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-
1.
Franzén L. , Westin G. , Shabo R. , Aslund L. , Perlmann H. , Persson T. , Wigzell H. , Pettersson U. , 1984. Analysis of clinical specimens by hybridization with probe containing repetitive DNA from Plasmodium falciparum. Lancet, 1: 525–528.
-
2.
Holmberg M. , Björkman A. , Franzén L. , Aslund L. , Lebbad M. , Pettersson U. , Wigzell H. , 1986. Diagnosis of Plasmodium falciparum infection by spot hybridization assay: Specificity, sensitivity and field applicability. Bull. W.H.O., 64: 579–585.
-
3.
McLaughlin G. L. , Edlind T. D. , Campbell G. H. , Eller R. F. , Ihler G. M. , 1985. Detection of Plasmodium falciparum using a synthetic DNA probe.. Am. J. Trap. Med. Hyg., 34: 837–840.
-
4.
Enea V. , 1986. Sensitive and specific DNA probe for detection of Plasmodium falciparum. Malec. Cell. Biol., 6: 321–324.
-
5.
Oquendo P. , Goman M. , Mackay M. , Langsley G. , Walliker D. , Scaife J. , 1986. Characterization of a repetitive DNA sequence from the malaria parasite, Plasmodium falciparum. Malec. Biochem. Parasitol., 18: 89–101.
-
6.
Barker R. Jr. , Suebsaeng L. , Rooney W. , Alecrim G. , Dourado H. , Wirth D. , 1986. Specific DNA probe for the diagnosis of Plasmodium falciparum malaria. Science, 231: 1434–1436.
-
7.
Guntaka R. , Rao A. , Green T. , Collins W. , 1986. Specific detection of Plasmodium falciparum malaria by a molecularly cloned DNA probe. Biochem. Biophys. Res. Comm., 138: 363–368.
-
8.
Snow R. W. , Rowan K. , Greenwood B. M. , 1987. A trial of permethrin treated bed nets in the prevention of malaria in Gambian children. Trans. R. Soc. Trap. Med. Hyg., 81: (In press).
-
9.
Åslund L. , Franzén L. , Westin G. , Persson T. , Wigzell H. , Pettersson U. , 1985. Highly reiterated non-coding sequence in the genome of Plasmodium falciparum is composed of 21 base-pair tandem repeats. J. Malec. Biol., 185: 509–516.
-
10.
Rigby P. , Dieckman M. , Rhodes C. , Berg P. , 1977. Labeling deoxyribonucleic acid to high specific activity in vitro by Nick translation with DNA polymerase. Intl. J. Malec. Biol., 113: 237–251.
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Use of a DNA Hybridization Assay for the Detection of Plasmodium Falciparumin Field Trials
Martin Holmberg
Martin Holmberg
* Department of Immunology, Karolinska Institute, Stockholm, Sweden
† Department of Medical Genetics, Uppsala University, Uppsala, Sweden
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Fiona C. Shenton
Fiona C. Shenton
‡ Medical Research Council Laboratories, Fajara, The Gambia
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Lena Franzén
Lena Franzén
* Department of Immunology, Karolinska Institute, Stockholm, Sweden
† Department of Medical Genetics, Uppsala University, Uppsala, Sweden
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Kebba Janneh
Kebba Janneh
‡ Medical Research Council Laboratories, Fajara, The Gambia
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Robert W. Snow
Robert W. Snow
‡ Medical Research Council Laboratories, Fajara, The Gambia
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Ulf Pettersson
Ulf Pettersson
† Department of Medical Genetics, Uppsala University, Uppsala, Sweden
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Hans Wigzell
Hans Wigzell
* Department of Immunology, Karolinska Institute, Stockholm, Sweden
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Brian M. Greenwood
Brian M. Greenwood
‡ Medical Research Council Laboratories, Fajara, The Gambia
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A DNA probe consisting of 21 base pair repeats obtained from a Tanzanian isolate of Plasmodium falciparum, cloned in pBR322 and labeled with 32P by nick translation was used to detect malaria parasitemia in samples obtained during a malaria survey undertaken in The Gambia. In an initial trial the hybridization assay had a specificity for P. falciparum of 100% and a sensitivity of 68%. False negative results were obtained only on samples with low parasitemia. Assay of red cells collected during an earlier malaria survey which had been stored for 1 year at ™20°C gave a higher level of sensitivity (85%), suggesting a beneficial effect from freezing and thawing. This was confirmed by examining in the same assay red cells processed immediately after collection and after 2 weeks of storage at ™20°C. Freezing and thawing gave a 21% increase in positivity, and a sensitivity of 100% was achieved with the frozen samples. Quantitation of autoradiographs by visual inspection and by scintillation counting gave a reasonable correlation with parasite counts. The DNA hybridization assay has considerable promise as an epidemiological tool.
Author Notes
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Reference Manager
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-
1.
Franzén L. , Westin G. , Shabo R. , Aslund L. , Perlmann H. , Persson T. , Wigzell H. , Pettersson U. , 1984. Analysis of clinical specimens by hybridization with probe containing repetitive DNA from Plasmodium falciparum. Lancet, 1: 525–528.
-
2.
Holmberg M. , Björkman A. , Franzén L. , Aslund L. , Lebbad M. , Pettersson U. , Wigzell H. , 1986. Diagnosis of Plasmodium falciparum infection by spot hybridization assay: Specificity, sensitivity and field applicability. Bull. W.H.O., 64: 579–585.
-
3.
McLaughlin G. L. , Edlind T. D. , Campbell G. H. , Eller R. F. , Ihler G. M. , 1985. Detection of Plasmodium falciparum using a synthetic DNA probe.. Am. J. Trap. Med. Hyg., 34: 837–840.
-
4.
Enea V. , 1986. Sensitive and specific DNA probe for detection of Plasmodium falciparum. Malec. Cell. Biol., 6: 321–324.
-
5.
Oquendo P. , Goman M. , Mackay M. , Langsley G. , Walliker D. , Scaife J. , 1986. Characterization of a repetitive DNA sequence from the malaria parasite, Plasmodium falciparum. Malec. Biochem. Parasitol., 18: 89–101.
-
6.
Barker R. Jr. , Suebsaeng L. , Rooney W. , Alecrim G. , Dourado H. , Wirth D. , 1986. Specific DNA probe for the diagnosis of Plasmodium falciparum malaria. Science, 231: 1434–1436.
-
7.
Guntaka R. , Rao A. , Green T. , Collins W. , 1986. Specific detection of Plasmodium falciparum malaria by a molecularly cloned DNA probe. Biochem. Biophys. Res. Comm., 138: 363–368.
-
8.
Snow R. W. , Rowan K. , Greenwood B. M. , 1987. A trial of permethrin treated bed nets in the prevention of malaria in Gambian children. Trans. R. Soc. Trap. Med. Hyg., 81: (In press).
-
9.
Åslund L. , Franzén L. , Westin G. , Persson T. , Wigzell H. , Pettersson U. , 1985. Highly reiterated non-coding sequence in the genome of Plasmodium falciparum is composed of 21 base-pair tandem repeats. J. Malec. Biol., 185: 509–516.
-
10.
Rigby P. , Dieckman M. , Rhodes C. , Berg P. , 1977. Labeling deoxyribonucleic acid to high specific activity in vitro by Nick translation with DNA polymerase. Intl. J. Malec. Biol., 113: 237–251.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 797 | 504 | 58 |
| Full Text Views | 12 | 7 | 0 |
| PDF Downloads | 5 | 3 | 0 |