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Immuno-Electron Microscopic Observations on Plasmodium Knowlesi Sporozoites: Localization of Protective Antigen and its Precursors
Edward Fine
Edward Fine
Institute of Pathology, Case Western Reserve University, Cleveland, Ohio 44106
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Masamichi Aikawa
Masamichi Aikawa
Institute of Pathology, Case Western Reserve University, Cleveland, Ohio 44106
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Alan H. Cochrane
Alan H. Cochrane
Division of Parasitology, Department of Microbiology, New York University Medical Center, New York, New York 10016
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Ruth S. Nussenzweig
Ruth S. Nussenzweig
Division of Parasitology, Department of Microbiology, New York University Medical Center, New York, New York 10016
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Cryoultramicrotomy, an antisporozoite monoclonal antibody (2G3) and protein A-gold particles were used to determine the distribution of the protective surface antigen (Pk 42) of sporozoites of Plasmodium knowlesi and its precursors (Pk 52 and Pk 50). Gold particles bound uniformly to both the outer plasma and the inner pellicular membranes, indicating a uniform distribution of antigen on these membranes. Intracellular gold particles were frequently found to be associated with micronemes, but less frequently with rhoptries. Limited proteolysis of P. knowlesi sporozoites totally eliminated binding of label to the outer plasma membrane. In contrast, distribution of label on the inner pellicular membranes, rhoptries, and micronemes of trypsinized sporozoites was relatively unaltered. On the basis of the present and earlier data obtained upon metabolic labeling of these parasites, it is likely that the antigen associated with the intracellular organelles corresponds to the precursor polypeptides Pk 52 and Pk 50.
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