ProCite
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Reference Manager
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-
1.
Moore DAJ, McCroddan J, Dekumyoy P, Chiodini PL, 2003. Gnathostomiasis: an emerging imported disease. Emerg Infect Dis 9: 647–650.
-
2.
Bravo F, Gontijo B, 2018. Gnathostomiasis: an emerging infectious disease relevant to all dermatologists. An Bras Dermatol 93: 172–180.
-
3.
Sanpool O, Intapan PM, Blair D, Nawa Y, Maleewong W, 2018. Gnathostoma. Liu D, ed. Handbook of Foodborne Diseases. Boca Raton, FL: CRC Press, Taylor & Francis Group, 859–869.
-
4.
Nomura Y, Nagakura K, Kagei N, Tsutsumi Y, Araki K, Sugawara M, 2000. Gnathostomiasis possibly caused by Gnathostoma malaysiae. Tokai J Exp Clin Med 25: 1–6.
-
5.
Maleewong W, Intapan PM, Khempila J, Wongwajana S, Wongkham C, Morakote N, 1995. Gnathostoma spinigerum: growth and development of third-stage larvae in vitro. J Parasitol 81: 800–803.
-
6.
Ando K, 2003. Gnathostoma japonicum. Kamegai N, Otsuru M, Hayashi S, eds. Progress in Medical Parasitology, Japan. Vol. 8. Tokyo, Japan: Meguro Parasite Museum, 507–518.
-
7.
Nawa Y, Intapan PM, Maleewong W, Camacho SP, 2015. Gnathostoma. Xiao L, Ryan U, Feng Y, eds. Biology of Foodborne Parasites. Boca Raton, FL: CRC Press, Taylor & Francis Group, 405–426.
-
8.
Phetsouvanh R, Habe S, Newton P, Vongsouvaht M, Horii Y, Doanh PN, Nawa Y, 2018. Spontaneous emergence of a Gnathostoma spinigerum adult worm from the abdominal skin: a Laotian woman: a case report. Southeast Asian J Trop Med Public Health 49: 1–5.
-
9.
Daengsvang S, 1980. Monograph on the genus Gnathostoma and gnathostomiasis in Thailand. Tokyo, Japan: SEAMIC Publication No. 21.
-
10.
Waikagul J, Diaz Chamacho SP, 2007. Gnathostomiasis. Murrell KD, Fried B, eds. Food-borne Parasitic Zoonoses. World Class Parasites, Vol. 11. New York, NY: Springer, 235–261.
-
11.
Seguchi K, Matsuno M, Kataoka H, Kobayashi T, Maruyama H, Itoh H, Koono M, Nawa Y, 1995. A case report of colonic ileus due to eosinophilic nodular lesions caused by Gnathostoma doloresi infection. Am J Trop Med Hyg 53: 263–266.
-
12.
Janwan P et al. 2016. Development and usefulness of an immunochromatographic device to detect antibodies for rapid diagnosis of human gnathostomiasis. Parasit Vectors 9: 14.
-
13.
Almeyda-Artigas RJ, Bargues MD, Mas-Coma S, 2000. ITS-2 rDNA sequencing of Gnathostoma species (Nematoda) and elucidation of the species causing human gnathostomiasis in the Americas. J Parasitol 86: 537–544.
-
14.
Boonroumkaew P et al. 2019. Molecular identification and genetic diversity of Gnathostoma spinigerum larvae in freshwater fishes in southern Lao PDR, Cambodia, and Myanmar. Parasitol Res 118: 1465–1472.
-
15.
Hall TA, 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symp Ser 41: 95–98.
-
16.
