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A Simple Technique for the Detection of Dengue Antigen in Mosquitoes by Immunofluorescence
Timothy T. Kuberski
Timothy T. Kuberski
Pacific Research Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Honolulu, Hawaii 96806
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Leon Rosen
Leon Rosen
Pacific Research Section, Laboratory of Parasitic Diseases, National Institute of Allergy and Infectious Diseases, National Institutes of Health, Honolulu, Hawaii 96806
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Dengue antigen could be demonstrated by a direct fluorescent antibody technique in simple head squashes of either male or female Aedes albopictus mosquitoes infected with any of the four dengue serotypes. Dengue viruses can be isolated rapidly and simply by combining this technique with that of parenteral inoculation of mosquitoes with test material.
Author Notes
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 747 | 301 | 73 |
| Full Text Views | 51 | 8 | 3 |
| PDF Downloads | 68 | 14 | 4 |