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RefWorks
Reference Manager
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-
1.
Jacobson RL, 2003. Leishmania tropica (Kinetoplastida: Trypanosomatidae)—a perplexing parasite. Folia Parasitol (Praha) 50: 241–250.
-
2.
Handler MZ, Patel PA, Kapila R, Al-Qubati Y, Schwartz RA, 2015. Cutaneous and mucocutaneous leishmaniasis clinical perspectives. J Am Acad Dermatol 73: 897–910.
-
3.
Gandacu D, Glazer Y, Anis E, Karakis I, Warshavsky B, Slater P, Grotto I, 2014. Resurgence of cutaneous leishmaniasis in Israel, 2001–2012. Emerg Infect Dis 20: 1605–1611.
-
4.
Vinitsky O, Ore L, Habiballa H, Dar MC, 2010. Geographic and epidemiologic analysis of the cutaneous leishmaniasis outbreak in northern Israel, 2000–2003. Isr Med Assoc J 12: 652–656.
-
5.
Singer SR, Abramson N, Shoob H, Zaken O, Zentner G, Stein-Zamir C, 2008. Ecoepidemiology of cutaneous leishmaniasis outbreak, Israel. Emerg Infect Dis 14: 1424–1426.
-
6.
Talmi-Frank D, Jaffe CL, Nasereddin A, Warburg A, King R, Svobodova M, Peleg O, Baneth G, 2010. Leishmania tropica in rock hyraxes (Procavia capensis) in a focus of human cutaneous leishmaniasis. Am J Trop Med Hyg 82: 814–818.
-
7.
Svobodova M, Volf P, Votypka J, 2006. Experimental transmission of Leishmania tropica to hyraxes (Procavia capensis) by the bite of Phlebotomus arabicus. Microbes Infect 8: 1691–1694.
-
8.
Jacobson RL et al. 2003. Outbreak of cutaneous leishmaniasis in northern Israel. J Infect Dis 188: 1065–1073.
-
9.
Svobodova M et al. 2006. Distinct transmission cycles of Leishmania tropica in 2 adjacent foci, northern Israel. Emerg Infect Dis 12: 1860–1868.
-
10.
Savioli L, Velayudhan R, 2014. Small bite, big threat: World Health Day 2014. East Mediterr Health J 20: 217–218.
-
11.
Lestinova T, Rohousova I, Sima M, de Oliveira CI, Volf P, 2017. Insights into the sand fly saliva: blood-feeding and immune interactions between sand flies, hosts, and Leishmania. PLoS Negl Trop Dis 11: e0005600.
-
12.
de Vries HJC, Reedijk SH, Schallig H, 2015. Cutaneous leishmaniasis: recent developments in diagnosis and management. Am J Clin Dermatol 16: 99–109.
-
13.
Elsafi SH, Evans DA, 1989. A comparison of the direct agglutination test and enzyme-linked immunosorbent assay in the serodiagnosis of leishmaniasis in the Sudan. Trans R Soc Trop Med Hyg 83: 334–337.
-
14.
Al-Salem WS et al. 2014. Detection of high levels of anti-alpha-galactosyl antibodies in sera of patients with old world cutaneous leishmaniasis: a possible tool for diagnosis and biomarker for cure in an elimination setting. Parasitology 141: 1898–1903.
-
15.
Costa LE et al. 2016. New serological tools for improved diagnosis of human tegumentary leishmaniasis. J Immunol Methods 434: 39–45.
-
16.
Celeste BJ, Sanchez MCA, Ramos-Sanchez EM, Castro LGM, Costa FAL, Goto H, 2014. Recombinant Leishmania infantum heat shock protein 83 for the serodiagnosis of cutaneous, mucosal, and visceral leishmaniases. Am J Trop Med Hyg 90: 860–865.
-
17.
Rohousova I, Ozensoy S, Ozbel Y, Volf P, 2005. Detection of species-specific antibody response of humans and mice bitten by sand flies. Parasitology 130: 493–499.
-
18.
Kravchenko V, Wasserberg G, Warburg A, 2004. Bionomics of phlebotomine sandflies in the Galilee focus of cutaneous leishmaniasis in northern Israel. Med Vet Entomol 18: 418–428.
-
19.
Volf P, Rohousová I, 2001. Species-specific antigens in salivary glands of phlebotomine sandflies. Parasitology 122: 37–41.
-
20.
