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-
1
Sotelo J, Guerrero V, Rubio F, 1985. Neurocysticercosis: a new classification based on active and inactive forms. A study of 753 cases. Arch Intern Med 145 :442–445.
-
2
Del Brutto OH, Wadia NH, Dumas M, Cruz M, Tsang VC, Schantz PM, 1996. Proposal of diagnostic criteria for human cysticercosis and neurocysticercosis. J Neurol Sci 142 :1–6.
-
3
White AC, 2000. Neurocysticercosis: updates on epidemiology, pathogenesis, diagnosis and management. Annu Rev Med 51 :187–206.
-
4
Tsang VCW, Brand JA, Boyer AE, 1989. An enzyme-linked immunoelectrotransfer blot assay and glycoprotein antigens for diagnosing human cysticercosis (Taenia solium). J Infect Dis 159 :50–59.
-
5
Wilson M, Bryan RT, Fried JA, Ware DA, Schantz PM, Pilcher JB, 1991. Clinical evaluation of the cysticercosis enzyme-linked immunoelectrotransfer blot in patients with neurocysticercosis. J Infect Dis 164 :1007–1009.
-
6
Chung J, Bahk Y, Huh S, Kang S, Kong Y, Cho S, 1999. A recombinant 10 kDa protein of Taenia solium metacestodes specific to active neurocysticercosis. J Infect Dis 180 :1307–1315.
-
7
Hubert K, Andriantsimahavandy A, Michault A, Forsch M, Muhlschlegel F, 1999. Serological diagnosis of human cysticercosis by use of recombinant antigens from Taenia solium cysticerci. Clin Diagn Lab Immunol 6 :479–482.
-
8
Sako Y, Nakao M, Ikejima T, Piao XZ, Nakaya K, Ito A, 2000. Molecular characterization and diagnostic value of Taenia solium low-molecular-weight antigen genes. J Clin Microbiol 38 :4439–4444.
-
9
Baily GG, Mason PR, Trijssenar FE, Lyons NF, 1988. Serological diagnosis of neurocysticercosis: evaluation of ELISA tests using cyst fluid and other components of Taenia solium cysticerci as antigens. Trans R Soc Trop Med Hyg 82 :295–299.
-
10
Estrada JJ, Estrada JA, Kuhn RE, 1989. Identification of Taenia solium antigens in cerebrospinal fluid and larval antigens from patients with neurocysticercosis. Am J Trop Med Hyg 41 :50–55.
-
11
Dekumyoy P, Anantaphruti MT, Nuamtanong S, Watthanakulpanich D, Waikagu J, Danis M, 2000. Neurocysticercosis: utilizing the cystic fluid antigen from Taenia solium metacestodes for diagnosis by IgG-ELISA. Southeast Asian J Trop Med Public Health 31 :21–25.
-
12
Bueno EC, Snege M, Vaz AJ, Leser PG, 2001. Serodiagnosis of human cysticercosis by using antigens from vesicular fluid of Taenia crassiceps cysticerci.Clin Diagn Lab Immunol 8 :1140–1144.
-
13
Ramos CM, Montoya RM, Padilla A, Govezensky T, Diaz ML, Sciutto E, Sotelo J, Larralde C, 1992. Immunodiagnosis of neurocysticercosis: disappointing performance of serology (enzyme-linked immunosorbent assay) in an unbiased sample of neurological patients. Arch Neurol 49 :633–636.
-
14
Restrepo BI, Obregón-Henao A, Mesa M, Gil DL, Ortiz BL, Mejía JS, Villota GE, Sanzón AF, Teale JM, 2000. Characterization of the carbohydrate components of Taenia solium metacestode glycoprotein antigens. Int J Parasitol 30 :689–696.
-
15
Greene R, Wilkins P, Tsang VW, 1999. Diagnostic glycoproteins of Taenia solium share homologous 14 and 18 kD subunits. Mol Biochem Parasitol 99 :257–261.
