- INTRODUCTION
- MATERIALS AND METHODS
- Samples.
- Detection of total IgM in cord blood serum samples.
- Samples collected during the breast-feeding period and after parturition.
- Schistosoma mansoni soluble egg antigen.
- Sodium dodecyl sulfate–polyacrylamide gel electrophoresis.
- Gel electroelution.
- Western blotting.
- Measurement of human schistosome-specific antibody response using an ELISA.
- Dot-ELISA.
- Statistical analysis.
- RESULTS
- Detection of Schistosoma antigen in serum and urine of infected pregnant women and controls.
- Schistosoma-specific antibody response.
- Detection of Schistosoma antigen and specific antibody in cord blood.
- Detection of Schistosoma antigen in breast milk and in urine of young children during the breast-feeding period.
- Detection of Schistosoma antigen in infants after parturition.
- DISCUSSION
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1
Capron A, Capron M, Dombrowicz D, Riveau G, 2001. Vaccine strategies against schistosomiasis: from concepts to clinical trials. Int Arch Allergy Immunol 124 :9–15.
-
2
Krugman S, 1975. Vertical transmission of hepatitis B and breast-feeding (letter). Lancet 2 :916.
-
3
Siegrist C-A, 2001. Neonatal and early life vaccinology. Vaccine 19 :3331–3346.
-
4
Eloi-Santos SM, Novato-Silva E, Maselli VM, Gazzinelli G, Colley DG, Correa-Oliveira R, 1989. Idiotypic sensitization in utero of children born to mothers with schistosomiasis or Chagas’ disease. J Clin Invest 84 :1028–1031.
-
5
Novato-Silva E, Gazzinelli G, Colley DG, 1992. Immune responses during human schistosomiasis mansoni. XVIII. Immunologic status of pregnant women and their neonates. Scand J Immunol 35 :429–437.
-
6
Attallah AM, Yones E, Ismail H, El Masry S, Tabll A, Elenein AA, El-Ghawalby NA, 1999. Immunochemical characterization and diagnostic potential of a 63-kilodalton Schistosoma antigen. Am J Trop Med Hyg 60 :493–497.
-
7
Attallah AM, Ismail H, El-Masry SA, Rizk H, El-Bendary M, Tabll A, Ezzat F, 1999. Rapid detection of a Schistosoma mansoni circulating antigen excreted in urine of infected individuals by using monoclonal antibody. J Clin Microbiol 37 :354–357.
-
8
Martin L, Beaver P, 1968. Evaluation of Kato thick smear technique for quantitative diagnosis of helminth infection. Am J Trop Med Hyg 17 :382–388.
-
9
da Silva LC, Ferri RC, 1968. Schistosoma mansoni homogenate active immunization of mice. Am J Trop Med Hyg 17 :367–371.
-
10
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ, 1951. Protein measurement with the folin phenol reagent. J Biol Chem 193 :265–275.
-
11
Laemmli UK, 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 :680–685.
-
12
Towbin H, Staehlin T, Gordon J, 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76 :4350–4354.
-
13
Silverstein AM, Segal S, 1975. The ontogeny of antigen-specific T cells. J Exp Med 142 :802–804.
-
14
Montesano MA, Colley DG, Eloi-Santos S, Freeman GL Jr, Secor WE, 1999. Neonatal idiotypic exposure alters subsequent cytokine, pathology, and survival patterns in experimental Schistosoma mansoni infections. J Exp Med 189 :637–645.
-
15
Carlier Y, Nzayimana H, Bout D, Capron A, 1980. Evaluation of circulating antigens by sandwich radioimmunoassay and of the antibodies and immune complexes in Schistosoma mansoni infected Africans parturants and their newborn children. Am J Trop Med Hyg 29 :74–81.
-
16
Romia SA, Handoussa AE, Youseff SA, el Zayat MM, 1992. Transplacental transfer of schistosomal antigens and antibodies. J Egypt Soc Parasitol 22 :575–582.
-
17
Hassan MM, Hassounah OA, Hegab M, Salah K, el-Mahrouky L, Galal N, 1997. Transmission of circulating schistosomal antigens from infected mothers to their newborns. J Egypt Soc Parasitol 27 :773–780.
-
18
Rueff-Juy D, Faure M, Drapier A, Cazenave P, 1999. Role of maternal Ig in the induction of C kappa-specific CD8+ T cell tolerance. J Immunol 161 :721–728.
-
19
Ichino M, Mor G, Conover J, Weiss WR, Takeno M, Ishii KJ, Klinman DM, 1999. Factors associated with the development of neonatal tolerance after the administration of a plasmid DNA vaccine. J Immunol 162 :3814–3818.
-
20
Schwartz RH, 1989. Acquisition of immunologic self-tolerance. Cell 57 :1073–1081.
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PLACENTAL AND ORAL DELIVERY OF SCHISTOSOMA MANSONI ANTIGEN FROM INFECTED MOTHERS TO THEIR NEWBORNS AND CHILDREN
ABDELFATTAH M. ATTALLAH
ABDELFATTAH M. ATTALLAH
Biotechnology Research Center, New Damietta City, Egypt
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GAMAL E. GHANEM
GAMAL E. GHANEM
Biotechnology Research Center, New Damietta City, Egypt
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HISHAM ISMAIL
HISHAM ISMAIL
Biotechnology Research Center, New Damietta City, Egypt
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AHMED M. EL WASEEF
AHMED M. EL WASEEF
Biotechnology Research Center, New Damietta City, Egypt
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A 63-kD Schistosoma mansoni antigen was detected in 149 (86%) of 174 umbilical cord blood sera from infected women at delivery. Specific IgG antibodies to this antigen were also detected in 80% of cord blood sera. The 63-kD antigen showed the same molecular mass by Western blotting when isolated from cord blood, maternal blood, breast milk, and urine from women infected with S. mansoni. This antigen was detected in the urine of 25 infants born to infected mothers and followed for 18–24 months after delivery. It was also detected in some infants up to 21 days after parturition and then disappeared at 28 days, demonstrating the influence of breast-feeding on persistence of antigen in infants born to infected women. Thus, exposure to Schistosoma antigens and maternal antibodies to this organism may influence the developing immune responses to natural infection or vaccination of children born in endemic areas.
