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RefWorks
Reference Manager
BibTeX
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-
1
Desjeux P, Alvar J, 2003. Leishmania/HIV co-infections: epidemiology in Europe. Ann Trop Med Parasitol 97 :3–15.
-
2
Alvar J, Cañavate C, Gutierrez-Solar B, Jiménez M, Laguna F, López-Vélez R, Molina R, Moreno J, 1997. Leishmania and human immunodeficiency virus coinfection: the first 10 years. Clin Microbiol Rev 10 :298–319.
-
3
Smyth AJ, Ghosh A, Hassan MQ, Basu D, De Bruijn MH, Adhya S, Mallik KK, Barker DC, 1992. Rapid and sensitive detection of Leishmania kinetoplast DNA from spleen and blood samples of kalaazar patients. Parasitology 105 :183–192.
-
4
Pizzuto M, Piazza M, Senese D, Scalamogna C, Calattini S, Corsico L, Persico T, Adriani B, Magni C, Guaraldi G, Gaiera G, Ludovisi A, Gramiccia M, Galli M, Moroni M, Corbellino M, Antinori S, 2001. Role of PCR in diagnosis and prognosis of visceral leishmaniasis in patients coinfected with human immunodeficiency virus type 1. J Clin Microbiol 39 :357–361.
-
5
Fisa R, Riera C, Ribera E, Gállego M, Portús M, 2002. A nested polymerase chain reaction for diagnosis and follow-up of human visceral leishmaniasis patients using blood samples. Trans R Soc Trop Med Hyg 96 :S191–S194.
-
6
Mebrahtu YB, Hendricks LD, Oster CN, Lawyer PG, Perkins PV, Pamba H, Koech D, Roberts CR, 1993. Leishmania donovani parasites in the nasal secretions, tonsillopharyngeal mucosa, and urine centrifugates of visceral leishmaniasis patients in Kenya. Am J Trop Med Hyg 48 :530–535.
-
7
Riera C, Valladares JE, 1996. Viable Leishmania infantum in urine and semen in experimentally infected dogs. Parasitol Today 12 :412.
-
8
Solano-Gallego L, Rodriguez-Cortes A, Trotta M, Zampieron C, Razia L, Furlanello T, Caldin M, Roura X, Alberola J, 2007. Detection of Leishmania infantum DNA by fret-based real-time PCR in urine from dogs with natural clinical leishmaniosis. Vet Parasitol 147 :315–319.
-
9
De Colmenares M, Portús M, Riera C, Gállego M, Aisa MJ, Torras S, Muñoz C, 1995. Short report: detection of 72–75-kD and 123-kD fractions of Leishmania antigen in urine of patients with visceral leishmaniasis. Am J Trop Med Hyg 52 :427–428.
-
10
Kohanteb J, Ardehali SM, Rezai HR, 1987. Detection of Leishmania donovani soluble antigen and antibody in the urine of visceral leishmaniasis patients. Trans R Soc Trop Med Hyg 81 :578–580.
-
11
Attar ZJ, Chance ML, el-Safi S, Carney J, Azazy A, El-Hadi M, Dourado C, Hommel M, 2001. Latex agglutination test for the detection of urinary antigens in visceral leishmaniasis. Acta Trop 78 :11–16.
-
12
Riera C, Fisa R, López-Chejade P, Ribera E, Carrió J, Falcó V, Molina I, Gállego M, Portús M, 2004. Evaluation of a latex agglutination test (KAtex) for detection of Leishmania antigen in urine of patients with HIV-Leishmania coinfection: value in diagnosis and post-treatment follow-up. Eur J Clin Microbiol Infect Dis 23 :899–904.
-
13
Riera C, Fisa R, Ribera E, Carrió J, Falcó V, Gállego M, Moner L, Molina I, Portús M, 2005. Value of culture and nested polymerase chain reaction of blood in the prediction of relapses in patients co-infected with Leishmania and human immunodeficiency virus. Am J Trop Med Hyg 73 :1012–1015.
-
14
Giovannelli L, Lama A, Capra G, Giordano V, Arico P, Ammatuna P, 2004. Detection of human papillomavirus DNA in cervical samples: analysis of the new PGMY-PCR compared to the hybrid capture II and MY-PCR assays and a two-step nested PCR assay. J Clin Microbiol 42 :3861–3864.
-
15
Cohen J, 1960. A coefficient of agreement for nominal scales. Educ Psychol Meas 20 :37–46.
-
16
Lachaud L, Dereure J, Chabbert E, Reynes J, Mauboussin JM, Oziol E, Dedet JP, Bastien P, 2000. Optimized PCR using patient blood samples for diagnosis and follow-up of visceral leishmaniasis, with special reference to AIDS patients. J Clin Microbiol 38 :236–240.
