ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Millar SB , Cox-Singh J , 2015. Human infections with Plasmodium knowlesi-zoonotic malaria. Clin Microbiol Infect 21: 640–648.
-
2.
World Health Organization , 2022. World Malaria Report 2022. Geneva, Switzerland: WHO.
-
3.
Moody A , 2002. Rapid diagnostic tests for malaria parasites. Clin Microbiol Rev 5: 66–78.
-
4.
Kilian AHD , Metzger WG , Mutschelknauss EJ , Kabagambe G , Langi P , Korte R , von Sonnenburg F , 2000. Reliability of malaria microscopy in epidemiological studies: results of quality control. Trop Med Int Health 5: 3–8.
-
5.
Zimmerman PA , Howes RE , 2015. Malaria diagnosis for malaria elimination. Curr Opin Infect Dis 28: 446–454.
-
6.
Ocker R , Prompunjai Y , Chutipongvivate S , Karanis P , 2016. Malaria diagnosis by loop-mediated isothermal amplification (LAMP) in Thailand. Rev Inst Med Trop São Paulo 58: 27.
-
7.
Lau YL , Lai MY , Fong MY , Jelip J , Mahmud R , 2016. Loop-mediated isothermal amplification assay for identification of five human Plasmodium species in Malaysia. Am J Trop Med Hyg 94: 336–339.
-
8.
Lucchi NW et al., 2016. Evaluation of the illumigene malaria LAMP: a robust molecular diagnostic tool for malaria parasites. Sci Rep 6: 36808.
-
9.
Hopkins H et al., 2013. Highly sensitive detection of malaria parasitemia in a malaria-endemic setting: performance of a new loop-mediated isothermal amplification kit in a remote clinic in Uganda .J Infect Dis 208: 645–652.
-
10.
Vallejo AF , Martínez NL , González IJ , Arévalo-Herrera M , Herrera S , 2015. Evaluation of the loop mediated isothermal DNA amplification (LAMP) kit for malaria diagnosis in P. vivax endemic settings of Colombia. PLoS Negl Trop Dis 9: e3453.
-
11.
Oriero EC , Okebe J , Jacobs J , Van JP , Nwakanma D , Alessandro UD , 2015. Diagnostic performance of a novel loop-mediated isothermal amplification (LAMP) assay targeting the apicoplast genome for malaria diagnosis in a field setting in sub-Saharan Africa. Malar J 14: 396.
-
12.
Mohon AN , Elahi R , Khan WA , Haque R , Sullivan DJ Jr. , Alam MS , 2014. A new visually improved and sensitive loop mediated isothermal amplification (LAMP) for diagnosis of symptomatic falciparum malaria. Acta Trop 134: 52–57.
-
13.
Britton S et al., 2016. Sensitive detection of Plasmodium vivax using a high-throughput, colourimetric loop mediated isothermal amplification (HtLAMP) platform: a potential novel tool for malaria elimination .PLoS Negl Trop Dis 10: e0004443.
-
14.
Goto M , Honda E , Ogura A , Nomoto A , Hanaki KIJB , 2009. Colorimetric detection of loop-mediated isothermal amplification reaction by using hydroxy naphthol blue. Biotechniques 46: 167–172.
-
15.
Tomita N , Mori Y , Kanda H , Notomi T , 2008. Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products. Nat Protoc 3: 877–882.
-
16.
Parida M , Horioke K , Ishida H , Dash PK , Saxena P , Jana AM , Islam MA , Inoue S , Hosaka N , Morita K , 2005. Rapid detection and differentiation of dengue virus serotypes by a real-time reverse transcription-loop-mediated isothermal amplification assay. J Clin Microbiol 43: 2895–2903.
-
17.
Tanner NA , Zhang Y , Evans TCJB , 2015. Visual detection of isothermal nucleic acid amplification using pH-sensitive dyes. Biotechniques 58: 59.
-
18.
Jaroenram W , Cecere P , Pompa PP , 2019. Xylenol orange-based loop-mediated DNA isothermal amplification for sensitive naked-eye detection of Escherichia coli. J Microbiol Methods 156: 9–14.
-
19.
Yang X et al., 2021. Rapid and visual differentiation of Mycobacterium tuberculosis from the Mycobacterium tuberculosis complex using multiplex loop-mediated isothermal amplification coupled with a nanoparticle-based lateral flow biosensor. Front Microbiol 12: 708658.
-
20.
Tavakoli-Koopaei R , Javadi-Zarnaghi F , Aboutalebian S , Mirhendi H , 2023. Malachite green-based detection of SARS-CoV-2 by one-step reverse transcription loop-mediated isothermal amplification. Iran J Sci 47: 359–367.
-
21.
