.
-
19
Palm JE, Weiland ME, Griffiths WJ, Ljungstrom I, Svard SG, 2003. Identification of immunoreactive proteins during acute human giardiasis. J Infect Dis 187 :1849–1859.
-
20
Reiner DS, Gillin F, 1992. Human secretory and serum antibodies recognize environmentally induced antigens of Giardia lamblia. Infect Immun 60 :637–643.
-
21
Soliman MM, Ruhi Taghi-Kalani F, Ahmed A, Abou-Shady A, Salama A, El-Mageid A, Hondousa A, Hegazi MM, Belosevic M, 1998. Comparison of serum antibody responses to Giardia lamblia of symptomatic and asymptomatic patients. Am J Trop Med Hyg 58 :232–239.
-
22
Craig WR, Walker W, Alexander J, 2001. Sex-associated hormones and immunity to protozoan parasite. Clin Microbiol Rev 14 :476–488.
-
23
Bajeva UK, Warhust DC, 1983. Humoral response in giardiasis. J Commun Dis 15 :256.
-
24
Anam K, Afrin D, Banerjee N, Pramanik SK, Guha RP, Goswami SK, Saha N, Ali N, 1999. Differential decline in Leishmania membrane antigen-specific immunoglobulin G (IgG), IgM, IgE and IgG subclass antibodies in Indian kalaazar patients after chemotherapy. Infect Immun 67 :6663–6669.
-
25
Isaza DM, Restrepo M, Mosca W, 1997. Western blot analysis of Leishmania panamensis antigens in sera of patients with American cutaneous leishmaniasis. J Clin Microbiol 35 :3043–3047.
-
26
Kumar Pai PK, Tripathi K, Pandey HP, Sundar S, 2002. Western blot analysis of the humoral immune response to Leishmania donovani polypeptides in cases of human visceral leishmaniasis: its usefulness in prognosis. Clin Diag Lab Immunol 9 :1119–1123.
-
27
Mutapi FP, Ndhlovu D, Hagan P, 1998. Chemotherapy accelerates the development of acquired immune response to Schistosoma haematobium infection. J Infect Dis 178 :289–293.
-
28
Noya O, Losada S, Alarcón de Noya B, 1995. Effect of chemotherapy on immune response to egg antigens of Schistosoma mansoni in chronically infected children from areas of low transmission. Parasite Immunol 17 :111–117.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 193 | 160 | 5 |
| Full Text Views | 216 | 4 | 1 |
| PDF Downloads | 58 | 4 | 1 |
Antibody Response in Children Infected with Giardia intestinalis before and after Treatment with Secnidazole
Juan C. Jiménez
Juan C. Jiménez
Laboratory of Immunopathology, Institute of Biomedicine, Faculty of Medicine, University Central of Venezuela, Caracas, Venezuela; EA-3609 Parasitology-Mycology Service, Faculty of Medicine, Lille 2 University, Lille, France; Predictive Service Centre Européen de Recherches sur l’Eau, l’Environnement, l’Aliment et la Toxicologie, Institut Pasteur de Lille, Lille, France; Institute National de la Santé et de la Recherche Médicale Unite 547–Schistosomiasis, Malaria and Inflammation, Institut Pasteur de Lille, Université Lille 2, Lille, France
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Anthony Pinon
Anthony Pinon
Laboratory of Immunopathology, Institute of Biomedicine, Faculty of Medicine, University Central of Venezuela, Caracas, Venezuela; EA-3609 Parasitology-Mycology Service, Faculty of Medicine, Lille 2 University, Lille, France; Predictive Service Centre Européen de Recherches sur l’Eau, l’Environnement, l’Aliment et la Toxicologie, Institut Pasteur de Lille, Lille, France; Institute National de la Santé et de la Recherche Médicale Unite 547–Schistosomiasis, Malaria and Inflammation, Institut Pasteur de Lille, Université Lille 2, Lille, France
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Daniel Dive
Daniel Dive
Laboratory of Immunopathology, Institute of Biomedicine, Faculty of Medicine, University Central of Venezuela, Caracas, Venezuela; EA-3609 Parasitology-Mycology Service, Faculty of Medicine, Lille 2 University, Lille, France; Predictive Service Centre Européen de Recherches sur l’Eau, l’Environnement, l’Aliment et la Toxicologie, Institut Pasteur de Lille, Lille, France; Institute National de la Santé et de la Recherche Médicale Unite 547–Schistosomiasis, Malaria and Inflammation, Institut Pasteur de Lille, Université Lille 2, Lille, France
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Monique Capron
Monique Capron