-
17
Bossolasco S, Gaiera G, Olchini D, Gulletta M, Martello L, Bestetti A, Bossi L, Germagnoli L, Lazzarin A, Uberti-Foppa C, Cinque P, 2003. Real-time PCR assay for clinical management of human immunodeficiency virus-infected patients with visceral leishmaniasis. J Clin Microbiol 41: 5080–5084. Erratum in J Clin Microbiol 42 :1858.
-
18
Fisa R, Riera C, Gállego M, Manubens J, Portús M, 2001. Nested PCR for diagnosis of canine leishmaniosis in peripheral blood, lymph node and bone marrow aspirates. Vet Parasitol 99 :105–111.
-
19
Caravaca F, Munoz A, Pizarro JL, Saez de Santamaria J, Fernandez-Alonso J, 1991. Acute renal failure in visceral leishmaniasis. Am J Nephrol 11 :350–352.
-
20
Dutra M, Martinelli R, de Carvalho EM, Rodrigues LE, Brito E, Rocha H, 1985. Renal involvement in visceral leishmaniasis. Am J Kidney Dis 6 :22–27.
-
21
Salgado Filho N, Ferreira TM, Costa JM, 2003. [Involvement of the renal function in patients with visceral leishmaniasis (kalaazar)]. Rev Soc Bras Med Trop 36 :217–221.
-
22
Solano-Gallego L, Rodriguez A, Iniesta L, Arboix M, Portús M, Alberola J, 2003. Detection of anti-Leishmania immunoglobulin G antibodies in urine specimens of dogs with leishmaniasis. Clin Diagn Lab Immunol 10 :849–855.
-
23
Lichtenstein AV, Melkonyan HS, Tomei LD, Umansky SR, 2006. Novel applications of polymerase chain reaction to urinary nucleic acid analysis. Methods Mol Bio 336 :145–154.
-
24
Umansky SR, Tomei LD, 2006. Transrenal DNA testing: progress and perspectives. Expert Rev Mol Diagn 6 :153–163.
-
25
Mary C, Faraut F, Lascombe L, Dumon H, 2004. Quantification of Leishmania infantum DNA by a real-time PCR assay with high sensitivity. J Clin Microbiol 42 :5249–5255.
-
26
Riera C, Fisa R, Udina M, Gállego M, Portús M, 2004. Detection of Leishmania infantum cryptic infection in asymptomatic blood donors living in an endemic area (Eivissa, Balearic Islands, Spain) by different diagnostic methods. Trans R Soc Trop Med Hyg 98 :102–110.
-
27
Le Fichoux Y, Quaranta JF, Aufeuvre JP, Lelievre A, Marty P, Suffia I, Rousseau D, Kubar J, 1999. Ocurrence of Leishmania infantum parasitemia in asymptomatic blood donors living in an area of endemicity in Southern France. J Clin Microbiol 37 :1953–1957.
-
28
Lemesre L, Darcy F, Kweider M, Capron A, Santero F, 1988. Requirements of defined cultivation conditions for standard growth of Leishmania promastigotes in vitro.Acta Trop 45 :99–108.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 260 | 235 | 22 |
| Full Text Views | 236 | 3 | 1 |
| PDF Downloads | 57 | 3 | 1 |
Leishmania infantum DNA Detection in Urine from Patients with Visceral Leishmaniasis and after Treatment Control
Roser Fisa
Roser Fisa
Laboratory of Parasitology, Universitat de Barcelona, Barcelona, Spain; Infectious Diseases Department, Hospital Universitari Vall d‘Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain
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Cristina Riera
Cristina Riera
Laboratory of Parasitology, Universitat de Barcelona, Barcelona, Spain; Infectious Diseases Department, Hospital Universitari Vall d‘Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain
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Paulo López-Chejade
Paulo López-Chejade
Laboratory of Parasitology, Universitat de Barcelona, Barcelona, Spain; Infectious Diseases Department, Hospital Universitari Vall d‘Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain
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Israel Molina
Israel Molina
Laboratory of Parasitology, Universitat de Barcelona, Barcelona, Spain; Infectious Diseases Department, Hospital Universitari Vall d‘Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain
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Montserrat Gállego
Montserrat Gállego
Laboratory of Parasitology, Universitat de Barcelona, Barcelona, Spain; Infectious Diseases Department, Hospital Universitari Vall d‘Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain
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Vicenç Falcó
Vicenç Falcó
Laboratory of Parasitology, Universitat de Barcelona, Barcelona, Spain; Infectious Diseases Department, Hospital Universitari Vall d‘Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain
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Esteban Ribera
Esteban Ribera
Laboratory of Parasitology, Universitat de Barcelona, Barcelona, Spain; Infectious Diseases Department, Hospital Universitari Vall d‘Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain
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Montserrat Portús
Montserrat Portús
Laboratory of Parasitology, Universitat de Barcelona, Barcelona, Spain; Infectious Diseases Department, Hospital Universitari Vall d‘Hebron, Universitat Autònoma de Barcelona, Barcelona, Spain
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A urine–polymerase chain reaction (PCR) assay was validated for diagnosis of human visceral leishmaniosis (VL), taking advantage of the accessibility of urine samples. Leishmania infantum DNA presence was examined in 17 urine samples from 17 patients with VL during a clinical episode and in 55 urine samples from 17 patients with VL monitored after treatment at different intervals. Fifty-nine urine samples from 59 controls with no history of VL were also studied. The urine–PCR test was positive in 15/17 samples obtained during the episode (sensitivity, 88%). None of the controls tested were urine–PCR positive (specificity, 100%). During the monitoring period, 25% of the samples gave a positive urine–PCR. Results were compared with other diagnostic methods, such as urine antigen detection and peripheral blood–PCR and culture, with good concordance during the clinical episode and differences in the follow-up period. This study suggests that urine–PCR is sensitive for diagnosis and may be useful to monitor treatment efficacy.