Nzelu CO , Gomez EA , Caceres AG , Sakurai T , Martini-Robles L , Uezato H , Mimori T , Katakura K , Hashiguchi Y , Kato H , 2014. Development of a loop-mediated isothermal amplification method for rapid mass-screening of sand flies for Leishmania infection. Acta Trop 132: 1–6.
-
22.
Jothikumar P , Narayanan J , Hill VR , 2014. Visual end point detection of Escherichia coli O157:H7 using isothermal genome exponential amplification reaction (GEAR) assay and malachite green. J Microbiol Methods 98: 122–127.
-
23.
Imwong M , Tanomsing N , Pukrittayakamee S , Day NPJ , White NJ , Snounou G , 2009. Spurious amplification of a Plasmodium vivax small-subunit RNA gene by use of primers currently used to detect P. knowlesi. J Clin Microbiol 47: 4173–4175.
-
24.
Snounou G , Viriyakosol S , Zhu XP , Jarra W , Pieiro L , do Rosario VE , Thaithong S , Brown KN , 1993. High sensitivity of detection of human malaria parasites by the use of nested polymerase chain reaction. Mol Biochem Parasitol 61: 315–320.
-
25.
Gachugia J , Chebore W , Otieno K , Ngugi CW , Godana A , Kariuki S , 2020. Evaluation of the colorimetric malachite green loop-mediated isothermal amplification (MG-LAMP) assay for the detection of malaria species at two different health facilities in a malaria endemic area of western Kenya. Malar J 19: 329.
-
26.
Barazorda KA et al., 2021. Validation study of boil & spin malachite green loop mediated isothermal amplification (B&S MG LAMP) versus microscopy for malaria detection in the Peruvian Amazon. PLoS One 16: e0258722.
-
27.
Park GS , Ku KB , Baek SH , Kim SJ , Kim SJ , Kim BT , Maeng JS , 2020. Development of reverse transcription loop-mediated isothermal amplification assays targeting severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2). J Mol Diagn 22: 729–735.
-
28.
Lucchi NW , Ljolje D , Silva-Flannery L , Udhayakumar V , 2016. Use of malachite green-loop-mediated isothermal amplification for detection of Plasmodium spp. parasites. PLoS One 11: e0151437.
-
29.
Zou Y , Mason MG , Botella JR , 2020. Evaluation and improvement of isothermal amplification methods for point-of-need plant disease diagnostics. PLoS One 15: e0235216.
-
30.
Wang DG , Brewster JD , Paul M , Tomasula PM , 2015. Two methods for increased specificity and sensitivity in loop-mediated isothermal amplification. Molecules 20: 6048–6059.
-
31.
Lee PYZ , Lai MY , Lau YL , 2020. Elimination of carryover contamination in loop-mediated isothermal assay for detection of human malaria. Trop Biomed 37: 1124–1128.
-
32.
Lucchi NW , Demas A , Narayanan J , Sumari D , Kabanywanyi A , Kachur SP , Barnwell JW , Udhayakumar V , 2010. Real-time fluorescence loop-mediated isothermal amplification for the diagnosis of malaria. PLoS One 5: e13733.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 1321 | 1321 | 79 |
| Full Text Views | 74 | 74 | 0 |
| PDF Downloads | 71 | 71 | 0 |
Recombinase-Aided Loop-Mediated Isothermal Amplification on Human Plasmodium knowlesi
Meng Yee Lai
Meng Yee Lai
Department of Parasitology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia;
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Mohd Hafizi Abdul Hamid
Mohd Hafizi Abdul Hamid
Vector Borne Disease Sector, Ministry of Health, Putrajaya, Malaysia
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Jenarun Jelip
Jenarun Jelip
Vector Borne Disease Sector, Ministry of Health, Putrajaya, Malaysia
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Rose Nani Mudin
Rose Nani Mudin
Vector Borne Disease Sector, Ministry of Health, Putrajaya, Malaysia
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Yee Ling Lau
Yee Ling Lau
Department of Parasitology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia;
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ABSTRACT.
Loop-mediated isothermal amplification (LAMP) is a nucleic acid amplification technique that can amplify specific nucleic acids at a constant temperature (63–65°C) within a short period (<1 hour). In this study, we report the utilization of recombinase-aided LAMP to specifically amplify the 18S sRNA of Plasmodium knowlesi. The method was built on a conventional LAMP assay by inclusion of an extra enzyme, namely recombinase, into the master mixture. With the addition of recombinase into the LAMP assay, the assay speed was executed within a time frame of less than 28 minutes at 65°C. We screened 55 P. knowlesi samples and 47 non–P. knowlesi samples. No cross-reactivity was observed for non–P. knowlesi samples, and the detection limit for recombinase-aided LAMP was one copy for P. knowlesi after LAMP amplification. It has been reported elsewhere that LAMP can be detected through fluorescent readout systems. Although such systems result in considerable limits of detection, the need for sophisticated equipment limits their use. Hence, we used here a colorimetric detection platform for the evaluation of the LAMP assay’s performance. This malachite green–based recombinase-aided LAMP assay enabled visualization of results with the naked eye. Negative samples were observed by a change in color from green to colorless, whereas positive samples remained green. Our results demonstrate that the LAMP assay developed here is a convenient, sensitive, and useful diagnostic tool for the rapid detection of knowlesi malaria parasites. This method is suitable for implementation in remote healthcare settings, where centralized laboratory facilities, funds, and clinicians are in short supply.