Author Notes
- INTRODUCTION
- MATERIALS AND METHODS
- Samples.
- Detection of total IgM in cord blood serum samples.
- Samples collected during the breast-feeding period and after parturition.
- Schistosoma mansoni soluble egg antigen.
- Sodium dodecyl sulfate–polyacrylamide gel electrophoresis.
- Gel electroelution.
- Western blotting.
- Measurement of human schistosome-specific antibody response using an ELISA.
- Dot-ELISA.
- Statistical analysis.
- RESULTS
- Detection of Schistosoma antigen in serum and urine of infected pregnant women and controls.
- Schistosoma-specific antibody response.
- Detection of Schistosoma antigen and specific antibody in cord blood.
- Detection of Schistosoma antigen in breast milk and in urine of young children during the breast-feeding period.
- Detection of Schistosoma antigen in infants after parturition.
- DISCUSSION
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1
Capron A, Capron M, Dombrowicz D, Riveau G, 2001. Vaccine strategies against schistosomiasis: from concepts to clinical trials. Int Arch Allergy Immunol 124 :9–15.
-
2
Krugman S, 1975. Vertical transmission of hepatitis B and breast-feeding (letter). Lancet 2 :916.
-
3
Siegrist C-A, 2001. Neonatal and early life vaccinology. Vaccine 19 :3331–3346.
-
4
Eloi-Santos SM, Novato-Silva E, Maselli VM, Gazzinelli G, Colley DG, Correa-Oliveira R, 1989. Idiotypic sensitization in utero of children born to mothers with schistosomiasis or Chagas’ disease. J Clin Invest 84 :1028–1031.
-
5
Novato-Silva E, Gazzinelli G, Colley DG, 1992. Immune responses during human schistosomiasis mansoni. XVIII. Immunologic status of pregnant women and their neonates. Scand J Immunol 35 :429–437.
-
6
Attallah AM, Yones E, Ismail H, El Masry S, Tabll A, Elenein AA, El-Ghawalby NA, 1999. Immunochemical characterization and diagnostic potential of a 63-kilodalton Schistosoma antigen. Am J Trop Med Hyg 60 :493–497.
-
7
Attallah AM, Ismail H, El-Masry SA, Rizk H, El-Bendary M, Tabll A, Ezzat F, 1999. Rapid detection of a Schistosoma mansoni circulating antigen excreted in urine of infected individuals by using monoclonal antibody. J Clin Microbiol 37 :354–357.
-
8
Martin L, Beaver P, 1968. Evaluation of Kato thick smear technique for quantitative diagnosis of helminth infection. Am J Trop Med Hyg 17 :382–388.
-
9
da Silva LC, Ferri RC, 1968. Schistosoma mansoni homogenate active immunization of mice. Am J Trop Med Hyg 17 :367–371.
-
10
Lowry OH, Rosebrough NJ, Farr AL, Randall RJ, 1951. Protein measurement with the folin phenol reagent. J Biol Chem 193 :265–275.
-
11
Laemmli UK, 1970. Cleavage of structural proteins during the assembly of the head of bacteriophage T4. Nature 227 :680–685.
-
12
Towbin H, Staehlin T, Gordon J, 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications. Proc Natl Acad Sci USA 76 :4350–4354.
-
13
Silverstein AM, Segal S, 1975. The ontogeny of antigen-specific T cells. J Exp Med 142 :802–804.
-
14
Montesano MA, Colley DG, Eloi-Santos S, Freeman GL Jr, Secor WE, 1999. Neonatal idiotypic exposure alters subsequent cytokine, pathology, and survival patterns in experimental Schistosoma mansoni infections. J Exp Med 189 :637–645.
-
15
Carlier Y, Nzayimana H, Bout D, Capron A, 1980. Evaluation of circulating antigens by sandwich radioimmunoassay and of the antibodies and immune complexes in Schistosoma mansoni infected Africans parturants and their newborn children. Am J Trop Med Hyg 29 :74–81.
-
16
Romia SA, Handoussa AE, Youseff SA, el Zayat MM, 1992. Transplacental transfer of schistosomal antigens and antibodies. J Egypt Soc Parasitol 22 :575–582.
-
17
Hassan MM, Hassounah OA, Hegab M, Salah K, el-Mahrouky L, Galal N, 1997. Transmission of circulating schistosomal antigens from infected mothers to their newborns. J Egypt Soc Parasitol 27 :773–780.
-
18
Rueff-Juy D, Faure M, Drapier A, Cazenave P, 1999. Role of maternal Ig in the induction of C kappa-specific CD8+ T cell tolerance. J Immunol 161 :721–728.
-
19
Ichino M, Mor G, Conover J, Weiss WR, Takeno M, Ishii KJ, Klinman DM, 1999. Factors associated with the development of neonatal tolerance after the administration of a plasmid DNA vaccine. J Immunol 162 :3814–3818.
-
20
Schwartz RH, 1989. Acquisition of immunologic self-tolerance. Cell 57 :1073–1081.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 209 | 126 | 5 |
| Full Text Views | 409 | 6 | 0 |
| PDF Downloads | 42 | 8 | 0 |