Kumar S, Stecher G, Li M, Knyaz C, Tamura K, 2018. MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molecular Mol Biol Evol 35: 1547–1549.
| Past two years | Past Year | Past 30 Days | |
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| Abstract Views | 2318 | 1323 | 87 |
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An Unusual Case of Gastric Gnathostomiasis Caused by Gnathostoma spinigerum Confirmed by Video Gastroscopy and Morphological and Molecular Identification
Kookwan Sawadpanich
Kookwan Sawadpanich
Gastroenterology and Hepatology Division, Department of Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;
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Nitiwat Chansuk
Nitiwat Chansuk
Internal Medicine Unit, Ratchaphruek Hospital, Khon Kaen, Thailand;
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Patcharaporn Boonroumkaew
Patcharaporn Boonroumkaew
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;
Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;
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Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;
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Lakkhana Sadaow
Lakkhana Sadaow
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;
Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;
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Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;
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Rutchanee Rodpai
Rutchanee Rodpai
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;
Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;
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Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;
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Oranuch Sanpool
Oranuch Sanpool
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;
Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;
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Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;
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Penchom Janwan
Penchom Janwan
Department of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat, Thailand
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Pewpan M. Intapan
Pewpan M. Intapan
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;
Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;
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Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;
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Wanchai Maleewong
Wanchai Maleewong
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;
Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;
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Abstract.
Human gnathostomiasis is a harmful foodborne parasitic infection caused by nematodes of the genus Gnathostoma. Here, we report an unusual case of gastric gnathostomiasis seen in a hospital in Thailand along with the clinical characteristics, treatment, and outcome. A 39-year-old man presented with complaints of epigastric pain, dizziness, and history of passing dark, tarry stools for 2 days. The patient had a history of consuming raw freshwater fish. Supplementary differential diagnosis was performed via rapid serological testing, and presence of the causative agent was confirmed based on video gastroscopy, morphology of the removed parasite, and molecular identification. After its surgical removal from the stomach, the parasite was morphologically identified as Gnathostoma species. Molecular identification was performed via DNA extraction from the recovered worm, and amplification and sequencing of the second internal transcribed spacer (ITS2) region and partial cytochrome c oxidase subunit I (cox1) gene. The ITS2 and cox1 sequences were consistent with those of Gnathostoma spinigerum. Clinicians in endemic areas should therefore be aware of the rare clinical manifestations and use of supplementary serological tests to facilitate early diagnosis and treatment of gastric gnathostomiasis.
- Supplemental Materials (MP4 1.68 MB)
Author Notes
Financial support: This study was supported by a Distinguished Research Professor Grant from the Thailand Research Fund [Grant no. DPG6280002], a scholarship under Industrial Post-doctorate Development for Agriculture, Food, Energy and Bio-materials for Future from Khon Kaen University, Thailand [PJ, grant number KKU-PMU-B 63-001], and grants from the Khon Kaen University Research and Graduate studies [RP64010] and Faculty of Medicine [WM and OS, grant numbers DR63101and RG63301]. The fund providers had no role in study design, data collection/interpretation, or the decision to submit the work for publication.
Authors’ addresses: Kookwan Sawadpanich, Gastroenterology and Hepatology Division, Department of Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand, E-mail: kookwans@yahoo.com. Nitiwat Chansuk, Internal Medicine Unit, Ratchaphruek Hospital, Khon Kaen, Thailand, E-mail: nitiwat_internist@hotmail.com. Patcharaporn Boonroumkaew, Lakkhana Sadaow, Rutchanee Rodapai, Oranuch Sanpool, Pewpan M. Intapan, and Wanchai Maleewong, Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand, E-mails: patcharaporn.bo@kkumail.com, sadaow1986@gmail.com, rutchanee5020@gmail.com, sanpoolor@yahoo.com, pewpan@kku.ac.th, and wanch_ma@kku.ac.th. Penchom Janwan, Department of Medical Technology, School of Allied Health Sciences, Walailak University, Nakhon Si Thammarat, Thailand, E-mail: pair.wu@gmail.com.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Moore DAJ, McCroddan J, Dekumyoy P, Chiodini PL, 2003. Gnathostomiasis: an emerging imported disease. Emerg Infect Dis 9: 647–650.