- Supplemental Materials (PDF 87.852 KB)
Author Notes
Financial support: This study was supported by Long Term Research Grant Scheme LRGS/1/2018/UM/01/1/4 (
Authors’ addresses: Meng Yee Lai and Yee Ling Lau, Department of Parasitology, Faculty of Medicine, Universiti Malaya, Kuala Lumpur, Malaysia, E-mails: mengylai11@um.edu.my and lauyeeling@um.edu.my. Mohd Hafizi Abdul Hamid, Jenarun Jelip, and Rose Nani Mudin, Vector Borne Disease Sector, Ministry of Health, Putrajaya, Malaysia, E-mails: dr.mhafizi@moh.gov.my, jenarun@moh.gov.my, and drrose@moh.gov.my.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Millar SB , Cox-Singh J , 2015. Human infections with Plasmodium knowlesi-zoonotic malaria. Clin Microbiol Infect 21: 640–648.
-
2.
World Health Organization , 2022. World Malaria Report 2022. Geneva, Switzerland: WHO.
-
3.
Moody A , 2002. Rapid diagnostic tests for malaria parasites. Clin Microbiol Rev 5: 66–78.
-
4.
Kilian AHD , Metzger WG , Mutschelknauss EJ , Kabagambe G , Langi P , Korte R , von Sonnenburg F , 2000. Reliability of malaria microscopy in epidemiological studies: results of quality control. Trop Med Int Health 5: 3–8.
-
5.
Zimmerman PA , Howes RE , 2015. Malaria diagnosis for malaria elimination. Curr Opin Infect Dis 28: 446–454.
-
6.
Ocker R , Prompunjai Y , Chutipongvivate S , Karanis P , 2016. Malaria diagnosis by loop-mediated isothermal amplification (LAMP) in Thailand. Rev Inst Med Trop São Paulo 58: 27.
-
7.
Lau YL , Lai MY , Fong MY , Jelip J , Mahmud R , 2016. Loop-mediated isothermal amplification assay for identification of five human Plasmodium species in Malaysia. Am J Trop Med Hyg 94: 336–339.
-
8.
Lucchi NW et al., 2016. Evaluation of the illumigene malaria LAMP: a robust molecular diagnostic tool for malaria parasites. Sci Rep 6: 36808.
-
9.
Hopkins H et al., 2013. Highly sensitive detection of malaria parasitemia in a malaria-endemic setting: performance of a new loop-mediated isothermal amplification kit in a remote clinic in Uganda .J Infect Dis 208: 645–652.
-
10.
Vallejo AF , Martínez NL , González IJ , Arévalo-Herrera M , Herrera S , 2015. Evaluation of the loop mediated isothermal DNA amplification (LAMP) kit for malaria diagnosis in P. vivax endemic settings of Colombia. PLoS Negl Trop Dis 9: e3453.
-
11.
Oriero EC , Okebe J , Jacobs J , Van JP , Nwakanma D , Alessandro UD , 2015. Diagnostic performance of a novel loop-mediated isothermal amplification (LAMP) assay targeting the apicoplast genome for malaria diagnosis in a field setting in sub-Saharan Africa. Malar J 14: 396.
-
12.
Mohon AN , Elahi R , Khan WA , Haque R , Sullivan DJ Jr. , Alam MS , 2014. A new visually improved and sensitive loop mediated isothermal amplification (LAMP) for diagnosis of symptomatic falciparum malaria. Acta Trop 134: 52–57.
-
13.
Britton S et al., 2016. Sensitive detection of Plasmodium vivax using a high-throughput, colourimetric loop mediated isothermal amplification (HtLAMP) platform: a potential novel tool for malaria elimination .PLoS Negl Trop Dis 10: e0004443.
-
14.
Goto M , Honda E , Ogura A , Nomoto A , Hanaki KIJB , 2009. Colorimetric detection of loop-mediated isothermal amplification reaction by using hydroxy naphthol blue. Biotechniques 46: 167–172.
-
15.
Tomita N , Mori Y , Kanda H , Notomi T , 2008. Loop-mediated isothermal amplification (LAMP) of gene sequences and simple visual detection of products. Nat Protoc 3: 877–882.
