ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Cover TL, Blaser MJ, 2009. Helicobacter pylori in health and disease. Gastroenterology 136: 1863–1873.
-
2.
Pei Z, Yang L, Peek RM, Jr Levine SM, Pride DT, Blaser MJ, 2005. Bacterial biota in reflux esophagitis and Barrett’s esophagus. World J Gastroenterol 11: 7277–7283.
-
3.
Cellini L, Grande R, Artese L, Marzio L, 2010. Detection of Helicobacter pylori in saliva and esophagus. New Microbiol 33: 351–357.
-
4.
Contreras M, Salazar V, García-Amado M, Reyes N, Aparcero M, Silva O, Castro D, Romero R, Gueneau P, Michelangeli F, 2012. High frequency of Helicobacter pylori in the esophageal mucosa of dyspeptic patients and its possible association with histopathological alterations. Int J Infect Dis 16: e364–e370.
-
5.
Contreras M, Abrante L, Salazar V, Reyes N, García-Amado M, Fernández-Delgado M, Romero R, Rojas H, Michelangeli F, 2014. Heterogeneity of cag genotypes of Helicobacter pylori in the esophageal mucosa of dyspeptic patients and its relation to histopathological outcomes. Int J Infect Dis 26: 91–95.
-
6.
Peña J, Rojas H, Reyes N, Fernández-Delgado M, García-Amado M-A, Michelangeli F, Contreras M, 2017. Multiple cag genotypes of Helicobacter pylori isolates colonize the oesophagus in individual hosts in a Venezuelan population. J Med Microbiol 66: 226–235.
-
7.
Blaser MJ, 2012. Heterogeneity of Helicobacter pylori. Eur J Gastroenterol Hepatol 9 (Suppl 1): S3–S6; discussion S6–S7.
-
8.
Gerrits MM, van Vliet AHM, Kuipers EJ, Kusters JG, 2006. Helicobacter pylori and antimicrobial resistance: molecular mechanisms and clinical implications. Lancet Infect Dis 6: 699–709.
-
9.
Chu YT, Wang YH, Wu JJ, Lei HY, 2010. Invasion and multiplication of Helicobacter pylori in gastric epithelial cells and implications for antibiotic resistance. Infect Immun 78: 4157–4165.
-
10.
Dib J, Alvarez B, Mendez L, Cruz M, 2013. Efficacy of PPI, levofloxacin and amoxicillin in the eradication of Helicobacter pylori compared to conventional triple therapy at a Venezuelan hospital. Arab J Gastroenterol 14: 123–125.
-
11.
Wang LH, Cheng H, Hu FL, Li J, 2010. Distribution of gyrA mutations in fluoroquinolone resistant Helicobacter pylori strains. World J Gastroenterol 16: 2272–2277.
-
12.
Chung JW, Lee GH, Jeong JY, Lee SM, Jung JH, Choi KD, Song HJ, Jung HY, Kim JH, 2012. Resistance of Helicobacter pylori strains to antibiotics in Korea with a focus on fluoroquinolone resistance. J Gastroenterol Hepatol 27: 493–497.
-
13.
Domínguez-Bello MG et al. 1997. Modification of Christensen urease test as an inexpensive tool for detection of Helicobacter pylori. Diagn Microbiol Infect Dis 28: 149–152.
-
14.
Peek RM Jr, Miller GG, Tham KT, Pérez-Pérez GI, Cover TL, Atherton JC, Dunn GD, Blaser MJ, 1995. Detection of Helicobacter pylori gene expression in human gastric mucosa. J Clin Microbiol 33: 28–32.
-
15.
Kansau I, Raymond J, Bingen E, Courcoux P, Kalach N, Bergeret M, Braimi N, Dupont C, Labigne A, 1996. Genotyping of Helicobacter pylori isolates by sequencing of PCR products and comparison with the RAPD technique. Res Microbiol 147: 661–669.
-
16.
Germani Y, Dauga C, Duval P, Huerre M, Levy M, Pialoux G, Sansonetti P, Grimont PA, 1997. Strategy for the detection of Helicobacter species by amplification of 16S rRNA genes and identification of H. felis in human gastric biopsy. Res Microbiol 148: 315–326.
-
17.
Miyachi H et al. 2006. Primary levofloxacin resistance and gyrA/B mutations among Helicobacter pylori in Japan. Helicobacter 11: 243–249.
-
18.
