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Isolation of Viruses from Female Culex Tritaeniorhynchus in Aedes Albopictus Cell Cultures
Akira Igarashi
Akira Igarashi
Department of Preventive Medicine, Research Institute for Microbial Diseases, Osaka University, Department of Public Health, Osaka Prefectural Institute of Public Health, Yamada-kami, Suita-city, Osaka, Japan
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Kazuo Buei
Kazuo Buei
Department of Preventive Medicine, Research Institute for Microbial Diseases, Osaka University, Department of Public Health, Osaka Prefectural Institute of Public Health, Yamada-kami, Suita-city, Osaka, Japan
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Noboru Ueba
Noboru Ueba
Department of Preventive Medicine, Research Institute for Microbial Diseases, Osaka University, Department of Public Health, Osaka Prefectural Institute of Public Health, Yamada-kami, Suita-city, Osaka, Japan
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Masahiro Yoshida
Masahiro Yoshida
Department of Preventive Medicine, Research Institute for Microbial Diseases, Osaka University, Department of Public Health, Osaka Prefectural Institute of Public Health, Yamada-kami, Suita-city, Osaka, Japan
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Sumiyo Ito
Sumiyo Ito
Department of Preventive Medicine, Research Institute for Microbial Diseases, Osaka University, Department of Public Health, Osaka Prefectural Institute of Public Health, Yamada-kami, Suita-city, Osaka, Japan
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Hiroshi Nakamura
Hiroshi Nakamura
Department of Preventive Medicine, Research Institute for Microbial Diseases, Osaka University, Department of Public Health, Osaka Prefectural Institute of Public Health, Yamada-kami, Suita-city, Osaka, Japan
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Fuyoko Sasao
Fuyoko Sasao
Department of Preventive Medicine, Research Institute for Microbial Diseases, Osaka University, Department of Public Health, Osaka Prefectural Institute of Public Health, Yamada-kami, Suita-city, Osaka, Japan
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Konosuke Fukai
Konosuke Fukai
Department of Preventive Medicine, Research Institute for Microbial Diseases, Osaka University, Department of Public Health, Osaka Prefectural Institute of Public Health, Yamada-kami, Suita-city, Osaka, Japan
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Isolation of viruses from female Culex tritaeniorhynchus was performed by inoculation of specimens into Aedes albopictus clone C6/36 cells as well as into suckling mouse brains (SMB). Altogether, 32,812 mosquitoes in 349 pools were processed from the specimens collected in Osaka Prefecture during the summer of 1978. Thirty-nine strains of Japanese encephalitis virus (JEV) were isolated in C6/36 cells in contrast to 27 strains in SMB. Twentyfour pools yielded JEV both in cell cultures and in SMB, 10 yielded JEV only in cell cultures, and three only in SMB. Two strains of Getah (GET) virus were isolated in cell cultures but not in SMB. Two of the 23 plaque isolates of a GET strain showed significantly lower titers of plaque formation on BHK21 cells or SMB-LD50, compared with the titer of plaque formation on C6/36 cells at 28°C. In addition, many unidentified filterable agents were detected by plaque formation on C6/36 cells.
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| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 464 | 211 | 11 |
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| PDF Downloads | 12 | 2 | 0 |