-
16.
Parida M , Horioke K , Ishida H , Dash PK , Saxena P , Jana AM , Islam MA , Inoue S , Hosaka N , Morita K , 2005. Rapid detection and differentiation of dengue virus serotypes by a real-time reverse transcription-loop-mediated isothermal amplification assay. J Clin Microbiol 43: 2895–2903.
-
17.
Tanner NA , Zhang Y , Evans TCJB , 2015. Visual detection of isothermal nucleic acid amplification using pH-sensitive dyes. Biotechniques 58: 59.
-
18.
Jaroenram W , Cecere P , Pompa PP , 2019. Xylenol orange-based loop-mediated DNA isothermal amplification for sensitive naked-eye detection of Escherichia coli. J Microbiol Methods 156: 9–14.
-
19.
Yang X et al., 2021. Rapid and visual differentiation of Mycobacterium tuberculosis from the Mycobacterium tuberculosis complex using multiplex loop-mediated isothermal amplification coupled with a nanoparticle-based lateral flow biosensor. Front Microbiol 12: 708658.
-
20.
Tavakoli-Koopaei R , Javadi-Zarnaghi F , Aboutalebian S , Mirhendi H , 2023. Malachite green-based detection of SARS-CoV-2 by one-step reverse transcription loop-mediated isothermal amplification. Iran J Sci 47: 359–367.
-
21.
Nzelu CO , Gomez EA , Caceres AG , Sakurai T , Martini-Robles L , Uezato H , Mimori T , Katakura K , Hashiguchi Y , Kato H , 2014. Development of a loop-mediated isothermal amplification method for rapid mass-screening of sand flies for Leishmania infection. Acta Trop 132: 1–6.
-
22.
Jothikumar P , Narayanan J , Hill VR , 2014. Visual end point detection of Escherichia coli O157:H7 using isothermal genome exponential amplification reaction (GEAR) assay and malachite green. J Microbiol Methods 98: 122–127.
-
23.
Imwong M , Tanomsing N , Pukrittayakamee S , Day NPJ , White NJ , Snounou G , 2009. Spurious amplification of a Plasmodium vivax small-subunit RNA gene by use of primers currently used to detect P. knowlesi. J Clin Microbiol 47: 4173–4175.
-
24.
Snounou G , Viriyakosol S , Zhu XP , Jarra W , Pieiro L , do Rosario VE , Thaithong S , Brown KN , 1993. High sensitivity of detection of human malaria parasites by the use of nested polymerase chain reaction. Mol Biochem Parasitol 61: 315–320.
-
25.
Gachugia J , Chebore W , Otieno K , Ngugi CW , Godana A , Kariuki S , 2020. Evaluation of the colorimetric malachite green loop-mediated isothermal amplification (MG-LAMP) assay for the detection of malaria species at two different health facilities in a malaria endemic area of western Kenya. Malar J 19: 329.
-
26.
Barazorda KA et al., 2021. Validation study of boil & spin malachite green loop mediated isothermal amplification (B&S MG LAMP) versus microscopy for malaria detection in the Peruvian Amazon. PLoS One 16: e0258722.
-
27.
Park GS , Ku KB , Baek SH , Kim SJ , Kim SJ , Kim BT , Maeng JS , 2020. Development of reverse transcription loop-mediated isothermal amplification assays targeting severe acute respiratory syndrome Coronavirus 2 (SARS-CoV-2). J Mol Diagn 22: 729–735.
-
28.
Lucchi NW , Ljolje D , Silva-Flannery L , Udhayakumar V , 2016. Use of malachite green-loop-mediated isothermal amplification for detection of Plasmodium spp. parasites. PLoS One 11: e0151437.
-
29.
Zou Y , Mason MG , Botella JR , 2020. Evaluation and improvement of isothermal amplification methods for point-of-need plant disease diagnostics. PLoS One 15: e0235216.
-
30.
Wang DG , Brewster JD , Paul M , Tomasula PM , 2015. Two methods for increased specificity and sensitivity in loop-mediated isothermal amplification. Molecules 20: 6048–6059.
-
31.
Lee PYZ , Lai MY , Lau YL , 2020. Elimination of carryover contamination in loop-mediated isothermal assay for detection of human malaria. Trop Biomed 37: 1124–1128.
-
32.
Lucchi NW , Demas A , Narayanan J , Sumari D , Kabanywanyi A , Kachur SP , Barnwell JW , Udhayakumar V , 2010. Real-time fluorescence loop-mediated isothermal amplification for the diagnosis of malaria. PLoS One 5: e13733.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 1321 | 1321 | 79 |
| Full Text Views | 74 | 74 | 0 |
| PDF Downloads | 71 | 71 | 0 |