Nishizawa T, Suzuki H, Umezawa A, Muraoka H, Iwasaki E, Masaoka T, Kobayashi I, Hibi T, 2007. Rapid detection of point mutations conferring resistance to fluoroquinolone in gyrA of Helicobacter pylori by allele-specific PCR. J Clin Microbiol 45: 303–305.
-
19.
Cuadrado-Lavin A et al. 2012. Antimicrobial susceptibility of Helicobacter pylori to six antibiotics currently used in Spain. J Antimicrob Chemother 67: 170–173.
-
20.
Tay CY, Windsor HM, Thirriot F, Lu W, Conway C, Perkins TT, Marshall BJ, 2012. Helicobacter pylori eradication in Western Australia using novel quadruple therapy combinations. Aliment Pharmacol Ther 36: 1076–1083.
-
21.
Fathi MS, EL-Folly RF, Hassan RA, El-Arab ME, 2013. Genotypic and phenotypic patterns of antimicrobial susceptibility of Helicobacter pylori strains among Egyptian patients. Egypt J Med Hum Genet 14: 235–246.
-
22.
Almeida N, Romãozinho JM, Donato MM, Luxo C, Cardoso O, Cipriano MA, Marinho C, Fernandes A, Calhau C, Sofia C, 2014. Helicobacter pylori antimicrobial resistance rates in the central region of Portugal. Clin Microbiol Infect 20: 1127–1133.
-
23.
Boltin D, Ben-Zvi H, Perets TT, Kamenetsky Z, Samra Z, Dickman R, Niv Y, 2015. Trends in secondary antibiotic resistance of Helicobacter pylori from 2007 to 2014: has the tide turned? J Clin Microbiol 53: 522–527.
-
24.
Caliskan R et al. 2015. Antimicrobial resistance of Helicobacter pylori strains to five antibiotics, including levofloxacin, in northwestern Turkey. Rev Soc Bras Med Trop 48: 278–284.
-
25.
Zaki M, Othman W, Ali MA, Shehta A, 2016. Fluoroquinolone-resistant Helicobacter pylori strains isolated from one Egyptian University Hospital: molecular aspects. J Microbiol Antimicrobial Agents 2: 26–31.
-
26.
Eisig JN, Silva FM, Barbuti RC, Navarro-Rodriguez T, Morales-Filho JP, Pedrazzoli P, 2011. Helicobacter pylori antibiotic resistance in Brazil: clarithromycin is still a good option. Arq Gastroenterol 48: 261–264.
-
27.
Mochizuki-Tamayo H, Noriega-Aldave AP, 2011. Antimicrobial susceptibility of Helicobacter pylori to levofloxacin determined in a miniwell format and disk diffusion tests using egg yolk agar. Rev Gastroenterol Peru 31: 224–229.
-
28.
Zerbetto De Palma G, Mendiondo N, Wonaga A, Viola L, Ibarra D, Campitelli E, Salim N, Corti R, Goldman C, Catalano M, 2016. Occurrence of mutations in the antimicrobial target genes related to levofloxacin, clarithromycin, and amoxicillin resistance in Helicobacter pylori isolates from Buenos Aires city. Microb Drug Resist 23: 351–358.
-
29.
Garcia M, Raymond J, Garnier M, Cremniter J, Burucoa C, 2012. Distribution of spontaneous gyrA mutations in 97 fluoroquinolone-resistant Helicobacter pylori isolates collected in France. Antimicrob Agents Chemother 56: 550–551.
-
30.
Seck A, Burucoa C, Dia D, Mbengue M, Onambele M, Raymond J, Breurec S, 2013. Primary antibiotic resistance and associated mechanisms in Helicobacter pylori isolates from Senegalese patients. Ann Clin Microbiol Antimicrob 12: 3.
-
31.
Tanih NF, Ndip RN, 2013. Molecular detection of antibiotic resistance in South African isolates of Helicobacter pylori. Gastroenterol Res Pract 2013: 259457.
-
32.
Trespalacios AA, Rimbara E, Otero W, Reddy R, Graham DY, 2015. Improved allele-specific PCR assays for detection of clarithromycin and fluoroquinolone resistant of Helicobacter pylori in gastric biopsies: identification of N87I mutation in gyrA. Diagn Microbiol Infect Dis 81: 251–255.
-
33.
Trespalacios-Rangél AA, Otero W, Arévalo-Galvis A, Poutou-Piñales RA, Rimbara E, Graham D, 2016. Surveillance of levofloxacin resistance in Helicobacter pylori isolates in Bogotá-Colombia (2009–2014). PLoS One 11: e0160007.