-
16
Obregón-Henao A, Gil DL, Gomez DI, Sanzon F, Teale JM, Restrepo BI, 2001. The role of N-linked carbohydrates in the antigenicity of Taenia solium metacestode glycoproteins of 12, 16 and 18 kD. Mol Biochem Parasitol 114 :209–215.
-
17
Bueno EC, Vaz AJ, Machado LD, Livramento JA, 2000. Neurocysticercosis: detection of IgG, IgA and IgE antibodies in cerebrospinal fluid, serum and saliva samples by ELISA with Taenia solium and Taenia crassiceps antigens. Arq Neuropsiquiatr 58 :18–24.
| Past two years | Past Year | Past 30 Days | |
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| Abstract Views | 318 | 293 | 28 |
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SIMILAR DIAGNOSTIC PERFORMANCE FOR NEUROCYSTICERCOSIS OF THREE GLYCOPROTEIN PREPARATIONS FROM TAENIA SOLIUM METACESTODES
GUIDO E. VILLOTA
GUIDO E. VILLOTA
Molecular Parasitology Group, Corporación para Investigaciones Biológicas, Medellín, Colombia; Facultad de Ciencias Pecuarias, Centro de Estudios en Salud, Universidad de Nariño, San Juan de Pasto, Colombia; Universidad de Antioquia and Hospital Universitario San Vicente de Paul, Medellín, Colombia; Neurology Service, Hospital Pablo Tobón Uribe, Medellín, Colombia; Department of Microbiology, University of Texas Health Science Center at San Antonio, San Antonio, Texas
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DIANA I. GOMEZ
DIANA I. GOMEZ
Molecular Parasitology Group, Corporación para Investigaciones Biológicas, Medellín, Colombia; Facultad de Ciencias Pecuarias, Centro de Estudios en Salud, Universidad de Nariño, San Juan de Pasto, Colombia; Universidad de Antioquia and Hospital Universitario San Vicente de Paul, Medellín, Colombia; Neurology Service, Hospital Pablo Tobón Uribe, Medellín, Colombia; Department of Microbiology, University of Texas Health Science Center at San Antonio, San Antonio, Texas
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MICHEL VOLCY
MICHEL VOLCY
Molecular Parasitology Group, Corporación para Investigaciones Biológicas, Medellín, Colombia; Facultad de Ciencias Pecuarias, Centro de Estudios en Salud, Universidad de Nariño, San Juan de Pasto, Colombia; Universidad de Antioquia and Hospital Universitario San Vicente de Paul, Medellín, Colombia; Neurology Service, Hospital Pablo Tobón Uribe, Medellín, Colombia; Department of Microbiology, University of Texas Health Science Center at San Antonio, San Antonio, Texas
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ANDRÉS F. FRANCO
ANDRÉS F. FRANCO
Molecular Parasitology Group, Corporación para Investigaciones Biológicas, Medellín, Colombia; Facultad de Ciencias Pecuarias, Centro de Estudios en Salud, Universidad de Nariño, San Juan de Pasto, Colombia; Universidad de Antioquia and Hospital Universitario San Vicente de Paul, Medellín, Colombia; Neurology Service, Hospital Pablo Tobón Uribe, Medellín, Colombia; Department of Microbiology, University of Texas Health Science Center at San Antonio, San Antonio, Texas
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EDGAR A. CARDONA
EDGAR A. CARDONA
Molecular Parasitology Group, Corporación para Investigaciones Biológicas, Medellín, Colombia; Facultad de Ciencias Pecuarias, Centro de Estudios en Salud, Universidad de Nariño, San Juan de Pasto, Colombia; Universidad de Antioquia and Hospital Universitario San Vicente de Paul, Medellín, Colombia; Neurology Service, Hospital Pablo Tobón Uribe, Medellín, Colombia; Department of Microbiology, University of Texas Health Science Center at San Antonio, San Antonio, Texas
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RODRIGO ISAZA
RODRIGO ISAZA