Drahota J, Lipoldova M, Volf P, Rohousova I, 2009. Specificity of anti-saliva immune response in mice repeatedly bitten by Phlebotomus sergenti. Parasite Immunol 31: 766–770.
| Past two years | Past Year | Past 30 Days | |
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| Abstract Views | 195 | 170 | 17 |
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Serological Evaluation of Cutaneous Leishmania tropica Infection in Northern Israel
Iva Rohoušová
Iva Rohoušová
Department of Parasitology, Charles University, Faculty of Science, Prague, Czech Republic;
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Dalit Talmi-Frank
Dalit Talmi-Frank
School of Veterinary Medicine, Hebrew University, Rehovot, Israel;
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Michaela Vlková
Michaela Vlková
Department of Parasitology, Charles University, Faculty of Science, Prague, Czech Republic;
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Tatiana Spitzová
Tatiana Spitzová
Department of Parasitology, Charles University, Faculty of Science, Prague, Czech Republic;
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Koranit Rishpon
Koranit Rishpon
Department of Pediatrics, Carmel Medical Center, Haifa, Israel;
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Charles L. Jaffe
Charles L. Jaffe
Department of Microbiology and Molecular Genetics, The Institute for Medical Research Israel-Canada, The Kuvin Centre for the Study of Infectious and Tropical Diseases, The Hebrew University - Hadassah Medical School, The Hebrew University of Jerusalem, Jerusalem, Israel
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Petr Volf
Petr Volf
Department of Parasitology, Charles University, Faculty of Science, Prague, Czech Republic;
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Gad Baneth
Gad Baneth
School of Veterinary Medicine, Hebrew University, Rehovot, Israel;
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Moshe Ephros
Moshe Ephros
Department of Pediatrics, Carmel Medical Center, Haifa, Israel;
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Leishmania spp. are medically important unicellular parasites transmitted by phlebotomine sand flies. The World Health Organization recently highlighted the importance of reliable diagnostic tools for leishmaniasis. Our study of human infection was conducted in two endemic foci of Leishmania tropica in the Galilee region, northern Israel. Elevated anti-Leishmania antibodies were present in the majority (78.6%) of L. tropica-PCR positive individuals. Moreover, the enzyme-linked immunosorbent assay showed high sensitivity, specificity, and negative and positive predictive values (ranging between 73% and 79%), thus fulfilling the basic requirement for future development of a serodiagnostic and screening tool. The anti-sand fly saliva antibodies used as biomarkers of exposure reflected the composition of the local sand fly fauna as well as the abundance of individual species. High levels of antibodies against vector salivary proteins may further indicate frequent exposure to sand flies and consequently a higher probability of Leishmania transmission.
- Supplemental Materials (PDF 455 KB)
Author Notes
Financial support: This work was partially supported by Charles University, the Czech Republic (UNCE 204013, http://www.cuni.cz/UKEN-1.html). The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Authors’ addresses: Iva Rohoušová, Tatiana Spitzová, and Petr Volf, Department of Parasitology, Charles University, Faculty of Science, Prague, Czech Republic, E-mails: kolarova2011@gmail.com, tatiana.spitzova@gmail.com, and volf@cesnet.cz. Dalit Talmi-Frank and Gad Baneth, Koret School of Veterinary Medicine, The Hebrew University, Rehovot, Israel, E-mails: dalitvet@gmail.com and gad.baneth@mail.huji.ac.il. Michaela Vlková, Genome Plasticity and Disease Group, Mater Medical Research Institute, Translation Research Institute Level 4, Woolloongabba, Queensland, Australia, E-mail: michaelakindlova84@gmail.com. Koranit Rishpon, Psychiatric Department, Rambam Medical Center, Haifa, Israel, E-mail: k_rishpon@rambam.health.gov.il. Charles L. Jaffe, Department of Microbiology and Molecular Genetics, The Institute for Medical Research Israel-Canada, The Kuvin Centre for the Study of Infectious and Tropical Diseases, The Hebrew University - Hadassah Medical School, The Hebrew University of Jerusalem, Jerusalem, Israel, E-mail: cjaffe@cc.huji.ac.il. Moshe Ephros, Department of Pediatrics, Carmel Medical Center, Haifa, Israel, E-mail: mefrat@technion.ac.il.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Jacobson RL, 2003. Leishmania tropica (Kinetoplastida: Trypanosomatidae)—a perplexing parasite. Folia Parasitol (Praha) 50: 241–250.
-
2.
