Dengue-3 Outbreak in Paraguay: Investigations Using Capillary Blood Samples on Filter Paper

Severine Matheus Centre National de Référence des Arbovirus, Institut Pasteur de la Guyane, Cayenne, French Guiana; Unité d’épidemiologie, Institut Pasteur de la Guyane, Cayenne, French Guiana; Unité d’entomologie Institut Pasteur de la Guyane, Cayenne, French Guiana; Laboratoire des Interactions Virus Hôtes, Institut Pasteur de la Guyane, Cayenne, French Guiana; Institut de Médecine Tropicale du Service de Santé des Armées, Le Pharo, Marseille, France

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Jean-Baptiste Meynard Centre National de Référence des Arbovirus, Institut Pasteur de la Guyane, Cayenne, French Guiana; Unité d’épidemiologie, Institut Pasteur de la Guyane, Cayenne, French Guiana; Unité d’entomologie Institut Pasteur de la Guyane, Cayenne, French Guiana; Laboratoire des Interactions Virus Hôtes, Institut Pasteur de la Guyane, Cayenne, French Guiana; Institut de Médecine Tropicale du Service de Santé des Armées, Le Pharo, Marseille, France

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Anne Lavergne Centre National de Référence des Arbovirus, Institut Pasteur de la Guyane, Cayenne, French Guiana; Unité d’épidemiologie, Institut Pasteur de la Guyane, Cayenne, French Guiana; Unité d’entomologie Institut Pasteur de la Guyane, Cayenne, French Guiana; Laboratoire des Interactions Virus Hôtes, Institut Pasteur de la Guyane, Cayenne, French Guiana; Institut de Médecine Tropicale du Service de Santé des Armées, Le Pharo, Marseille, France

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Romain Girod Centre National de Référence des Arbovirus, Institut Pasteur de la Guyane, Cayenne, French Guiana; Unité d’épidemiologie, Institut Pasteur de la Guyane, Cayenne, French Guiana; Unité d’entomologie Institut Pasteur de la Guyane, Cayenne, French Guiana; Laboratoire des Interactions Virus Hôtes, Institut Pasteur de la Guyane, Cayenne, French Guiana; Institut de Médecine Tropicale du Service de Santé des Armées, Le Pharo, Marseille, France

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David Moua Centre National de Référence des Arbovirus, Institut Pasteur de la Guyane, Cayenne, French Guiana; Unité d’épidemiologie, Institut Pasteur de la Guyane, Cayenne, French Guiana; Unité d’entomologie Institut Pasteur de la Guyane, Cayenne, French Guiana; Laboratoire des Interactions Virus Hôtes, Institut Pasteur de la Guyane, Cayenne, French Guiana; Institut de Médecine Tropicale du Service de Santé des Armées, Le Pharo, Marseille, France

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Bhety Labeau Centre National de Référence des Arbovirus, Institut Pasteur de la Guyane, Cayenne, French Guiana; Unité d’épidemiologie, Institut Pasteur de la Guyane, Cayenne, French Guiana; Unité d’entomologie Institut Pasteur de la Guyane, Cayenne, French Guiana; Laboratoire des Interactions Virus Hôtes, Institut Pasteur de la Guyane, Cayenne, French Guiana; Institut de Médecine Tropicale du Service de Santé des Armées, Le Pharo, Marseille, France

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Philippe Dussart Centre National de Référence des Arbovirus, Institut Pasteur de la Guyane, Cayenne, French Guiana; Unité d’épidemiologie, Institut Pasteur de la Guyane, Cayenne, French Guiana; Unité d’entomologie Institut Pasteur de la Guyane, Cayenne, French Guiana; Laboratoire des Interactions Virus Hôtes, Institut Pasteur de la Guyane, Cayenne, French Guiana; Institut de Médecine Tropicale du Service de Santé des Armées, Le Pharo, Marseille, France

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Vincent Lacoste Centre National de Référence des Arbovirus, Institut Pasteur de la Guyane, Cayenne, French Guiana; Unité d’épidemiologie, Institut Pasteur de la Guyane, Cayenne, French Guiana; Unité d’entomologie Institut Pasteur de la Guyane, Cayenne, French Guiana; Laboratoire des Interactions Virus Hôtes, Institut Pasteur de la Guyane, Cayenne, French Guiana; Institut de Médecine Tropicale du Service de Santé des Armées, Le Pharo, Marseille, France

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Xavier Deparis Centre National de Référence des Arbovirus, Institut Pasteur de la Guyane, Cayenne, French Guiana; Unité d’épidemiologie, Institut Pasteur de la Guyane, Cayenne, French Guiana; Unité d’entomologie Institut Pasteur de la Guyane, Cayenne, French Guiana; Laboratoire des Interactions Virus Hôtes, Institut Pasteur de la Guyane, Cayenne, French Guiana; Institut de Médecine Tropicale du Service de Santé des Armées, Le Pharo, Marseille, France

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During a dengue-3 outbreak in Paraguay at the beginning of 2007, capillary blood samples absorbed onto filter papers were collected from 44 suspected cases. These samples were subjected to three molecular and serologic tests, and 31 of the 44 samples gave a positive result by at least one of the techniques used. Molecular analyses detected the dengue-3 serotype in 22 patients and additionally the dengue-2 serotype in two patients. Therefore two different serotypes were co-circulating during this outbreak. Overall, this study validates the use of dried-blood samples for field screening investigations. Indeed, all types of laboratory studies of dengue were possible with samples consisting of a few drops of dried blood from finger pricks.

Author Notes

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