Molecular Parasitology Group, Corporación para Investigaciones Biológicas, Medellín, Colombia; Facultad de Ciencias Pecuarias, Centro de Estudios en Salud, Universidad de Nariño, San Juan de Pasto, Colombia; Universidad de Antioquia and Hospital Universitario San Vicente de Paul, Medellín, Colombia; Neurology Service, Hospital Pablo Tobón Uribe, Medellín, Colombia; Department of Microbiology, University of Texas Health Science Center at San Antonio, San Antonio, Texas
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FERNANDO SANZÓN
FERNANDO SANZÓN
Molecular Parasitology Group, Corporación para Investigaciones Biológicas, Medellín, Colombia; Facultad de Ciencias Pecuarias, Centro de Estudios en Salud, Universidad de Nariño, San Juan de Pasto, Colombia; Universidad de Antioquia and Hospital Universitario San Vicente de Paul, Medellín, Colombia; Neurology Service, Hospital Pablo Tobón Uribe, Medellín, Colombia; Department of Microbiology, University of Texas Health Science Center at San Antonio, San Antonio, Texas
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JUDY M. TEALE
JUDY M. TEALE
Molecular Parasitology Group, Corporación para Investigaciones Biológicas, Medellín, Colombia; Facultad de Ciencias Pecuarias, Centro de Estudios en Salud, Universidad de Nariño, San Juan de Pasto, Colombia; Universidad de Antioquia and Hospital Universitario San Vicente de Paul, Medellín, Colombia; Neurology Service, Hospital Pablo Tobón Uribe, Medellín, Colombia; Department of Microbiology, University of Texas Health Science Center at San Antonio, San Antonio, Texas
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BLANCA I. RESTREPO
BLANCA I. RESTREPO
Molecular Parasitology Group, Corporación para Investigaciones Biológicas, Medellín, Colombia; Facultad de Ciencias Pecuarias, Centro de Estudios en Salud, Universidad de Nariño, San Juan de Pasto, Colombia; Universidad de Antioquia and Hospital Universitario San Vicente de Paul, Medellín, Colombia; Neurology Service, Hospital Pablo Tobón Uribe, Medellín, Colombia; Department of Microbiology, University of Texas Health Science Center at San Antonio, San Antonio, Texas
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The detection of antibodies to Taenia solium metacestodes is very important in the differential diagnosis of neurocysticercosis (NCC). In this study, an electroimmunotransfer blot (EITB) assay that uses an elaborate protocol with metacestode glycoproteins as antigens was compared with two other Western blots that use glycoproteins obtained using simpler methods, including an eluate from a lectin column, or the vesicular fluid (VF) of the parasite. The concordance between the three assays was 91% in patients with active NCC and 100% in patients with suspected NCC and previous documentation of negative serology. The specificities for the Western blots and the EITB assay were 98% and 100%, respectively (98% concordance). These data suggest that the simplest of these immunoassays, the one that uses the VF of T. solium metacestodes in a Western blot format, can be reliably used for the serologic diagnosis of NCC in developing countries where access to the EITB assay is difficult.
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-
1
Sotelo J, Guerrero V, Rubio F, 1985. Neurocysticercosis: a new classification based on active and inactive forms. A study of 753 cases. Arch Intern Med 145 :442–445.
-
2
Del Brutto OH, Wadia NH, Dumas M, Cruz M, Tsang VC, Schantz PM, 1996. Proposal of diagnostic criteria for human cysticercosis and neurocysticercosis. J Neurol Sci 142 :1–6.
-
3
White AC, 2000. Neurocysticercosis: updates on epidemiology, pathogenesis, diagnosis and management. Annu Rev Med 51 :187–206.
-
4
Tsang VCW, Brand JA, Boyer AE, 1989. An enzyme-linked immunoelectrotransfer blot assay and glycoprotein antigens for diagnosing human cysticercosis (Taenia solium). J Infect Dis 159 :50–59.