Handler MZ, Patel PA, Kapila R, Al-Qubati Y, Schwartz RA, 2015. Cutaneous and mucocutaneous leishmaniasis clinical perspectives. J Am Acad Dermatol 73: 897–910.
-
3.
Gandacu D, Glazer Y, Anis E, Karakis I, Warshavsky B, Slater P, Grotto I, 2014. Resurgence of cutaneous leishmaniasis in Israel, 2001–2012. Emerg Infect Dis 20: 1605–1611.
-
4.
Vinitsky O, Ore L, Habiballa H, Dar MC, 2010. Geographic and epidemiologic analysis of the cutaneous leishmaniasis outbreak in northern Israel, 2000–2003. Isr Med Assoc J 12: 652–656.
-
5.
Singer SR, Abramson N, Shoob H, Zaken O, Zentner G, Stein-Zamir C, 2008. Ecoepidemiology of cutaneous leishmaniasis outbreak, Israel. Emerg Infect Dis 14: 1424–1426.
-
6.
Talmi-Frank D, Jaffe CL, Nasereddin A, Warburg A, King R, Svobodova M, Peleg O, Baneth G, 2010. Leishmania tropica in rock hyraxes (Procavia capensis) in a focus of human cutaneous leishmaniasis. Am J Trop Med Hyg 82: 814–818.
-
7.
Svobodova M, Volf P, Votypka J, 2006. Experimental transmission of Leishmania tropica to hyraxes (Procavia capensis) by the bite of Phlebotomus arabicus. Microbes Infect 8: 1691–1694.
-
8.
Jacobson RL et al. 2003. Outbreak of cutaneous leishmaniasis in northern Israel. J Infect Dis 188: 1065–1073.
-
9.
Svobodova M et al. 2006. Distinct transmission cycles of Leishmania tropica in 2 adjacent foci, northern Israel. Emerg Infect Dis 12: 1860–1868.
-
10.
Savioli L, Velayudhan R, 2014. Small bite, big threat: World Health Day 2014. East Mediterr Health J 20: 217–218.
-
11.
Lestinova T, Rohousova I, Sima M, de Oliveira CI, Volf P, 2017. Insights into the sand fly saliva: blood-feeding and immune interactions between sand flies, hosts, and Leishmania. PLoS Negl Trop Dis 11: e0005600.
-
12.
de Vries HJC, Reedijk SH, Schallig H, 2015. Cutaneous leishmaniasis: recent developments in diagnosis and management. Am J Clin Dermatol 16: 99–109.
-
13.
Elsafi SH, Evans DA, 1989. A comparison of the direct agglutination test and enzyme-linked immunosorbent assay in the serodiagnosis of leishmaniasis in the Sudan. Trans R Soc Trop Med Hyg 83: 334–337.
-
14.
Al-Salem WS et al. 2014. Detection of high levels of anti-alpha-galactosyl antibodies in sera of patients with old world cutaneous leishmaniasis: a possible tool for diagnosis and biomarker for cure in an elimination setting. Parasitology 141: 1898–1903.
-
15.
Costa LE et al. 2016. New serological tools for improved diagnosis of human tegumentary leishmaniasis. J Immunol Methods 434: 39–45.
-
16.
Celeste BJ, Sanchez MCA, Ramos-Sanchez EM, Castro LGM, Costa FAL, Goto H, 2014. Recombinant Leishmania infantum heat shock protein 83 for the serodiagnosis of cutaneous, mucosal, and visceral leishmaniases. Am J Trop Med Hyg 90: 860–865.
-
17.
Rohousova I, Ozensoy S, Ozbel Y, Volf P, 2005. Detection of species-specific antibody response of humans and mice bitten by sand flies. Parasitology 130: 493–499.
-
18.
Kravchenko V, Wasserberg G, Warburg A, 2004. Bionomics of phlebotomine sandflies in the Galilee focus of cutaneous leishmaniasis in northern Israel. Med Vet Entomol 18: 418–428.
-
19.
Volf P, Rohousová I, 2001. Species-specific antigens in salivary glands of phlebotomine sandflies. Parasitology 122: 37–41.
-
20.
Drahota J, Lipoldova M, Volf P, Rohousova I, 2009. Specificity of anti-saliva immune response in mice repeatedly bitten by Phlebotomus sergenti. Parasite Immunol 31: 766–770.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 195 | 170 | 17 |
| Full Text Views | 369 | 11 | 1 |
| PDF Downloads | 103 | 13 | 1 |