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-
1
Desjeux P, Alvar J, 2003. Leishmania/HIV co-infections: epidemiology in Europe. Ann Trop Med Parasitol 97 :3–15.
-
2
Alvar J, Cañavate C, Gutierrez-Solar B, Jiménez M, Laguna F, López-Vélez R, Molina R, Moreno J, 1997. Leishmania and human immunodeficiency virus coinfection: the first 10 years. Clin Microbiol Rev 10 :298–319.
-
3
Smyth AJ, Ghosh A, Hassan MQ, Basu D, De Bruijn MH, Adhya S, Mallik KK, Barker DC, 1992. Rapid and sensitive detection of Leishmania kinetoplast DNA from spleen and blood samples of kalaazar patients. Parasitology 105 :183–192.
-
4
Pizzuto M, Piazza M, Senese D, Scalamogna C, Calattini S, Corsico L, Persico T, Adriani B, Magni C, Guaraldi G, Gaiera G, Ludovisi A, Gramiccia M, Galli M, Moroni M, Corbellino M, Antinori S, 2001. Role of PCR in diagnosis and prognosis of visceral leishmaniasis in patients coinfected with human immunodeficiency virus type 1. J Clin Microbiol 39 :357–361.
-
5
Fisa R, Riera C, Ribera E, Gállego M, Portús M, 2002. A nested polymerase chain reaction for diagnosis and follow-up of human visceral leishmaniasis patients using blood samples. Trans R Soc Trop Med Hyg 96 :S191–S194.
-
6
Mebrahtu YB, Hendricks LD, Oster CN, Lawyer PG, Perkins PV, Pamba H, Koech D, Roberts CR, 1993. Leishmania donovani parasites in the nasal secretions, tonsillopharyngeal mucosa, and urine centrifugates of visceral leishmaniasis patients in Kenya. Am J Trop Med Hyg 48 :530–535.
-
7
Riera C, Valladares JE, 1996. Viable Leishmania infantum in urine and semen in experimentally infected dogs. Parasitol Today 12 :412.
-
8
Solano-Gallego L, Rodriguez-Cortes A, Trotta M, Zampieron C, Razia L, Furlanello T, Caldin M, Roura X, Alberola J, 2007. Detection of Leishmania infantum DNA by fret-based real-time PCR in urine from dogs with natural clinical leishmaniosis. Vet Parasitol 147 :315–319.
-
9
De Colmenares M, Portús M, Riera C, Gállego M, Aisa MJ, Torras S, Muñoz C, 1995. Short report: detection of 72–75-kD and 123-kD fractions of Leishmania antigen in urine of patients with visceral leishmaniasis. Am J Trop Med Hyg 52 :427–428.
-
10
Kohanteb J, Ardehali SM, Rezai HR, 1987. Detection of Leishmania donovani soluble antigen and antibody in the urine of visceral leishmaniasis patients. Trans R Soc Trop Med Hyg 81 :578–580.
-
11
Attar ZJ, Chance ML, el-Safi S, Carney J, Azazy A, El-Hadi M, Dourado C, Hommel M, 2001. Latex agglutination test for the detection of urinary antigens in visceral leishmaniasis. Acta Trop 78 :11–16.
