ProCite
RefWorks
Reference Manager
BibTeX
Zotero
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-
1.
Pacheco RS, Brito CM, 1999. Reflections on the population dymanics of Trypanosoma cruzi: heterogeneity versus plasticity. Mem Inst Oswaldo Cruz 94 (Suppl I): 199– 201.
-
2.
Minning TA, Weatherly DB, Flibotte S, Tarleton R, 2011. Widespread focal copy number variations (CNV) and whole chromosome aneuploidies in Trypanosoma cruzi strains revealed by array comparative genomic hybridization. BMC Genomics 12: 139– 150.
-
3.
Rassi A, Luquetti A, 1992. Therapy of Chagas disease. Wendel S, Brener Z, Camargo ME, Rassi A, eds. Chagas Disease (American Trypanosomiasis): Its Impact on Transfusion and Clinical Medicine. São Paulo, Brazil: ISBT Brazil'92, 237– 247.
-
4.
León-Pérez F, Gómez-Garcia L, Alejandre-Aguilar R, López R, Monteón VM, 2007. Mexican Trypanosoma cruzi isolates: in vitro susceptibility of epimastigotes to anti-Trypanosoma cruzi drugs and metacyclic forms to complement-mediated lysis. Vector Borne Zoonotic Dis 7: 330– 336.
-
5.
Aguirre-Alvarado C, Zaragoza-Martínez F, Rodríguez-Paez L, Nogueda B, Baeza I, Wong C, 2007. In vitro and in vivo trypanocidal activity of the ethyl esters of N-allyl and N-propyl oxamates using different Trypanosoma cruzi strains. J Enzyme Inhib Med Chem 22: 227– 233.
-
6.
Urbina JA, 2010. Specific chemotherapy of Chagas disease: relevance, current limitations and new approaches. Acta Trop 115: 55– 68.
-
7.
Toledo MJ, Bahia MT, Carneiro CM, 2003. Chemotherapy with benznidazole and itraconazole for mice infected with different Trypanosoma cruzi clonal genotypes. Antimicrob Agents Chemother 47: 223– 230.
-
8.
Ruiz-Sanchez R, de Leon MP, Matta V, Reyes PA, Monteon V, 2005. Trypanosoma cruzi isolates from Mexican and Guatemalan acute and chronic chagasic cardiopathy patients belong to Trypanosoma cruzi I. Mem Inst Oswaldo Cruz 100: 281– 283.
-
9.
Brener Z, 1962. Therapeutic activity and criterion of cure on mice experimentally infected with Trypanosoma cruzi. Rev Inst Med Trop Sao Paulo 4: 389– 396.
-
10.
Degrave W, Fragoso S, Britto C, Van Heuverswyn H, Kidane G, Cardoso M, Mueller R, Simpson L, Morel C, 1988. Peculiar sequence organization of kinetoplast DNA minicircles from Trypanosoma cruzi. Mol Biochem Parasitol 27: 63– 70.
-
11.
Ballinas-Verdugo M, Reyes PA, Mejia-Dominguez A, López R, Matta V, Monteón VM, 2011. Enzyme-linked immunosorbent assay and polymerase chain reaction performance using Mexican and Guatemalan discrete typing unit I strains of Trypanosoma cruzi. Vector Borne Zoonotic Dis 12: 1569– 1575.
-
12.
Dzib Salazar D, Peña Hernández V, Ake Canche B, López R, Monteón V, 2009. Leukoreduction by centrifugation does not eliminate Trypanosma cruzi from infected blood units. Vector Borne Zoonotic Dis 3: 235– 241.
-
13.
Monteon V, Hernández O, López R, Reyes PA, 2009. Cytokine expression at the inoculation site and nearby tissue an animal model infected with metacyclic trypomastigotes of Trypanosoma cruzi. Trop Med Health 37: 141– 147.
-
14.
Gómez-Garcia L, Alejandre-Aguilar R, Aranda-Fraustro A, López R, Monteón VM, 2005. Description of inflammation and cytokine profile at the inoculation site and in heart tissue of mice re-infected with Trypanosoma cruzi vector derived-metacyclic trypomastigotes. Parasitology 130: 511– 522.
-
15.
Siqueira R, Andrade S, 2009. Trypanosoma cruzi: parasite antigens sequestered in heart interstitial dendritic cells are related to persisting mycarditis in benznidazole-treated mice. Mem Inst Oswaldo Cruz 107: 1023– 1030.
-
16.
Toledo MJ, Bahia MT, Veloso VM, Carneiro CM, Machado-Coelho G, Alves C, Martins H, Cruz R, Tafuri W, Lana M, 2004. Effects of specific treatment on parasitological and histopathological parameters in mice infected with different Trypanosoma cruzi clonal genotypes. J Antimicrob Chemother 53: 1045– 1053.
-
17.
