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Yu XJ et al., 2011. Fever with thrombocytopenia associated with a novel bunyavirus in China. N Engl J Med 364: 1523–1532.
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Kuhn JH et al., 2020. 2020 taxonomic update for phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales. Arch Virol 165: 3023–3072.
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3.
Kim YR, Yun Y, Bae SG, Park D, Kim S, Lee JM, Cho NH, Kim YS, Lee KH, 2018. Severe fever with thrombocytopenia syndrome virus infection, South Korea, 2010. Emerg Infect Dis 24: 2103–2105.
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Sun JM et al., 2016. Factors associated with Severe Fever with Thrombocytopenia Syndrome infection and fatal outcome. Sci Rep 6: 33175.
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Kim UJ, Kim DM, Kim SE, Kang SJ, Jang HC, Park KH, Jung SI, 2018. Case report: detection of the identical virus in a patient presenting with severe fever with thrombocytopenia syndrome encephalopathy and the tick that bit her. BMC Infect Dis 18: 181.
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Yun SM, Lee WG, Ryou J, Yang SC, Park SW, Roh JY, Lee YJ, Park C, Han MG, 2014. Severe fever with thrombocytopenia syndrome virus in ticks collected from humans, South Korea, 2013. Emerg Infect Dis 20: 1358–1361.
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Hwang J, Kang JG, Oh SS, Chae JB, Cho YK, Cho YS, Lee H, Chae JS, 2017. Molecular detection of severe fever with thrombocytopenia syndrome virus (SFTSV) in feral cats from Seoul, Korea. Ticks Tick Borne Dis 8: 9–12.
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Clinical Usefulness of Nested Reverse-Transcription Polymerase Chain Reaction for the Diagnosis of Severe Fever with Thrombocytopenia Syndrome
Choon-Mee Kim
Choon-Mee Kim
Premedical Science, College of Medicine, Chosun University, Gwangju, Republic of Korea;
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Dong-Min Kim
Dong-Min Kim
Department of Internal Medicine, College of Medicine, Chosun University, Gwangju, Republic of Korea
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Na-Ra Yun
Na-Ra Yun
Department of Internal Medicine, College of Medicine, Chosun University, Gwangju, Republic of Korea
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ABSTRACT.
Severe fever with thrombocytopenia syndrome (SFTS), caused by SFTS virus (SFTSV) is an emerging tick-borne infectious disease. Few studies have assessed the clinical usefulness of nested reverse-transcription polymerase chain reaction (RT-PCR) for diagnosing SFTS. We performed conventional RT-PCR targeting the M segment, nested RT-PCR targeting M and S segments, and real-time RT-PCR targeting the S segment of SFTSV for four patients with suspected SFTS. Although conventional RT-PCR results for the first two patients were negative at admission, nested RT-PCR using the S or M targets was positive for the same samples. Likewise, in the other two patients, initial samples were confirmed positive in all three tests, but follow-up testing demonstrated negative conventional RT-PCR and positive nested RT-PCR results. Thus, delayed testing using conventional RT-PCR or real-time RT-PCR in symptomatic patients with SFTS may result in missed diagnoses, and compared with these methods, nested RT-PCR may increase the window for obtaining positive SFTSV PCR results. Meanwhile, the indirect immunofluorescence assay showed seroconversion to SFTSV antibodies in all four patients. Nested RT-PCR for SFTSV M and S segments could help diagnose SFTS in patients testing negative by conventional RT-PCR.
Author Notes
Disclosure: This study was approved by the Ethics in Human Research Committee of Chosun University Hospital under IRB No.2017-10-012. Data and materials are available upon request to the corresponding author.
Authors’ addresses: Choon-Mee Kim, Premedical Science, College of Medicine, Chosun University, Gwangju, Republic of Korea, E-mail: choonmee@chosun.ac.kr. Dong-Min Kim and Na-Ra Yun, Department of Internal Medicine, College of Medicine, Chosun University, Gwangju, Republic of Korea, E-mails: drongkim@chosun.ac.kr and shine@chosun.ac.kr.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Yu XJ et al., 2011. Fever with thrombocytopenia associated with a novel bunyavirus in China. N Engl J Med 364: 1523–1532.
-
2.
Kuhn JH et al., 2020. 2020 taxonomic update for phylum Negarnaviricota (Riboviria: Orthornavirae), including the large orders Bunyavirales and Mononegavirales. Arch Virol 165: 3023–3072.