-
34.
Tankovic J, Lascols C, Sculo Q, Petit JC, Soussy JJ, 2003. Single and double mutations in gyrA but not in gyrB are associated with low- and high-level fluoroquinolone resistance in Helicobacter pylori. Antimicrob Agents Chemother 47: 3942–3944.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 494 | 398 | 28 |
| Full Text Views | 790 | 20 | 1 |
| PDF Downloads | 248 | 15 | 1 |
Point Mutations at gyrA and gyrB Genes of Levofloxacin-Resistant Helicobacter pylori Isolates in the Esophageal Mucosa from a Venezuelan Population
Mariela López-Gasca
Mariela López-Gasca
Laboratorio de Fisiología Gastrointestinal, Centro de Biofísica y Bioquímica, Instituto Venezolano de Investigaciones Científicas (IVIC), Miranda, Venezuela
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Jessy Peña
Jessy Peña
Laboratorio de Fisiología Gastrointestinal, Centro de Biofísica y Bioquímica, Instituto Venezolano de Investigaciones Científicas (IVIC), Miranda, Venezuela
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María-Alexandra García-Amado
María-Alexandra García-Amado
Laboratorio de Fisiología Gastrointestinal, Centro de Biofísica y Bioquímica, Instituto Venezolano de Investigaciones Científicas (IVIC), Miranda, Venezuela
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Fabián Michelangeli
Fabián Michelangeli
Laboratorio de Fisiología Gastrointestinal, Centro de Biofísica y Bioquímica, Instituto Venezolano de Investigaciones Científicas (IVIC), Miranda, Venezuela
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Monica Contreras
Monica Contreras
Laboratorio de Fisiología Gastrointestinal, Centro de Biofísica y Bioquímica, Instituto Venezolano de Investigaciones Científicas (IVIC), Miranda, Venezuela
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The treatment of Helicobacter pylori infection is complicated by antibiotic resistance. A high levofloxacin (LVX) resistance rate was previously demonstrated in H. pylori isolates from gastric mucosa (40%) and esophagus (19%) in individual hosts of a Venezuelan population. We aimed to assess the molecular mechanisms of LVX resistance and susceptibility in isolates from the gastroesophageal mucosa, by studying point mutations in the quinolone resistance–determining region of gyrA and gyrB genes. Sequencing of gyrA and gyrB genes (N = 120) helped to identify point mutations in 60 isolates (30 from antrum and 30 from esophagus) of five dyspeptic patients. Double (Asn87Thr and Asp91Asn) and single (Asn87Ile or Asn87Thr) mutations in the gyrA gene were identified in the esophageal mucosa. These mutations have been commonly found in the stomach. Occurrence of a single (Asn87Ile) mutation was associated with high resistance (minimum inhibitory concentration ≥ 32 μg/mL) to LVX. Only a single (Ser479Gly) mutation was found in the gyrB gene in both mucosae. One patient presented isolates with no mutations in the two genes studied. Isolates with the same mutation pattern in individual hosts revealed identical genetic profiles for these genes, confirming that isolates identified in the esophageal mucosa come from isolates colonizing the stomach. Helicobacter pylori resistance to LVX in the esophagus is related to double- and single-point mutations in gyrA and gyrB genes, such as those found in the stomach. Levofloxacin should be applied with caution, because its antibiotic effect on H. pylori is decreasing in Latin America, perhaps owing to high prescription rates.
Author Notes
Financial support: This work was supported by Instituto Venezolano de Investigaciones Científicas (IVIC).
Authors’ addresses: Mariela López-Gasca, Jessy Peña, María-Alexandra García-Amado, Fabián Michelangeli, and Monica Contreras, Laboratorio de Fisiología Gastrointestinal, Centro de Biofísica y Bioquímica, Instituto Venezolano de Investigaciones Científicas (IVIC), Miranda, Venezuela, E-mails: mvlopez@ivic.gob.ve, jessykarina.pena@gmail.com, magarcia@ivic.gob.ve, fmichelangeli@gmail.com, and mocontre@ivic.gob.ve.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Cover TL, Blaser MJ, 2009. Helicobacter pylori in health and disease. Gastroenterology 136: 1863–1873.
-
2.
Pei Z, Yang L, Peek RM, Jr Levine SM, Pride DT, Blaser MJ, 2005. Bacterial biota in reflux esophagitis and Barrett’s esophagus. World J Gastroenterol 11: 7277–7283.
-
3.