Martins H, Figueiredo L, Valamiel-Silva J, Carneiro C, Machado-Coelho G, Vitelli-Avelar D, Bahia M, Martins-Filho O, Macedo A, Lana M, 2008. Persistence of PCR-positive tissue in beznidazole-treated mice with negative blood parasitological and serological tests in dual infections with Trypanosoma cruzi stocks from different genotypes. J Antimicrob Chemother 61: 1319– 1327.
| Past two years | Past Year | Past 30 Days | |
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| Abstract Views | 127 | 111 | 9 |
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Decreased Intensity of Inflammation in Benznidazole-Treated Mice Inoculated with Trypanosoma cruzi I Stocks from Mexico and Persistence of Circulating Parasites
Guillermo Cruz-Zetina
Guillermo Cruz-Zetina
Centro de Investigaciones Biomédicas e Centro de Ecologia, Pesquerias y Oceanográfia del Golfo de Mexico, Universidad Autónoma de Campeche, Campeche, Mexico; Laboratorio de Docencia, Investigación y Servicios, Inmunología y Biología Molecular Universidad Veracruzana, Orizaba, Mexico; Centro de Investigaciones Biomedicas, Universidad Veracruzana, Xalapa, Mexico
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Rodolfo del Rio-Rodriguez
Rodolfo del Rio-Rodriguez
Centro de Investigaciones Biomédicas e Centro de Ecologia, Pesquerias y Oceanográfia del Golfo de Mexico, Universidad Autónoma de Campeche, Campeche, Mexico; Laboratorio de Docencia, Investigación y Servicios, Inmunología y Biología Molecular Universidad Veracruzana, Orizaba, Mexico; Centro de Investigaciones Biomedicas, Universidad Veracruzana, Xalapa, Mexico
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Angel Ramos-Ligonio
Angel Ramos-Ligonio
Centro de Investigaciones Biomédicas e Centro de Ecologia, Pesquerias y Oceanográfia del Golfo de Mexico, Universidad Autónoma de Campeche, Campeche, Mexico; Laboratorio de Docencia, Investigación y Servicios, Inmunología y Biología Molecular Universidad Veracruzana, Orizaba, Mexico; Centro de Investigaciones Biomedicas, Universidad Veracruzana, Xalapa, Mexico
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Ruth López
Ruth López
Centro de Investigaciones Biomédicas e Centro de Ecologia, Pesquerias y Oceanográfia del Golfo de Mexico, Universidad Autónoma de Campeche, Campeche, Mexico; Laboratorio de Docencia, Investigación y Servicios, Inmunología y Biología Molecular Universidad Veracruzana, Orizaba, Mexico; Centro de Investigaciones Biomedicas, Universidad Veracruzana, Xalapa, Mexico
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Victor Monteon
Victor Monteon
Centro de Investigaciones Biomédicas e Centro de Ecologia, Pesquerias y Oceanográfia del Golfo de Mexico, Universidad Autónoma de Campeche, Campeche, Mexico; Laboratorio de Docencia, Investigación y Servicios, Inmunología y Biología Molecular Universidad Veracruzana, Orizaba, Mexico; Centro de Investigaciones Biomedicas, Universidad Veracruzana, Xalapa, Mexico
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We analyzed the intensity of inflammation and parasitism in BALB/c mice infected with Trypanosoma cruzi I stocks from Mexico with and without benznidazole treatment in the acute phase of disease. Heart and skeletal muscles were evaluated for parasites and inflammation and blood was evaluated for persistence of circulating parasites. Parasitemia was influenced by T. cruzi stocks used and benznidazole treatment. This treatment cleared circulating parasites three days after starting treatment when monitored by direct microscopy. There was a significant reduction of inflammation in skeletal muscles after benznidazole treatment in animals infected with Mexican T. cruzi I stocks (P < 0.05), but this reduction was not significant in the heart (P > 0.05). Trypanosoma cruzi I parasites from Mexico were demonstrated by polymerase chain reaction in tissues and blood of animals after benznidazole treatment.
Author Notes
Financial support: This study was supported by Consejo Nacional de Ciencia y Tecnología, Mexico.
Authors' addresses: Guillermo Cruz-Zetina, Ruth López, and Victor Monteon, Centro Investigaciones Biomedicas, Universidad Autonoma Campeche, Campeche, Mexico, E-mails: zetinaguillermo@gmail.com, dzinup@hotmail.com, and victormonteon@yahoo.com.mx. Rodolfo del Rio-Rodriguez, Centro de Ecologia, Pesquerias y Oceanográfia del Golfo de Mexico, Universidad Autonoma Campeche, Campeche, Mexico, E-mail: redelrio@uacam.mx. Angel Ramos-Ligonio, Universidad Veracruzana, Prolongación Oriente 6 No. 1009 Col. Rafael Alvarado CP 94340, Orizaba, Veracruz 94340, Mexico, E-mail: angramos@uv.mx.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Pacheco RS, Brito CM, 1999. Reflections on the population dymanics of Trypanosoma cruzi: heterogeneity versus plasticity. Mem Inst Oswaldo Cruz 94 (Suppl I): 199– 201.
-
2.
