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1
Vaughn DW, Hoke CH Jr, Yoksan S, LaChance R, Innis BL, Rice RM, Bhamarapravati N, 1996. Testing of a dengue 2 live-attenuated vaccine (strain 16681 PDK 53) in ten American volunteers. Vaccine 14 :329–336.
-
2
Edelman R, Tacket CO, Wasserman SS, Vaughn DW, Eckels KH, Dubois DR, Summers PL, Hoke CH, 1994. A live attenuated dengue-1 vaccine candidate (45AZ5) passaged in primary dog kidney cell culture is attenuated and immunogenic for humans. J Infect Dis 170 :1448–1455.
-
3
Hoke CH Jr, Malinoski FJ, Eckels KH, Scott RM, Dubois DR, Summers PL, Simms T, Burrous J, Hasty SE, Bancroft WH, 1990. Preparation of an attenuated dengue 4 (341750 Carib) virus vaccine. II. Safety and immunogenicity in humans. Am J Trop Med Hyg 43 :219–226.
-
4
Eckels KH, Scott RM, Bancroft WH, Brown J, Dubois DR, Summers PL, Russell PK, Halstead SB, 1984. Selection of attenuated dengue 4 viruses by serial passage in primary kidney cells. V. Human response to immunization with a candidate vaccine prepared in fetal rhesus lung cells. Am J Trop Med Hyg 33 :684–689.
-
5
Mosmann TR, 1986. Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins. J Immunol 136 :2348–2357.
-
6
Peters M, 1996. Actions of cytokines on the immune response and viral interactions: an overview. Hepatology 23 :909–916.
-
7
Conrad DH, Ben-Sasson SZ, Le Gross G, Finkleman FD, Paul WE, 1990. Infection with Nipostrongylus braziliensis.J Exp Med 171 :1497–1508.
-
8
Pacsa AS, Agarwal R, Elbishbishi EA, Chaturvedi UC, Nagar R, Mustafa AS, 2000. Role of interleukin-12 in patients with dengue hemorrhagic fever. FEMS Immunol Med Microbiol 28 :151–155.
-
9
Kurane I, Innis BL, Nimmannitya S, Nisalak A, Meager A, Janus J, Ennis FA, 1991. Activation of T lymphocytes in dengue virus infections. High levels of soluble interleukin 2 receptor, soluble CD4, soluble CD8, interleukin 2, and interferon-gamma in sera of children with dengue. J Clin Invest 88 :1473–1480.
-
10
Johnson AJ, Roehrig JT, 1999. New mouse model for dengue virus vaccine testing. J Virol 73 :783–786.
-
11
Kontny U, Kurane I, Ennis FA, 1988. Gamma interferon augments Fc gamma receptor-mediated dengue virus infection of human monocytic cells. J Virol 62 :3928–3933.
-
12
Sittisombut N, Maneekarn N, Kanjanahaluethai A, Kasinrerk W, Viputtikul K, Supawadee J, 1995. Lack of augmenting effect of interferon-gamma on dengue virus multiplication in human peripheral blood monocytes. J Med Virol 45 :43–49.
-
13
Kurane I, Brinton MA, Samson AL, Ennis FA, 1991. Dengue virus-specific, human CD4+ CD8- cytotoxic T-cell clones: multiple patterns of virus cross-reactivity recognized by NS3-specific T-cell clones. J Virol 65 :1823–1828.
-
14
Kurane I, Rothman AL, Livingston PG, Green S, Gagnon SJ, Janus J, Innis BL, Nimmannitya S, Nisalak A, Ennis FA, 1994. Immunopathologic mechanisms of dengue hemor-rhagic fever and dengue shock syndrome. Arch Virol (suppl) 9 :59–64.
-
15
Dharakul T, Kurane I, Bhamarapravati N, Yoksan S, Vaughn DW, Hoke CH, Ennis FA, 1994. Dengue virus-specific memory T cell responses in human volunteers receiving a live attenuated dengue virus type 2 candidate vaccine. J Infect Dis 170 :27–33.
-
16
Kurane I, Meager A, Ennis FA, 1989. Dengue virus-specific human T cell clones. Serotype crossreactive proliferation, interferon gamma production, and cytotoxic activity. J Exp Med 170 :763–775.
-
17
Kurane I, Innis BL, Hoke CH Jr, Eckels KH, Meager A, Janus J, Ennis FA, 1995. T cell activation in vivo by dengue virus infection. J Clin Lab Immunol 46 :35–40.
| Past two years | Past Year | Past 30 Days | |
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| Abstract Views | 868 | 816 | 52 |
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SEROTYPE-SPECIFIC TH1 RESPONSES IN RECIPIENTS OF TWO DOSES OF CANDIDATE LIVE-ATTENUATED DENGUE VIRUS VACCINES
WILLIAM GWINN
WILLIAM GWINN
Department of Virus Diseases, Walter Reed Army Institute of Research, Silver Spring, Maryland
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WELLINGTON SUN
WELLINGTON SUN
Department of Virus Diseases, Walter Reed Army Institute of Research, Silver Spring, Maryland
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BRUCE L. INNIS
BRUCE L. INNIS
Department of Virus Diseases, Walter Reed Army Institute of Research, Silver Spring, Maryland
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JEFFREY CAUDILL
JEFFREY CAUDILL
Department of Virus Diseases, Walter Reed Army Institute of Research, Silver Spring, Maryland
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ALAN D. KING
ALAN D. KING
Department of Virus Diseases, Walter Reed Army Institute of Research, Silver Spring, Maryland
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As part of a larger vaccine study, peripheral blood mononuclear cells (PBMC) were collected from volunteers for analysis of vaccine- induced T cell responses. The PBMC were re-stimulated in vitro with live dengue virus and assayed for Th1 or Th2 memory cell responses. Re-stimulated PBMC from the volunteers predominantly secreted interferon-γ. Little interleukin-4 (IL-4) or IL-10 secretion was detected, indicating a Th1 type of T cell response. The interferon-γ response was primarily serotype-specific with some serotype cross-reactivity. T cell depletion studies showed that the interferon-γ was being secreted by CD4+ T lymphocytes and/or by cells other than CD8+ T lymphocytes that were being stimulated by the CD4+ T lymphocytes. CD3+ or CD8+ T cell depletion showed that granzyme B mRNA expression correlated with the presence of CD4+ T lymphocytes. However, depletion of CD4+ T cells after four days of stimulation indicated that the granzyme B mRNA was produced by cells in culture other than lymphocytes. In summary, an antigen-specific Th1 type T cell response was seen as a response to vaccination using live attenuated dengue virus.