-
2.
Bravo F, Gontijo B, 2018. Gnathostomiasis: an emerging infectious disease relevant to all dermatologists. An Bras Dermatol 93: 172–180.
-
3.
Sanpool O, Intapan PM, Blair D, Nawa Y, Maleewong W, 2018. Gnathostoma. Liu D, ed. Handbook of Foodborne Diseases. Boca Raton, FL: CRC Press, Taylor & Francis Group, 859–869.
-
4.
Nomura Y, Nagakura K, Kagei N, Tsutsumi Y, Araki K, Sugawara M, 2000. Gnathostomiasis possibly caused by Gnathostoma malaysiae. Tokai J Exp Clin Med 25: 1–6.
-
5.
Maleewong W, Intapan PM, Khempila J, Wongwajana S, Wongkham C, Morakote N, 1995. Gnathostoma spinigerum: growth and development of third-stage larvae in vitro. J Parasitol 81: 800–803.
-
6.
Ando K, 2003. Gnathostoma japonicum. Kamegai N, Otsuru M, Hayashi S, eds. Progress in Medical Parasitology, Japan. Vol. 8. Tokyo, Japan: Meguro Parasite Museum, 507–518.
-
7.
Nawa Y, Intapan PM, Maleewong W, Camacho SP, 2015. Gnathostoma. Xiao L, Ryan U, Feng Y, eds. Biology of Foodborne Parasites. Boca Raton, FL: CRC Press, Taylor & Francis Group, 405–426.
-
8.
Phetsouvanh R, Habe S, Newton P, Vongsouvaht M, Horii Y, Doanh PN, Nawa Y, 2018. Spontaneous emergence of a Gnathostoma spinigerum adult worm from the abdominal skin: a Laotian woman: a case report. Southeast Asian J Trop Med Public Health 49: 1–5.
-
9.
Daengsvang S, 1980. Monograph on the genus Gnathostoma and gnathostomiasis in Thailand. Tokyo, Japan: SEAMIC Publication No. 21.
-
10.
Waikagul J, Diaz Chamacho SP, 2007. Gnathostomiasis. Murrell KD, Fried B, eds. Food-borne Parasitic Zoonoses. World Class Parasites, Vol. 11. New York, NY: Springer, 235–261.
-
11.
Seguchi K, Matsuno M, Kataoka H, Kobayashi T, Maruyama H, Itoh H, Koono M, Nawa Y, 1995. A case report of colonic ileus due to eosinophilic nodular lesions caused by Gnathostoma doloresi infection. Am J Trop Med Hyg 53: 263–266.
-
12.
Janwan P et al. 2016. Development and usefulness of an immunochromatographic device to detect antibodies for rapid diagnosis of human gnathostomiasis. Parasit Vectors 9: 14.
-
13.
Almeyda-Artigas RJ, Bargues MD, Mas-Coma S, 2000. ITS-2 rDNA sequencing of Gnathostoma species (Nematoda) and elucidation of the species causing human gnathostomiasis in the Americas. J Parasitol 86: 537–544.
-
14.
Boonroumkaew P et al. 2019. Molecular identification and genetic diversity of Gnathostoma spinigerum larvae in freshwater fishes in southern Lao PDR, Cambodia, and Myanmar. Parasitol Res 118: 1465–1472.
-
15.
Hall TA, 1999. BioEdit: a user-friendly biological sequence alignment editor and analysis program for windows 95/98/NT. Nucleic Acids Symp Ser 41: 95–98.
-
16.
Kumar S, Stecher G, Li M, Knyaz C, Tamura K, 2018. MEGA X: Molecular Evolutionary Genetics Analysis across computing platforms. Molecular Mol Biol Evol 35: 1547–1549.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 2318 | 1323 | 87 |
| Full Text Views | 886 | 77 | 3 |
| PDF Downloads | 596 | 21 | 3 |