Molecular Evidence of Trichostrongylus colubriformis and Trichostrongylus axei Infections in Humans from Thailand and Lao PDR

Issarapong Phosuk Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Faculty of Medicine, Mahasarakham University, Mahasarakham, Thailand; Department of Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand

Search for other papers by Issarapong Phosuk in
Current site
Google Scholar
PubMed Close
,
Pewpan M. Intapan Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Faculty of Medicine, Mahasarakham University, Mahasarakham, Thailand; Department of Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand

Search for other papers by Pewpan M. Intapan in
Current site
Google Scholar
PubMed Close
,
Oranuch Sanpool Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Faculty of Medicine, Mahasarakham University, Mahasarakham, Thailand; Department of Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand

Search for other papers by Oranuch Sanpool in
Current site
Google Scholar
PubMed Close
,
Penchom Janwan Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Faculty of Medicine, Mahasarakham University, Mahasarakham, Thailand; Department of Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand

Search for other papers by Penchom Janwan in
Current site
Google Scholar
PubMed Close
,
Tongjit Thanchomnang Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Faculty of Medicine, Mahasarakham University, Mahasarakham, Thailand; Department of Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand

Search for other papers by Tongjit Thanchomnang in
Current site
Google Scholar
PubMed Close
,
Kittisak Sawanyawisuth Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Faculty of Medicine, Mahasarakham University, Mahasarakham, Thailand; Department of Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand

Search for other papers by Kittisak Sawanyawisuth in
Current site
Google Scholar
PubMed Close
,
Nimit Morakote Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Faculty of Medicine, Mahasarakham University, Mahasarakham, Thailand; Department of Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand

Search for other papers by Nimit Morakote in
Current site
Google Scholar
PubMed Close
, and
Wanchai Maleewong Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Research and Diagnostic Center for Emerging Infectious Diseases, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Faculty of Medicine, Mahasarakham University, Mahasarakham, Thailand; Department of Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand; Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand

Search for other papers by Wanchai Maleewong in
Current site
Google Scholar
PubMed Close
DOI:
https://doi.org/10.4269/ajtmh.13-0113
Volume/Issue:
Volume 89: Issue 2
Page(s):
376–379
Restricted access
Get Permissions

Human trichostrongylosis has been reported in Thailand. Recent reports in Lao People's Democratic Republic concerning species identification urged us to investigate species distribution in Thailand. We report eight human cases in Thailand and Lao People's Democratic Republic that were found to be infected by Trichostrongylus colubriformis and T. axei identified and confirmed by molecular techniques. This evidence is the first molecular evidence of human T. colubriformis and T. axei infection in Thailand. Infection by these two species was apparently epidemic in these areas. It is necessary to proceed with more comprehensive veterinary and epidemiologic studies to enable the practical prevention and control of this parasitic zoonosis.

Author Notes

* Address correspondence to Pewpan M. Inatapn, Faculty of Medicine, Khon Kaen University, Khon Kaen 40002, Thailand. E-mail: pewpan@kku.ac.th

Financial support: This research was funded by grants from the National Science and Technology Development Agency (Discovery-Based Development Grant); the Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission, Thailand; and the Research and Diagnostic Center for Emerging Infectious Diseases and the Faculty of Medicine, Khon Kaen University. I.P. was supported by a Khon Kaen University Grant. P.M.I., T.T., and W.M. were supported by a Thailand Research Fund Senior Research Scholar Grant no. RTA5580004.

Authors' addresses: Issarapong Phosuk, Pewpan M. Intapan, Oranuch Sanpool, Penchom Janwan, and Wanchai Maleewong, Department of Parasitology, Faculty of Medicine and Research and Diagnostic Center for Emerging Infectious Diseases, Khon Kaen University, Khon Kaen, Thailand, E-mails: issarapong2oum@gmail.com, pewpan@kku.ac.th, sanpoolor@yahoo.com, pair_wu@yahoo.com, and wanch_ma@kku.ac.th. Tongjit Thanchomnang, Faculty of Medicine, Mahasarakham University, Mahasarakham, Thailand, E-mail: thanchomnang@yahoo.com. Kittisak Sawanyawisuth, Department of Medicine, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand, E-mail: kittisak@kku.ac.th. Nimit Morakote, Department of Parasitology, Faculty of Medicine, Chiang Mai University, Chiang Mai, Thailand, E-mail: nimit@med.cmu.ac.th.

