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-
1.
Amornchai P, Chierakul W, Wuthiekanun V, Mahakhunkijcharoen Y, Phetsouvanh R, Currie BJ, Newton PN, van Vinh Chau N, Wongratanacheewin S, Day NP, Peacock SJ, 2007. Accuracy of Burkholderia pseudomallei identification using the API 20NE system and a latex agglutination test. J Clin Microbiol 45: 3774–3776.
-
2.
Anuntagool N, Naigowit P, Petkanchanapong V, Aramsri P, Panichakul T, Sirisinha S, 2000. Monoclonal antibody-based rapid identification of Burkholderia pseudomallei in blood culture fluid from patients with community-acquired septicaemia. J Med Microbiol 49: 1075–1078.
-
3.
Samosornsuk N, Lulitanond A, Saenla N, Anuntagool N, Wongratanacheewin S, Sirisinha S, 1999. Short report: evaluation of a monoclonal antibody-based latex agglutination test for rapid diagnosis of septicemic melioidosis. Am J Trop Med Hyg 61: 735–737.
-
4.
Wuthiekanun V, Anuntagool N, White NJ, Sirisinha S, 2002. Short report: a rapid method for the differentiation of Burkholderia pseudomallei and Burkholderia thailandensis. Am J Trop Med Hyg 66: 759–761.
-
5.
McCormick JB, Weaver RE, Hayes PS, Boyce JM, Feldman RA, 1977. Wound infection by an indigenous Pseudomonas pseudomallei-like organism isolated from the soil: case report and epidemiologic study. J Infect Dis 135: 103–107.
-
6.
Nussbaum JJ, Hull DS, Carter MJ, 1980. Pseudomonas pseudomallei in an anopthalmic orbit. Arch Ophthalmol 98: 1224–1225.
-
7.
Meng Z, Li Z, Jin J, Zhang Y, Liu X, Yiang X, Ren H, 1988. Studies on fermented corn flour poisoning in rural areas of China. I. Epidemiology, clinical manifestations, and pathology. Biomed Environ Sci 1: 101–104.
-
8.
Dursun A, Zenciroglu A, Karagol BS, Hakan N, Okumus N, Gol N, Tanir G, 2012. Burkholderia gladioli sepsis in newborns. Eur J Pediatr 171: 1503–1509.
-
9.
Ross JP, Holland SM, Gill VJ, DeCarlo ES, Gallin JI, 1995. Severe Burkholderia (Pseudomonas) gladioli infection in chronic granulomatous disease: report of two successfully treated cases. Clin Infect Dis 21: 1291–1293.
-
10.
Price EP, Sarovich DS, Mayo M, Tuanyok A, Drees KP, Kaestli M, Beckstrom-Sternberg SM, Babic-Sternberg JS, Kidd TJ, Bell SC, Keim P, Pearson T, Currie BJ, 2013. Within-host evolution of Burkholderia pseudomallei over a twelve-year chronic carriage infection. MBio 4: e00388.
-
11.
Cuccui J, Milne TS, Harmer N, George AJ, Harding SV, Dean RE, Scott AE, Sarkar-Tyson M, Wren BW, Titball RW, Prior JL, 2012. Characterization of the Burkholderia pseudomallei K96243 capsular polysaccharide I coding region. Infect Immun 80: 1209–1221.
-
12.
Sim BM, Chantratita N, Ooi WF, Nandi T, Tewhey R, Wuthiekanun V, Thaipadungpanit J, Tumapa S, Ariyaratne P, Sung WK, Sem XH, Chua HH, Ramnarayanan K, Lin CH, Liu Y, Feil EJ, Glass MB, Tan G, Peacock SJ, Tan P, 2010. Genomic acquisition of a capsular polysaccharide virulence cluster by non-pathogenic Burkholderia isolates. Genome Biol 11: R89.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 616 | 497 | 51 |
| Full Text Views | 756 | 22 | 1 |
| PDF Downloads | 329 | 18 | 1 |
Evaluation of a Latex Agglutination Assay for the Identification of Burkholderia pseudomallei and Burkholderia mallei
Brea D. Duval
Brea D. Duval
Bacterial Special Pathogens Branch, Centers for Disease Control and Prevention, Atlanta, Georgia; Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
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Mindy G. Elrod
Mindy G. Elrod
Bacterial Special Pathogens Branch, Centers for Disease Control and Prevention, Atlanta, Georgia; Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
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Jay E. Gee
Jay E. Gee
Bacterial Special Pathogens Branch, Centers for Disease Control and Prevention, Atlanta, Georgia; Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
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Narisara Chantratita
Narisara Chantratita
Bacterial Special Pathogens Branch, Centers for Disease Control and Prevention, Atlanta, Georgia; Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
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Sarunporn Tandhavanant
Sarunporn Tandhavanant
Bacterial Special Pathogens Branch, Centers for Disease Control and Prevention, Atlanta, Georgia; Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
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Direk Limmathurotsakul
Direk Limmathurotsakul
Bacterial Special Pathogens Branch, Centers for Disease Control and Prevention, Atlanta, Georgia; Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
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Alex R. Hoffmaster
Alex R. Hoffmaster
Bacterial Special Pathogens Branch, Centers for Disease Control and Prevention, Atlanta, Georgia; Department of Microbiology and Immunology, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Mahidol-Oxford Tropical Medicine Research Unit, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand; Department of Tropical Hygiene, Faculty of Tropical Medicine, Mahidol University, Bangkok, Thailand
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Cases of melioidosis and glanders are rare in the United States, but the etiologic agents of each disease (Burkholderia pseudomallei and Burkholderia mallei, respectively) are classified as Tier 1 select agents because of concerns about their potential use as bioterrorism agents. A rapid, highly sensitive, and portable assay for clinical laboratories and field use is required. Our laboratory has further evaluated a latex agglutination assay for its ability to identify B. pseudomallei and B. mallei isolates. This assay uses a monoclonal antibody that specifically recognizes the capsular polysaccharide produced by B. pseudomallei and B. mallei, but is absent in closely related Burkholderia species. A total of 110 B. pseudomallei and B. mallei were tested, and 36 closely related Burkholderia species. The latex agglutination assay was positive for 109 of 110 (99.1% sensitivity) B. pseudomallei and B. mallei isolates tested.