Cellini L, Grande R, Artese L, Marzio L, 2010. Detection of Helicobacter pylori in saliva and esophagus. New Microbiol 33: 351–357.
-
4.
Contreras M, Salazar V, García-Amado M, Reyes N, Aparcero M, Silva O, Castro D, Romero R, Gueneau P, Michelangeli F, 2012. High frequency of Helicobacter pylori in the esophageal mucosa of dyspeptic patients and its possible association with histopathological alterations. Int J Infect Dis 16: e364–e370.
-
5.
Contreras M, Abrante L, Salazar V, Reyes N, García-Amado M, Fernández-Delgado M, Romero R, Rojas H, Michelangeli F, 2014. Heterogeneity of cag genotypes of Helicobacter pylori in the esophageal mucosa of dyspeptic patients and its relation to histopathological outcomes. Int J Infect Dis 26: 91–95.
-
6.
Peña J, Rojas H, Reyes N, Fernández-Delgado M, García-Amado M-A, Michelangeli F, Contreras M, 2017. Multiple cag genotypes of Helicobacter pylori isolates colonize the oesophagus in individual hosts in a Venezuelan population. J Med Microbiol 66: 226–235.
-
7.
Blaser MJ, 2012. Heterogeneity of Helicobacter pylori. Eur J Gastroenterol Hepatol 9 (Suppl 1): S3–S6; discussion S6–S7.
-
8.
Gerrits MM, van Vliet AHM, Kuipers EJ, Kusters JG, 2006. Helicobacter pylori and antimicrobial resistance: molecular mechanisms and clinical implications. Lancet Infect Dis 6: 699–709.
-
9.
Chu YT, Wang YH, Wu JJ, Lei HY, 2010. Invasion and multiplication of Helicobacter pylori in gastric epithelial cells and implications for antibiotic resistance. Infect Immun 78: 4157–4165.
-
10.
Dib J, Alvarez B, Mendez L, Cruz M, 2013. Efficacy of PPI, levofloxacin and amoxicillin in the eradication of Helicobacter pylori compared to conventional triple therapy at a Venezuelan hospital. Arab J Gastroenterol 14: 123–125.
-
11.
Wang LH, Cheng H, Hu FL, Li J, 2010. Distribution of gyrA mutations in fluoroquinolone resistant Helicobacter pylori strains. World J Gastroenterol 16: 2272–2277.
-
12.
Chung JW, Lee GH, Jeong JY, Lee SM, Jung JH, Choi KD, Song HJ, Jung HY, Kim JH, 2012. Resistance of Helicobacter pylori strains to antibiotics in Korea with a focus on fluoroquinolone resistance. J Gastroenterol Hepatol 27: 493–497.
-
13.
Domínguez-Bello MG et al. 1997. Modification of Christensen urease test as an inexpensive tool for detection of Helicobacter pylori. Diagn Microbiol Infect Dis 28: 149–152.
-
14.
Peek RM Jr, Miller GG, Tham KT, Pérez-Pérez GI, Cover TL, Atherton JC, Dunn GD, Blaser MJ, 1995. Detection of Helicobacter pylori gene expression in human gastric mucosa. J Clin Microbiol 33: 28–32.
-
15.
Kansau I, Raymond J, Bingen E, Courcoux P, Kalach N, Bergeret M, Braimi N, Dupont C, Labigne A, 1996. Genotyping of Helicobacter pylori isolates by sequencing of PCR products and comparison with the RAPD technique. Res Microbiol 147: 661–669.
-
16.
Germani Y, Dauga C, Duval P, Huerre M, Levy M, Pialoux G, Sansonetti P, Grimont PA, 1997. Strategy for the detection of Helicobacter species by amplification of 16S rRNA genes and identification of H. felis in human gastric biopsy. Res Microbiol 148: 315–326.
-
17.
Miyachi H et al. 2006. Primary levofloxacin resistance and gyrA/B mutations among Helicobacter pylori in Japan. Helicobacter 11: 243–249.
-
18.
Nishizawa T, Suzuki H, Umezawa A, Muraoka H, Iwasaki E, Masaoka T, Kobayashi I, Hibi T, 2007. Rapid detection of point mutations conferring resistance to fluoroquinolone in gyrA of Helicobacter pylori by allele-specific PCR. J Clin Microbiol 45: 303–305.
-
19.
Cuadrado-Lavin A et al. 2012. Antimicrobial susceptibility of Helicobacter pylori to six antibiotics currently used in Spain. J Antimicrob Chemother 67: 170–173.
-
20.