Copyright:
© The American Society of Tropical Medicine and Hygiene 2013
Received:
01 Mar 2013
|
Accepted:
17 May 2013
|
Published Online:
07 Aug 2013
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
Online ISSN:
1476-1645
  • 1.

    Anderson RC, 2000. Nematode Parasites of Vertebrates: Their Development and Transmission, 2nd Ed. New York, NY: CABI.

  • 2.

    Tongson MS, Eduardo SL, 1982. Trichostrongylidiosis. Steel JH, Schultz MG, eds. CRC Handbook Series in Zoonoses. Section C: Parasitic Zoonoses, Vol II. Boca Raton, FL: CRC Press, 331–338.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 3.

    Lattes S, Ferte H, Delaunay P, Depaquit J, Vassallo M, Vittier M, Kokcha S, Coulibaly E, Marty P, 2011. Trichostrongylus colubriformis nematode infections in humans, France. Emerg Infect Dis 17: 1301–1302.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 4.

    Wall EC, Bhatnagar N, Watson J, Doherty T, 2011. An unusual case of hypereosinophilia and abdominal pain: an outbreak of Trichostrongylus imported from New Zealand. J Travel Med 18: 59–60.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 5.

    John DT, Petri WA, 2006. Markell and Voge's Medical Parasitology, 9th Ed. St. Louis, MO: Elsevier, 266–267.

  • 6.

    Ghadirian E, Arfaa F, 1975. Present status of trichostrongyliasis in Iran. Am J Trop Med Hyg 24: 935–941.

  • 7.

    Yong TS, Lee JH, Sim S, Lee J, Min DY, Chai JY, Eom KS, Sohn WM, Lee SH, Rim HJ, 2007. Differential diagnosis of Trichostrongylus and hookworm eggs via PCR using ITS-1 sequence. Korean J Parasitol 45: 69–74.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 8.

    Sato M, Yoonuan T, Sanguankiat S, Nuamtanong S, Pongvongsa T, Phimmayoi I, Phanhanan V, Boupha B, Moji K, Waikagul J, 2011. Human Trichostrongylus colubriformis infection in a rural village in Laos. Am J Trop Med Hyg 84: 52–54.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 9.

    Panasoponkul C, Radomyos P, Singhasivanon V, 1985. Trichostrongylus infection in a Thai boy. Southeast Asian J Trop Med Public Health 16: 513–514.

  • 10.

    Jariya P, Lertlaituan P, Junnoo V, Bedavanij A, Tesjaroen S, 1988. One human case of trichostrongyliasis in Thailand. J Med Assoc Thai 71: 469–470.

  • 11.

    Elkins DB, Haswell-Elkins M, Anderson RM, 1986. The epidemiology and control of intestinal helminths in the Pulicat Lake region of Southern India. I. Study design and pre-and post-treatment observations on Ascaris lumbricoides infection. Trans R Soc Trop Med Hyg 80: 774–792.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 12.

    Koga K, Kasuya S, Khamboonruang C, Sukhavat K, Ieda M, Takatsuka N, Kita K, Ohtomo H, 1991. A modified agar plate method for detection of Strongyloides stercoralis. Am J Trop Med Hyg 45: 518–521.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 13.

    Hoste H, Chilton NB, Beveridge I, Gasser RB, 1998. A comparison of the first internal transcribed spacer of ribosomal DNA in seven species of Trichostrongylus (Nematoda: Trichostrongylidae). Int J Parasitol 28: 1251–1260.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 14.

    Thompson JD, Higgins DG, Gibson TJ, 1994. CLUSTAL W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choice. Nucleic Acids Res 22: 4673–4680.

    • PubMed
    • Search Google Scholar
    • Export Citation
  • 15.

    Kumar S, Tamura K, Nei M, 2004. MEGA3: Integrated software for molecular evolutionary genetics analysis and sequence alignment. Brief Bioinform 5: 150–163.

Past two years Past Year Past 30 Days
Abstract Views 470 394 26
Full Text Views 389 17 2
PDF Downloads 152 17 2
 
 
 
 
Affiliate Membership Banner
 
 
Research for Health Information Banner
 
 
CLOCKSS
 
 
 
Society Publishers Coalition Banner
Save