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RefWorks
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-
1
Vaughn DW, Hoke CH Jr, Yoksan S, LaChance R, Innis BL, Rice RM, Bhamarapravati N, 1996. Testing of a dengue 2 live-attenuated vaccine (strain 16681 PDK 53) in ten American volunteers. Vaccine 14 :329–336.
-
2
Edelman R, Tacket CO, Wasserman SS, Vaughn DW, Eckels KH, Dubois DR, Summers PL, Hoke CH, 1994. A live attenuated dengue-1 vaccine candidate (45AZ5) passaged in primary dog kidney cell culture is attenuated and immunogenic for humans. J Infect Dis 170 :1448–1455.
-
3
Hoke CH Jr, Malinoski FJ, Eckels KH, Scott RM, Dubois DR, Summers PL, Simms T, Burrous J, Hasty SE, Bancroft WH, 1990. Preparation of an attenuated dengue 4 (341750 Carib) virus vaccine. II. Safety and immunogenicity in humans. Am J Trop Med Hyg 43 :219–226.
-
4
Eckels KH, Scott RM, Bancroft WH, Brown J, Dubois DR, Summers PL, Russell PK, Halstead SB, 1984. Selection of attenuated dengue 4 viruses by serial passage in primary kidney cells. V. Human response to immunization with a candidate vaccine prepared in fetal rhesus lung cells. Am J Trop Med Hyg 33 :684–689.
-
5
Mosmann TR, 1986. Two types of murine helper T cell clone. I. Definition according to profiles of lymphokine activities and secreted proteins. J Immunol 136 :2348–2357.
-
6
Peters M, 1996. Actions of cytokines on the immune response and viral interactions: an overview. Hepatology 23 :909–916.
-
7
Conrad DH, Ben-Sasson SZ, Le Gross G, Finkleman FD, Paul WE, 1990. Infection with Nipostrongylus braziliensis.J Exp Med 171 :1497–1508.
-
8
Pacsa AS, Agarwal R, Elbishbishi EA, Chaturvedi UC, Nagar R, Mustafa AS, 2000. Role of interleukin-12 in patients with dengue hemorrhagic fever. FEMS Immunol Med Microbiol 28 :151–155.
-
9
Kurane I, Innis BL, Nimmannitya S, Nisalak A, Meager A, Janus J, Ennis FA, 1991. Activation of T lymphocytes in dengue virus infections. High levels of soluble interleukin 2 receptor, soluble CD4, soluble CD8, interleukin 2, and interferon-gamma in sera of children with dengue. J Clin Invest 88 :1473–1480.
-
10
Johnson AJ, Roehrig JT, 1999. New mouse model for dengue virus vaccine testing. J Virol 73 :783–786.
-
11
Kontny U, Kurane I, Ennis FA, 1988. Gamma interferon augments Fc gamma receptor-mediated dengue virus infection of human monocytic cells. J Virol 62 :3928–3933.
-
12
Sittisombut N, Maneekarn N, Kanjanahaluethai A, Kasinrerk W, Viputtikul K, Supawadee J, 1995. Lack of augmenting effect of interferon-gamma on dengue virus multiplication in human peripheral blood monocytes. J Med Virol 45 :43–49.
-
13
Kurane I, Brinton MA, Samson AL, Ennis FA, 1991. Dengue virus-specific, human CD4+ CD8- cytotoxic T-cell clones: multiple patterns of virus cross-reactivity recognized by NS3-specific T-cell clones. J Virol 65 :1823–1828.
-
14
Kurane I, Rothman AL, Livingston PG, Green S, Gagnon SJ, Janus J, Innis BL, Nimmannitya S, Nisalak A, Ennis FA, 1994. Immunopathologic mechanisms of dengue hemor-rhagic fever and dengue shock syndrome. Arch Virol (suppl) 9 :59–64.
-
15
Dharakul T, Kurane I, Bhamarapravati N, Yoksan S, Vaughn DW, Hoke CH, Ennis FA, 1994. Dengue virus-specific memory T cell responses in human volunteers receiving a live attenuated dengue virus type 2 candidate vaccine. J Infect Dis 170 :27–33.
-
16
Kurane I, Meager A, Ennis FA, 1989. Dengue virus-specific human T cell clones. Serotype crossreactive proliferation, interferon gamma production, and cytotoxic activity. J Exp Med 170 :763–775.
-
17
Kurane I, Innis BL, Hoke CH Jr, Eckels KH, Meager A, Janus J, Ennis FA, 1995. T cell activation in vivo by dengue virus infection. J Clin Lab Immunol 46 :35–40.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 868 | 816 | 52 |
| Full Text Views | 484 | 15 | 2 |
| PDF Downloads | 102 | 18 | 2 |