Tay CY, Windsor HM, Thirriot F, Lu W, Conway C, Perkins TT, Marshall BJ, 2012. Helicobacter pylori eradication in Western Australia using novel quadruple therapy combinations. Aliment Pharmacol Ther 36: 1076–1083.
-
21.
Fathi MS, EL-Folly RF, Hassan RA, El-Arab ME, 2013. Genotypic and phenotypic patterns of antimicrobial susceptibility of Helicobacter pylori strains among Egyptian patients. Egypt J Med Hum Genet 14: 235–246.
-
22.
Almeida N, Romãozinho JM, Donato MM, Luxo C, Cardoso O, Cipriano MA, Marinho C, Fernandes A, Calhau C, Sofia C, 2014. Helicobacter pylori antimicrobial resistance rates in the central region of Portugal. Clin Microbiol Infect 20: 1127–1133.
-
23.
Boltin D, Ben-Zvi H, Perets TT, Kamenetsky Z, Samra Z, Dickman R, Niv Y, 2015. Trends in secondary antibiotic resistance of Helicobacter pylori from 2007 to 2014: has the tide turned? J Clin Microbiol 53: 522–527.
-
24.
Caliskan R et al. 2015. Antimicrobial resistance of Helicobacter pylori strains to five antibiotics, including levofloxacin, in northwestern Turkey. Rev Soc Bras Med Trop 48: 278–284.
-
25.
Zaki M, Othman W, Ali MA, Shehta A, 2016. Fluoroquinolone-resistant Helicobacter pylori strains isolated from one Egyptian University Hospital: molecular aspects. J Microbiol Antimicrobial Agents 2: 26–31.
-
26.
Eisig JN, Silva FM, Barbuti RC, Navarro-Rodriguez T, Morales-Filho JP, Pedrazzoli P, 2011. Helicobacter pylori antibiotic resistance in Brazil: clarithromycin is still a good option. Arq Gastroenterol 48: 261–264.
-
27.
Mochizuki-Tamayo H, Noriega-Aldave AP, 2011. Antimicrobial susceptibility of Helicobacter pylori to levofloxacin determined in a miniwell format and disk diffusion tests using egg yolk agar. Rev Gastroenterol Peru 31: 224–229.
-
28.
Zerbetto De Palma G, Mendiondo N, Wonaga A, Viola L, Ibarra D, Campitelli E, Salim N, Corti R, Goldman C, Catalano M, 2016. Occurrence of mutations in the antimicrobial target genes related to levofloxacin, clarithromycin, and amoxicillin resistance in Helicobacter pylori isolates from Buenos Aires city. Microb Drug Resist 23: 351–358.
-
29.
Garcia M, Raymond J, Garnier M, Cremniter J, Burucoa C, 2012. Distribution of spontaneous gyrA mutations in 97 fluoroquinolone-resistant Helicobacter pylori isolates collected in France. Antimicrob Agents Chemother 56: 550–551.
-
30.
Seck A, Burucoa C, Dia D, Mbengue M, Onambele M, Raymond J, Breurec S, 2013. Primary antibiotic resistance and associated mechanisms in Helicobacter pylori isolates from Senegalese patients. Ann Clin Microbiol Antimicrob 12: 3.
-
31.
Tanih NF, Ndip RN, 2013. Molecular detection of antibiotic resistance in South African isolates of Helicobacter pylori. Gastroenterol Res Pract 2013: 259457.
-
32.
Trespalacios AA, Rimbara E, Otero W, Reddy R, Graham DY, 2015. Improved allele-specific PCR assays for detection of clarithromycin and fluoroquinolone resistant of Helicobacter pylori in gastric biopsies: identification of N87I mutation in gyrA. Diagn Microbiol Infect Dis 81: 251–255.
-
33.
Trespalacios-Rangél AA, Otero W, Arévalo-Galvis A, Poutou-Piñales RA, Rimbara E, Graham D, 2016. Surveillance of levofloxacin resistance in Helicobacter pylori isolates in Bogotá-Colombia (2009–2014). PLoS One 11: e0160007.
-
34.
Tankovic J, Lascols C, Sculo Q, Petit JC, Soussy JJ, 2003. Single and double mutations in gyrA but not in gyrB are associated with low- and high-level fluoroquinolone resistance in Helicobacter pylori. Antimicrob Agents Chemother 47: 3942–3944.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 494 | 398 | 28 |
| Full Text Views | 790 | 20 | 1 |
| PDF Downloads | 248 | 15 | 1 |