Author Notes
Authors' addresses: Brea D. Duval, Mindy G. Elrod, Jay E. Gee, and Alex R. Hoffmaster, Centers for Disease Control and Prevention, Atlanta, GA, E-mails: BDuval@cdc.gov, MGElrod@cdc.gov, JGee1@cdc.gov, and AHoffmaster@cdc.gov. Narisara Chantratita, Sarunporn Tandhavanant, and Direk Limmathurotsakul, Mahidol University, Bangkok, Thailand, E-mails: Narisara@tropmedres.ac, Sarunporn@tropmedres.ac, and Direk@tropmedres.ac.
ProCite
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Reference Manager
BibTeX
Zotero
EndNote
-
1.
Amornchai P, Chierakul W, Wuthiekanun V, Mahakhunkijcharoen Y, Phetsouvanh R, Currie BJ, Newton PN, van Vinh Chau N, Wongratanacheewin S, Day NP, Peacock SJ, 2007. Accuracy of Burkholderia pseudomallei identification using the API 20NE system and a latex agglutination test. J Clin Microbiol 45: 3774–3776.
-
2.
Anuntagool N, Naigowit P, Petkanchanapong V, Aramsri P, Panichakul T, Sirisinha S, 2000. Monoclonal antibody-based rapid identification of Burkholderia pseudomallei in blood culture fluid from patients with community-acquired septicaemia. J Med Microbiol 49: 1075–1078.
-
3.
Samosornsuk N, Lulitanond A, Saenla N, Anuntagool N, Wongratanacheewin S, Sirisinha S, 1999. Short report: evaluation of a monoclonal antibody-based latex agglutination test for rapid diagnosis of septicemic melioidosis. Am J Trop Med Hyg 61: 735–737.
-
4.
Wuthiekanun V, Anuntagool N, White NJ, Sirisinha S, 2002. Short report: a rapid method for the differentiation of Burkholderia pseudomallei and Burkholderia thailandensis. Am J Trop Med Hyg 66: 759–761.
-
5.
McCormick JB, Weaver RE, Hayes PS, Boyce JM, Feldman RA, 1977. Wound infection by an indigenous Pseudomonas pseudomallei-like organism isolated from the soil: case report and epidemiologic study. J Infect Dis 135: 103–107.
-
6.
Nussbaum JJ, Hull DS, Carter MJ, 1980. Pseudomonas pseudomallei in an anopthalmic orbit. Arch Ophthalmol 98: 1224–1225.
-
7.
Meng Z, Li Z, Jin J, Zhang Y, Liu X, Yiang X, Ren H, 1988. Studies on fermented corn flour poisoning in rural areas of China. I. Epidemiology, clinical manifestations, and pathology. Biomed Environ Sci 1: 101–104.
-
8.
Dursun A, Zenciroglu A, Karagol BS, Hakan N, Okumus N, Gol N, Tanir G, 2012. Burkholderia gladioli sepsis in newborns. Eur J Pediatr 171: 1503–1509.
-
9.
Ross JP, Holland SM, Gill VJ, DeCarlo ES, Gallin JI, 1995. Severe Burkholderia (Pseudomonas) gladioli infection in chronic granulomatous disease: report of two successfully treated cases. Clin Infect Dis 21: 1291–1293.
-
10.
Price EP, Sarovich DS, Mayo M, Tuanyok A, Drees KP, Kaestli M, Beckstrom-Sternberg SM, Babic-Sternberg JS, Kidd TJ, Bell SC, Keim P, Pearson T, Currie BJ, 2013. Within-host evolution of Burkholderia pseudomallei over a twelve-year chronic carriage infection. MBio 4: e00388.
-
11.
Cuccui J, Milne TS, Harmer N, George AJ, Harding SV, Dean RE, Scott AE, Sarkar-Tyson M, Wren BW, Titball RW, Prior JL, 2012. Characterization of the Burkholderia pseudomallei K96243 capsular polysaccharide I coding region. Infect Immun 80: 1209–1221.
-
12.
Sim BM, Chantratita N, Ooi WF, Nandi T, Tewhey R, Wuthiekanun V, Thaipadungpanit J, Tumapa S, Ariyaratne P, Sung WK, Sem XH, Chua HH, Ramnarayanan K, Lin CH, Liu Y, Feil EJ, Glass MB, Tan G, Peacock SJ, Tan P, 2010. Genomic acquisition of a capsular polysaccharide virulence cluster by non-pathogenic Burkholderia isolates. Genome Biol 11: R89.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 616 | 497 | 51 |
| Full Text Views | 756 | 22 | 1 |
| PDF Downloads | 329 | 18 | 1 |