Laboratory of Immunopathology, Institute of Biomedicine, Faculty of Medicine, University Central of Venezuela, Caracas, Venezuela; EA-3609 Parasitology-Mycology Service, Faculty of Medicine, Lille 2 University, Lille, France; Predictive Service Centre Européen de Recherches sur l’Eau, l’Environnement, l’Aliment et la Toxicologie, Institut Pasteur de Lille, Lille, France; Institute National de la Santé et de la Recherche Médicale Unite 547–Schistosomiasis, Malaria and Inflammation, Institut Pasteur de Lille, Université Lille 2, Lille, France
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Eduardo Dei-Cas
Eduardo Dei-Cas
Laboratory of Immunopathology, Institute of Biomedicine, Faculty of Medicine, University Central of Venezuela, Caracas, Venezuela; EA-3609 Parasitology-Mycology Service, Faculty of Medicine, Lille 2 University, Lille, France; Predictive Service Centre Européen de Recherches sur l’Eau, l’Environnement, l’Aliment et la Toxicologie, Institut Pasteur de Lille, Lille, France; Institute National de la Santé et de la Recherche Médicale Unite 547–Schistosomiasis, Malaria and Inflammation, Institut Pasteur de Lille, Université Lille 2, Lille, France
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Jacinto Convit
Jacinto Convit
Laboratory of Immunopathology, Institute of Biomedicine, Faculty of Medicine, University Central of Venezuela, Caracas, Venezuela; EA-3609 Parasitology-Mycology Service, Faculty of Medicine, Lille 2 University, Lille, France; Predictive Service Centre Européen de Recherches sur l’Eau, l’Environnement, l’Aliment et la Toxicologie, Institut Pasteur de Lille, Lille, France; Institute National de la Santé et de la Recherche Médicale Unite 547–Schistosomiasis, Malaria and Inflammation, Institut Pasteur de Lille, Université Lille 2, Lille, France
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We examined 364 school children for intestinal parasites in a sub-urban zone of Caracas, Venezuela. Giardia intestinalis was the most prevalent parasite in stool samples from 34 children. Levels of IgA and IgG antibodies to G. intestinalis were assessed by enzyme-linked immunosorbent assay and Western blot before and after treatment with secnidazole. All patients were cured with a reduction of IgA antibody levels in 26 of 34 children and a reduction in IgG-specific antibody levels in 18 of 34 children. Serum of infected patients reacted with proteins of 14 kD to 137 kD. Some patients did not show a change in IgA serum reactivity for parasite proteins by Western blot after treatment. Seventeen children showed reduction of the reactivity or disappearance of protein reactivity (mainly the 14-kD, 122-kD, and 137-kD proteins). Antibody response was not related to clinical status, but quantitative and qualitative serum antibody response against G. intestinalis infection could be used to assess levels of new protein markers that decrease or disappear with successful chemotherapy.
Author Notes
ProCite
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Reference Manager
BibTeX
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-
1
Adam RD, 1991. The biology of Giardia spp. Microbiol Rev 55 :706–732.
-
2
Ahmed MM, El-Hady M, Morsy TA, 1990. Parasitic infections and hemoglobin levels among children of different socioeconomic classes in Abha, Saudi-Arabia. J Egypt Soc Parasitol 20 :61–67.
-
3
Di Prisco MC, Jimenez JC, Rodriguez N, Costa V, Villamizar J, Silvera A, Carrillo M, Lira C, Zerpa E, Lopez Y, 2000. Clinical trial with secnidazole in a single dose in Venezuelan children infected by Giardia intestinalis. Invest Clin 41 :179–188.
-
4
Miller SA, Rosario CL, Rojas E, Scorza JV, 2003. Intestinal parasitic infection and associated symptoms in children attending day care centers in Trujillo, Venezuela. Trop Med Int Health 8 :342–347.
-
5
Heyworth MF, Vergara JA, 1994. Giardia muris trophozoite antigenic targets for mouse intestinal IgA antibody. J Infect Dis 69 :395–398.
-
6
Char S, Shetty N, Narasimha M, 1991. Serum antobidy response in children with Giardia lamblia and infection of an immunodominant 57-kilodalton antigen. Parasite Immunol 13 :329–337.
-
7
Char S, Cevallo AM, Yamson P, 1993. Impaired IgA response to Giardia heat-shock antigen in children with persistent diarrhoea and giardiasis. Gut 34 :38–40.
-
8
Cervetto JL, Ramonet M, Nahmod LH, 1987. Giardiasis. Functional, immunological and histological study of the small bowel. Therapeutic trial with a single dose of tinidazole. Arq Gastroenterol 24 :102–112.