-
3.
Kim YR, Yun Y, Bae SG, Park D, Kim S, Lee JM, Cho NH, Kim YS, Lee KH, 2018. Severe fever with thrombocytopenia syndrome virus infection, South Korea, 2010. Emerg Infect Dis 24: 2103–2105.
-
4.
Sun JM et al., 2016. Factors associated with Severe Fever with Thrombocytopenia Syndrome infection and fatal outcome. Sci Rep 6: 33175.
-
5.
Kim UJ, Kim DM, Kim SE, Kang SJ, Jang HC, Park KH, Jung SI, 2018. Case report: detection of the identical virus in a patient presenting with severe fever with thrombocytopenia syndrome encephalopathy and the tick that bit her. BMC Infect Dis 18: 181.
-
6.
Yun SM, Lee WG, Ryou J, Yang SC, Park SW, Roh JY, Lee YJ, Park C, Han MG, 2014. Severe fever with thrombocytopenia syndrome virus in ticks collected from humans, South Korea, 2013. Emerg Infect Dis 20: 1358–1361.
-
7.
Hwang J, Kang JG, Oh SS, Chae JB, Cho YK, Cho YS, Lee H, Chae JS, 2017. Molecular detection of severe fever with thrombocytopenia syndrome virus (SFTSV) in feral cats from Seoul, Korea. Ticks Tick Borne Dis 8: 9–12.
-
8.
Zhang YZ et al., 2012. Hemorrhagic fever caused by a novel bunyavirus in China: pathogenesis and correlates of fatal outcome. Clin Infect Dis 54: 527–533.
-
9.
Han MA, Kim CM, Kim DM, Yun NR, Park SW, Han MG, Lee WJ, 2018. Seroprevalence of severe fever with thrombocytopenia syndrome virus antibodies in rural areas, South Korea. Emerg Infect Dis 24: 872–874.
-
10.
Park SW, Han MG, Yun SM, Park C, Lee WJ, Ryou J, 2014. Severe fever with thrombocytopenia syndrome virus, South Korea, 2013. Emerg Infect Dis 20: 1880–1882.
-
11.
Choi SJ, et al., 2016. Severe fever with thrombocytopenia syndrome in South Korea, 2013–2015. PLoS Negl Trop Dis 10: e0005264.
-
12.
Kato H, Yamagishi T, Shimada T, Matsui T, Shimojima M, Saijo M, Oishi K, Japa SERG, 2016. Epidemiological and clinical features of severe fever with thrombocytopenia syndrome in Japan, 2013–2014. PLoS One 11: e0165207.
-
13.
Yoshikawa T et al., 2014. Sensitive and specific PCR systems for detection of both Chinese and Japanese severe fever with thrombocytopenia syndrome virus strains and prediction of patient survival based on viral load. J Clin Microbiol 52: 3325–3333.
-
14.
Hu B, Cai K, Liu M, Li WJ, Xu JQ, Qiu F, Zhan JB, 2018. Laboratory detection and molecular phylogenetic analysis of severe fever with thrombocytopenia syndrome virus in Hubei Province, central China. Arch Virol 163: 3243–3254.
-
15.
Li Z, Bao C, Hu J, Gao C, Zhang N, Xiang H, Cardona CJ, Xing Z, 2019. Susceptibility of spotted doves (Streptopelia chinensis) to experimental infection with the severe fever with thrombocytopenia syndrome phlebovirus. PLoS Negl Trop Dis 13: e0006982.
-
16.
Kim DM, Yun NR, Yang TY, Lee JH, Yang JT, Shim SK, Choi EN, Park MY, Lee SH, 2006. Usefulness of nested PCR for the diagnosis of scrub typhus in clinical practice: a prospective study. Am J Trop Med Hyg 75: 542–545.
-
17.
Dupin N, Buffet M, Marcelin AG, Lamotte C, Gorin I, Ait-Arkoub Z, Treluyer JM, Bui P, Calvez V, Peytavin G, 2002. HIV and antiretroviral drug distribution in plasma and fat tissue of HIV-infected patients with lipodystrophy. AIDS 16: 2419–2424.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 1780 | 816 | 83 |
| Full Text Views | 230 | 41 | 1 |
| PDF Downloads | 235 | 23 | 1 |