Genchi C, Rinaldi L, Mortarino M, Genchi M, Cringoli G, 2009. Climate and Dirofilaria infection in Europe. Vet Parasitol 163: 286–292.
Simón F, Siles-Lucas M, Morchón R, Gonzalez-Miguel J, Mellado I, Carretón E, Montoya-Alonso JA, 2012. Human and animal dirofilariosis: the emergence of a zoonotic mosaic. Clin Microbiol Rev 25: 507–544.
Lechner AM, Gastager H, Kern JM, Wagner B, Tappe D, 2020. Case report: successful treatment of a patient with microfilaremic dirofilariasis using doxycycline. Am J Trop Med Hyg 102: 844–846.
Kini RG, Leena JB, Shetty P, Lyngdoh RH, Sumanth D, Lovely G, 2015. Human dirofilariosis: an emerging zoonosis in India. J Parasit Dis 39: 349–354.
Sabu L, Devada K, Subramanian H, 2005. Dirofilariosis in dogs and humans in Kerala. Indian J Med Res 121: 691–693.
To KK et al. 2012. A novel Dirofilaria species causing human and canine infections in Hong Kong. J Clin Microbiol 50: 3534–3541.
Pradeep RK et al. 2019. Whether Dirofilaria repens parasites from South India belong to zoonotic Candidatus Dirofilaria hongkongensis (Dirofilaria sp. hongkongensis)? Infect Genet Evol 67: 121–125.
Winkler S, Pollreisz A, Georgopoulos M, Bagò-Horvath Z, Auer H, To KK, Krücken J, Poppert S, Walochnik J, 2017. Candidatus Dirofilaria hongkongensis as causative agent of human ocular filariosis after travel to India. Emerg Infect Dis 23: 1428–1431.
Gioia G, Lecová L, Genchi M, Ferri E, Genchi C, Mortarino M, 2010. Highly sensitive multiplex PCR for simultaneous detection and discrimination of Dirofilariai mmitis and Dirofilaria repens in canine peripheral blood. Vet Parasitol 172: 160–163.
Tamura K, Nei M, 1993. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 10: 512–526.
Nazar N, Lakshmanan B, Jayavardhanan KK, 2017. Molecular characterization of human Dirofilaria isolates from Kerala. Indian J Med Res 146: 528–533.
Sadarama PV, Chirayath D, Pillai UN, Unny NM, Lakshmanan B, Sunanda C, 2019. Canine Brugia malayi microfilarial excretory/secretory protein-based antibody assay for the diagnosis of Brugian filariasis in dogs. J Parasit Dis 43: 549–553.
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We tried to determine the epidemiology and species of human dirofilariasis observed at two tertiary care hospitals in Kerala. We searched the hospital database to identify cases of dirofilariosis from January 2005 to March 2020. Along with human isolates, one dog Dirofilaria isolate was also subjected to PCR and sequencing of pan filarial primers cytochrome oxidase subunits 1 and 12S rDNA. We documented 78 cases of human dirofilariosis. The orbit, eyelid, and conjunctiva were the most commonly affected sites. Molecular characterization identified one dog and five human isolates as Candidatus Dirofilaria Hongkongensis. A rare case of subconjunctival infestation by B. malayi was also documented. Human dirofilariosis is a public health problem in the state of Kerala in India, and it is mostly caused by Candidatus Dirofilaria Hongkongensis. We propose that all diroifilaria isolates are subjected to sequencing for identification.
Financial support: This research received no specific grant from any funding source.
Disclosure: No conflict of interest declared.
Authors’ addresses: Anil Kumar, Lalitha Biswas, Sarga Prabhat, Parasmal Suresh, Anisha Asokan, Vivek Vinod, Ajit Nambiar, and Raja Biswas, Amrita Institute of Medical Sciences and Research Centre, Molecular Biology, Cochin, Kerala, India, E-mails: vanilkumar@aims.amrita.edu, biswaslalitha@gmail.com, microbiology@aims.amrita.edu, parasmals@aims.amrita.edu, anishaka@gmail.com, vivekvinod@aims.amrita.edu, ajitn@aims.amrita.edu, and rajabiswas@aims.amrita.edu. Ani Sreedhar and Rita Mary Tomy, Little Flower Hospital and Research Centre, Ophthalmology, Angamaly, Kerala, India, E-mails: anisreedhar2002@yahoo.com and ritamarytomy@gmail.com. Bindu Lakshmanan, College of Veterinary and Animal Sciences Mannuthy, Veterinary Parasitology, Mannuthy, Kerala, India, E-mail: bindul@kvasu.ac.in.
Genchi C, Rinaldi L, Mortarino M, Genchi M, Cringoli G, 2009. Climate and Dirofilaria infection in Europe. Vet Parasitol 163: 286–292.
Simón F, Siles-Lucas M, Morchón R, Gonzalez-Miguel J, Mellado I, Carretón E, Montoya-Alonso JA, 2012. Human and animal dirofilariosis: the emergence of a zoonotic mosaic. Clin Microbiol Rev 25: 507–544.
Lechner AM, Gastager H, Kern JM, Wagner B, Tappe D, 2020. Case report: successful treatment of a patient with microfilaremic dirofilariasis using doxycycline. Am J Trop Med Hyg 102: 844–846.
Kini RG, Leena JB, Shetty P, Lyngdoh RH, Sumanth D, Lovely G, 2015. Human dirofilariosis: an emerging zoonosis in India. J Parasit Dis 39: 349–354.
Sabu L, Devada K, Subramanian H, 2005. Dirofilariosis in dogs and humans in Kerala. Indian J Med Res 121: 691–693.
To KK et al. 2012. A novel Dirofilaria species causing human and canine infections in Hong Kong. J Clin Microbiol 50: 3534–3541.
Pradeep RK et al. 2019. Whether Dirofilaria repens parasites from South India belong to zoonotic Candidatus Dirofilaria hongkongensis (Dirofilaria sp. hongkongensis)? Infect Genet Evol 67: 121–125.
Winkler S, Pollreisz A, Georgopoulos M, Bagò-Horvath Z, Auer H, To KK, Krücken J, Poppert S, Walochnik J, 2017. Candidatus Dirofilaria hongkongensis as causative agent of human ocular filariosis after travel to India. Emerg Infect Dis 23: 1428–1431.
Gioia G, Lecová L, Genchi M, Ferri E, Genchi C, Mortarino M, 2010. Highly sensitive multiplex PCR for simultaneous detection and discrimination of Dirofilariai mmitis and Dirofilaria repens in canine peripheral blood. Vet Parasitol 172: 160–163.
Tamura K, Nei M, 1993. Estimation of the number of nucleotide substitutions in the control region of mitochondrial DNA in humans and chimpanzees. Mol Biol Evol 10: 512–526.
Nazar N, Lakshmanan B, Jayavardhanan KK, 2017. Molecular characterization of human Dirofilaria isolates from Kerala. Indian J Med Res 146: 528–533.
Sadarama PV, Chirayath D, Pillai UN, Unny NM, Lakshmanan B, Sunanda C, 2019. Canine Brugia malayi microfilarial excretory/secretory protein-based antibody assay for the diagnosis of Brugian filariasis in dogs. J Parasit Dis 43: 549–553.
Past two years | Past Year | Past 30 Days | |
---|---|---|---|
Abstract Views | 2151 | 1450 | 78 |
Full Text Views | 431 | 43 | 1 |
PDF Downloads | 194 | 15 | 1 |