Minning TA, Weatherly DB, Flibotte S, Tarleton R, 2011. Widespread focal copy number variations (CNV) and whole chromosome aneuploidies in Trypanosoma cruzi strains revealed by array comparative genomic hybridization. BMC Genomics 12: 139– 150.
-
3.
Rassi A, Luquetti A, 1992. Therapy of Chagas disease. Wendel S, Brener Z, Camargo ME, Rassi A, eds. Chagas Disease (American Trypanosomiasis): Its Impact on Transfusion and Clinical Medicine. São Paulo, Brazil: ISBT Brazil'92, 237– 247.
-
4.
León-Pérez F, Gómez-Garcia L, Alejandre-Aguilar R, López R, Monteón VM, 2007. Mexican Trypanosoma cruzi isolates: in vitro susceptibility of epimastigotes to anti-Trypanosoma cruzi drugs and metacyclic forms to complement-mediated lysis. Vector Borne Zoonotic Dis 7: 330– 336.
-
5.
Aguirre-Alvarado C, Zaragoza-Martínez F, Rodríguez-Paez L, Nogueda B, Baeza I, Wong C, 2007. In vitro and in vivo trypanocidal activity of the ethyl esters of N-allyl and N-propyl oxamates using different Trypanosoma cruzi strains. J Enzyme Inhib Med Chem 22: 227– 233.
-
6.
Urbina JA, 2010. Specific chemotherapy of Chagas disease: relevance, current limitations and new approaches. Acta Trop 115: 55– 68.
-
7.
Toledo MJ, Bahia MT, Carneiro CM, 2003. Chemotherapy with benznidazole and itraconazole for mice infected with different Trypanosoma cruzi clonal genotypes. Antimicrob Agents Chemother 47: 223– 230.
-
8.
Ruiz-Sanchez R, de Leon MP, Matta V, Reyes PA, Monteon V, 2005. Trypanosoma cruzi isolates from Mexican and Guatemalan acute and chronic chagasic cardiopathy patients belong to Trypanosoma cruzi I. Mem Inst Oswaldo Cruz 100: 281– 283.
-
9.
Brener Z, 1962. Therapeutic activity and criterion of cure on mice experimentally infected with Trypanosoma cruzi. Rev Inst Med Trop Sao Paulo 4: 389– 396.
-
10.
Degrave W, Fragoso S, Britto C, Van Heuverswyn H, Kidane G, Cardoso M, Mueller R, Simpson L, Morel C, 1988. Peculiar sequence organization of kinetoplast DNA minicircles from Trypanosoma cruzi. Mol Biochem Parasitol 27: 63– 70.
-
11.
Ballinas-Verdugo M, Reyes PA, Mejia-Dominguez A, López R, Matta V, Monteón VM, 2011. Enzyme-linked immunosorbent assay and polymerase chain reaction performance using Mexican and Guatemalan discrete typing unit I strains of Trypanosoma cruzi. Vector Borne Zoonotic Dis 12: 1569– 1575.
-
12.
Dzib Salazar D, Peña Hernández V, Ake Canche B, López R, Monteón V, 2009. Leukoreduction by centrifugation does not eliminate Trypanosma cruzi from infected blood units. Vector Borne Zoonotic Dis 3: 235– 241.
-
13.
Monteon V, Hernández O, López R, Reyes PA, 2009. Cytokine expression at the inoculation site and nearby tissue an animal model infected with metacyclic trypomastigotes of Trypanosoma cruzi. Trop Med Health 37: 141– 147.
-
14.
Gómez-Garcia L, Alejandre-Aguilar R, Aranda-Fraustro A, López R, Monteón VM, 2005. Description of inflammation and cytokine profile at the inoculation site and in heart tissue of mice re-infected with Trypanosoma cruzi vector derived-metacyclic trypomastigotes. Parasitology 130: 511– 522.
-
15.
Siqueira R, Andrade S, 2009. Trypanosoma cruzi: parasite antigens sequestered in heart interstitial dendritic cells are related to persisting mycarditis in benznidazole-treated mice. Mem Inst Oswaldo Cruz 107: 1023– 1030.
-
16.
Toledo MJ, Bahia MT, Veloso VM, Carneiro CM, Machado-Coelho G, Alves C, Martins H, Cruz R, Tafuri W, Lana M, 2004. Effects of specific treatment on parasitological and histopathological parameters in mice infected with different Trypanosoma cruzi clonal genotypes. J Antimicrob Chemother 53: 1045– 1053.
-
17.
Martins H, Figueiredo L, Valamiel-Silva J, Carneiro C, Machado-Coelho G, Vitelli-Avelar D, Bahia M, Martins-Filho O, Macedo A, Lana M, 2008. Persistence of PCR-positive tissue in beznidazole-treated mice with negative blood parasitological and serological tests in dual infections with Trypanosoma cruzi stocks from different genotypes. J Antimicrob Chemother 61: 1319– 1327.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 127 | 111 | 9 |
| Full Text Views | 222 | 5 | 0 |
| PDF Downloads | 52 | 2 | 0 |