Identification of Isomorphic Malaria Vectors using a DNA Probe

Sakol Panyim Departments of Biochemistry and Biology, Faculty of Science, Mahidol University, Rama VI Road, Bangkok, Thailand

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Sukkid Yasothornsrikul Departments of Biochemistry and Biology, Faculty of Science, Mahidol University, Rama VI Road, Bangkok, Thailand

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Sumalee Tungpradubkul Departments of Biochemistry and Biology, Faculty of Science, Mahidol University, Rama VI Road, Bangkok, Thailand

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Visut Baimai Departments of Biochemistry and Biology, Faculty of Science, Mahidol University, Rama VI Road, Bangkok, Thailand

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Ronald Rosenberg Walter Reed Army Institute of Research, Washington, DC 20307

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R. G. Andre Walter Reed Army Institute of Research, Washington, DC 20307

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C. A. Green Departments of Biochemistry and Biology, Faculty of Science, Mahidol University, Rama VI Road, Bangkok, Thailand

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DOI:
https://doi.org/10.4269/ajtmh.1988.38.47
Volume/Issue:
Volume 38: Issue 1
Page(s):
47–49
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About 7,000 recombinant clones, derived from chromosomally-identified families of wild-caught females of Anopheles dirus species D, were screened. The most promising clone was totally specific to species D when tested against single F1 females of all four species of the complex. In fresh specimens the clone was positive for DNA levels 150 times less than the normal DNA content of single individuals. Fresh adult males and females, larvae, and dried specimens have been successfully identified. The clone was sequenced; it is 124 bp long and appears to be repeated in the genome about 1.8 × 104 times.

Author Notes

Copyright:
Copyright © 1988 by The American Society of Tropical Medicine and Hygiene 1988
Accepted:
24 Jul 1987
|
Published Online:
Jan 1988
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
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