-
9
Keister DB, 1983. Axenic culture of Giardia lamblia in TYI-S-33 medium supplemented with bile. Trans R Soc Trop Med Hyg 77 :487–488.
-
10
Laemmli UK, 1970. Cleavege of structural proteins during the assembly of the head of bactriophage T4. Nature 227 :680–685.
-
11
Towbin H, Staehlin T, Gordon J, 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76 :4350–4354.
-
12
Miotti PG, Gilman RH, Pickering LK, 1985. Prevalence of serum and milk antibodies to Giardia lamblia in different populations of lacting women. J Infect Dis 152 :1025–1031.
-
13
Rastegar-Lari A, Salek-Moghaddam A, 1996. Single-dose secnidazole versus 10-day metronidazole therapy of giardiasis in Iranian children. J Trop Pediatr 42 :184–185.
-
14
Cimerman BL, Camilo Coura C, Salle JM, Gurvitz R, Rocha RS, Bandeira S, Cimerman S, Katz N, 1997. Evaluation of secnidazole gel and tinidazole suspension in the treatment of giardiasis in children. Braz J Infect Dis 1 :241–247.
-
15
Escobedo AA, Canete R, Gonzalez ME, Pareja A, Cimerman S, Almirall P, 2003. A randomized trial comparing mebendazole and secnidazole for the treatment of giardiasis. Ann Trop Med Parasitol 97 :499–504.
-
16
Jimenez JC, Rodriguez N, Di Prisco MC, Lynch NR, Costa V, 1999. Haemoglobin concentrations and infection by Giardia intestinalis in children: effect of treatment with secnidazole. Ann Trop Med Parasitol 93 :823–827.
-
17
Granot E, Spira T, Fraser D, Deckelbaum RJ, 1998. Immunologic response to infection with Giardia lamblia in children: effect of different clinical settings. J Trop Pediatr 44 :241–246.
-
18
Hasan SMT, Maachee M, Córdova OM, Diaz de la Guardia R, Martins M, Osuna A, 2002. Human secretory immune response to fatty acid-binding protein fraction from Giardia lamblia. Infect Immun 70 :2226–2229.
-
19
Palm JE, Weiland ME, Griffiths WJ, Ljungstrom I, Svard SG, 2003. Identification of immunoreactive proteins during acute human giardiasis. J Infect Dis 187 :1849–1859.
-
20
Reiner DS, Gillin F, 1992. Human secretory and serum antibodies recognize environmentally induced antigens of Giardia lamblia. Infect Immun 60 :637–643.
-
21
Soliman MM, Ruhi Taghi-Kalani F, Ahmed A, Abou-Shady A, Salama A, El-Mageid A, Hondousa A, Hegazi MM, Belosevic M, 1998. Comparison of serum antibody responses to Giardia lamblia of symptomatic and asymptomatic patients. Am J Trop Med Hyg 58 :232–239.
-
22
Craig WR, Walker W, Alexander J, 2001. Sex-associated hormones and immunity to protozoan parasite. Clin Microbiol Rev 14 :476–488.
-
23
Bajeva UK, Warhust DC, 1983. Humoral response in giardiasis. J Commun Dis 15 :256.
-
24
Anam K, Afrin D, Banerjee N, Pramanik SK, Guha RP, Goswami SK, Saha N, Ali N, 1999. Differential decline in Leishmania membrane antigen-specific immunoglobulin G (IgG), IgM, IgE and IgG subclass antibodies in Indian kalaazar patients after chemotherapy. Infect Immun 67 :6663–6669.
-
25
Isaza DM, Restrepo M, Mosca W, 1997. Western blot analysis of Leishmania panamensis antigens in sera of patients with American cutaneous leishmaniasis. J Clin Microbiol 35 :3043–3047.
-
26
Kumar Pai PK, Tripathi K, Pandey HP, Sundar S, 2002. Western blot analysis of the humoral immune response to Leishmania donovani polypeptides in cases of human visceral leishmaniasis: its usefulness in prognosis. Clin Diag Lab Immunol 9 :1119–1123.
-
27
Mutapi FP, Ndhlovu D, Hagan P, 1998. Chemotherapy accelerates the development of acquired immune response to Schistosoma haematobium infection. J Infect Dis 178 :289–293.
-
28
Noya O, Losada S, Alarcón de Noya B, 1995. Effect of chemotherapy on immune response to egg antigens of Schistosoma mansoni in chronically infected children from areas of low transmission. Parasite Immunol 17 :111–117.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 193 | 160 | 5 |
| Full Text Views | 216 | 4 | 1 |
| PDF Downloads | 58 | 4 | 1 |