-
5
Wilson M, Bryan RT, Fried JA, Ware DA, Schantz PM, Pilcher JB, 1991. Clinical evaluation of the cysticercosis enzyme-linked immunoelectrotransfer blot in patients with neurocysticercosis. J Infect Dis 164 :1007–1009.
-
6
Chung J, Bahk Y, Huh S, Kang S, Kong Y, Cho S, 1999. A recombinant 10 kDa protein of Taenia solium metacestodes specific to active neurocysticercosis. J Infect Dis 180 :1307–1315.
-
7
Hubert K, Andriantsimahavandy A, Michault A, Forsch M, Muhlschlegel F, 1999. Serological diagnosis of human cysticercosis by use of recombinant antigens from Taenia solium cysticerci. Clin Diagn Lab Immunol 6 :479–482.
-
8
Sako Y, Nakao M, Ikejima T, Piao XZ, Nakaya K, Ito A, 2000. Molecular characterization and diagnostic value of Taenia solium low-molecular-weight antigen genes. J Clin Microbiol 38 :4439–4444.
-
9
Baily GG, Mason PR, Trijssenar FE, Lyons NF, 1988. Serological diagnosis of neurocysticercosis: evaluation of ELISA tests using cyst fluid and other components of Taenia solium cysticerci as antigens. Trans R Soc Trop Med Hyg 82 :295–299.
-
10
Estrada JJ, Estrada JA, Kuhn RE, 1989. Identification of Taenia solium antigens in cerebrospinal fluid and larval antigens from patients with neurocysticercosis. Am J Trop Med Hyg 41 :50–55.
-
11
Dekumyoy P, Anantaphruti MT, Nuamtanong S, Watthanakulpanich D, Waikagu J, Danis M, 2000. Neurocysticercosis: utilizing the cystic fluid antigen from Taenia solium metacestodes for diagnosis by IgG-ELISA. Southeast Asian J Trop Med Public Health 31 :21–25.
-
12
Bueno EC, Snege M, Vaz AJ, Leser PG, 2001. Serodiagnosis of human cysticercosis by using antigens from vesicular fluid of Taenia crassiceps cysticerci.Clin Diagn Lab Immunol 8 :1140–1144.
-
13
Ramos CM, Montoya RM, Padilla A, Govezensky T, Diaz ML, Sciutto E, Sotelo J, Larralde C, 1992. Immunodiagnosis of neurocysticercosis: disappointing performance of serology (enzyme-linked immunosorbent assay) in an unbiased sample of neurological patients. Arch Neurol 49 :633–636.
-
14
Restrepo BI, Obregón-Henao A, Mesa M, Gil DL, Ortiz BL, Mejía JS, Villota GE, Sanzón AF, Teale JM, 2000. Characterization of the carbohydrate components of Taenia solium metacestode glycoprotein antigens. Int J Parasitol 30 :689–696.
-
15
Greene R, Wilkins P, Tsang VW, 1999. Diagnostic glycoproteins of Taenia solium share homologous 14 and 18 kD subunits. Mol Biochem Parasitol 99 :257–261.
-
16
Obregón-Henao A, Gil DL, Gomez DI, Sanzon F, Teale JM, Restrepo BI, 2001. The role of N-linked carbohydrates in the antigenicity of Taenia solium metacestode glycoproteins of 12, 16 and 18 kD. Mol Biochem Parasitol 114 :209–215.
-
17
Bueno EC, Vaz AJ, Machado LD, Livramento JA, 2000. Neurocysticercosis: detection of IgG, IgA and IgE antibodies in cerebrospinal fluid, serum and saliva samples by ELISA with Taenia solium and Taenia crassiceps antigens. Arq Neuropsiquiatr 58 :18–24.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 318 | 293 | 28 |
| Full Text Views | 230 | 2 | 0 |
| PDF Downloads | 39 | 2 | 0 |