-
12
Riera C, Fisa R, López-Chejade P, Ribera E, Carrió J, Falcó V, Molina I, Gállego M, Portús M, 2004. Evaluation of a latex agglutination test (KAtex) for detection of Leishmania antigen in urine of patients with HIV-Leishmania coinfection: value in diagnosis and post-treatment follow-up. Eur J Clin Microbiol Infect Dis 23 :899–904.
-
13
Riera C, Fisa R, Ribera E, Carrió J, Falcó V, Gállego M, Moner L, Molina I, Portús M, 2005. Value of culture and nested polymerase chain reaction of blood in the prediction of relapses in patients co-infected with Leishmania and human immunodeficiency virus. Am J Trop Med Hyg 73 :1012–1015.
-
14
Giovannelli L, Lama A, Capra G, Giordano V, Arico P, Ammatuna P, 2004. Detection of human papillomavirus DNA in cervical samples: analysis of the new PGMY-PCR compared to the hybrid capture II and MY-PCR assays and a two-step nested PCR assay. J Clin Microbiol 42 :3861–3864.
-
15
Cohen J, 1960. A coefficient of agreement for nominal scales. Educ Psychol Meas 20 :37–46.
-
16
Lachaud L, Dereure J, Chabbert E, Reynes J, Mauboussin JM, Oziol E, Dedet JP, Bastien P, 2000. Optimized PCR using patient blood samples for diagnosis and follow-up of visceral leishmaniasis, with special reference to AIDS patients. J Clin Microbiol 38 :236–240.
-
17
Bossolasco S, Gaiera G, Olchini D, Gulletta M, Martello L, Bestetti A, Bossi L, Germagnoli L, Lazzarin A, Uberti-Foppa C, Cinque P, 2003. Real-time PCR assay for clinical management of human immunodeficiency virus-infected patients with visceral leishmaniasis. J Clin Microbiol 41: 5080–5084. Erratum in J Clin Microbiol 42 :1858.
-
18
Fisa R, Riera C, Gállego M, Manubens J, Portús M, 2001. Nested PCR for diagnosis of canine leishmaniosis in peripheral blood, lymph node and bone marrow aspirates. Vet Parasitol 99 :105–111.
-
19
Caravaca F, Munoz A, Pizarro JL, Saez de Santamaria J, Fernandez-Alonso J, 1991. Acute renal failure in visceral leishmaniasis. Am J Nephrol 11 :350–352.
-
20
Dutra M, Martinelli R, de Carvalho EM, Rodrigues LE, Brito E, Rocha H, 1985. Renal involvement in visceral leishmaniasis. Am J Kidney Dis 6 :22–27.
-
21
Salgado Filho N, Ferreira TM, Costa JM, 2003. [Involvement of the renal function in patients with visceral leishmaniasis (kalaazar)]. Rev Soc Bras Med Trop 36 :217–221.
-
22
Solano-Gallego L, Rodriguez A, Iniesta L, Arboix M, Portús M, Alberola J, 2003. Detection of anti-Leishmania immunoglobulin G antibodies in urine specimens of dogs with leishmaniasis. Clin Diagn Lab Immunol 10 :849–855.
-
23
Lichtenstein AV, Melkonyan HS, Tomei LD, Umansky SR, 2006. Novel applications of polymerase chain reaction to urinary nucleic acid analysis. Methods Mol Bio 336 :145–154.
-
24
Umansky SR, Tomei LD, 2006. Transrenal DNA testing: progress and perspectives. Expert Rev Mol Diagn 6 :153–163.
-
25
Mary C, Faraut F, Lascombe L, Dumon H, 2004. Quantification of Leishmania infantum DNA by a real-time PCR assay with high sensitivity. J Clin Microbiol 42 :5249–5255.
-
26
Riera C, Fisa R, Udina M, Gállego M, Portús M, 2004. Detection of Leishmania infantum cryptic infection in asymptomatic blood donors living in an endemic area (Eivissa, Balearic Islands, Spain) by different diagnostic methods. Trans R Soc Trop Med Hyg 98 :102–110.
-
27
Le Fichoux Y, Quaranta JF, Aufeuvre JP, Lelievre A, Marty P, Suffia I, Rousseau D, Kubar J, 1999. Ocurrence of Leishmania infantum parasitemia in asymptomatic blood donors living in an area of endemicity in Southern France. J Clin Microbiol 37 :1953–1957.
-
28
Lemesre L, Darcy F, Kweider M, Capron A, Santero F, 1988. Requirements of defined cultivation conditions for standard growth of Leishmania promastigotes in vitro.Acta Trop 45 :99–108.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 260 | 235 | 22 |
| Full Text Views | 236 | 3 | 1 |
| PDF Downloads | 57 | 3 | 1 |