ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Pediatric Dengue Vaccine Initiative, 2010. Global Burden of Dengue: Pediatric Dengue Vaccine Initiative. Available at: http://www.pdvi.org/about_dengue/GBD.asp.
-
2.
Kyle JL, Harris E, 2008. Global spread and persistence of dengue. Annu Rev Microbiol 62: 71–92.
-
3.
Nimmannitya S, 1997. Dengue hemorrhagic fever: diagnosis and management. Gubler DJ, Kuno G, eds. Dengue and Dengue Hemorrhagic Fever. Oxford, UK: CAB International, 133–145.
-
4.
Tricou V, Minh NN, Van TP, Lee SJ, Farrar J, Wills B, Tran HT, Simmons CP, 2010. A randomized controlled trial of chloroquine for the treatment of dengue in Vietnamese adults. PLoS Negl Trop Dis 4: e785.
-
5.
World Health Organization, 1997. Dengue Hemorrhagic Fever: Diagnosis, Treatment, Prevention and Control. Geneva, Switzerland: World Health Organization.
-
6.
Balmaseda A, Hammond SN, Perez MA, Cuadra R, Solano S, Rocha J, Idiaquez W, Harris E, 2005. Short report: assessment of the World Health Organization scheme for classification of dengue severity in Nicaragua. Am J Trop Med Hyg 73: 1059–1062.
-
7.
Deen JL, Harris E, Wills B, Balmaseda A, Hammond SN, Rocha C, Dung NM, Hung NT, Hien TT, Farrar JJ, 2006. The WHO dengue classification and case definitions: time for a reassessment. Lancet 368: 170–173.
-
8.
Bandyopadhyay S, Lum LC, Kroeger A, 2006. Classifying dengue: a review of the difficulties in using the WHO case classification for dengue haemorrhagic fever. Trop Med Int Health 11: 1238–1255.
-
9.
World Health Organization, 2009. Dengue: Guidelines for Diagnosis, Treatment Prevention and Control. Geneva, Switzerland: World Health Organization.
-
10.
Potts JA, Rothman AL, 2008. Clinical and laboratory features that distinguish dengue from other febrile illnesses in endemic populations. Trop Med Int Health 13: 1328–1340.
-
11.
Young PR, Hilditch PA, Bletchly C, Halloran W, 2000. An antigen capture enzyme-linked immunosorbent assay reveals high levels of the dengue virus protein NS1 in the sera of infected patients. J Clin Microbiol 38: 1053–1057.
-
12.
Alcon S, Talarmin A, Debruyne M, Falconar A, Deubel V, Flamand M, 2002. Enzyme-linked immunosorbent assay specific to dengue virus type 1 nonstructural protein NS1 reveals circulation of the antigen in the blood during the acute phase of disease in patients experiencing primary or secondary infections. J Clin Microbiol 40: 376–381.
-
13.
Mackenzie JM, Jones MK, Young PR, 1996. Immunolocalization of the dengue virus nonstructural glycoprotein NS1 suggests a role in viral RNA replication. Virology 220: 232–240.
-
14.
Low JG, Ooi EE, Tolfvenstam T, Leo YS, Hibberd ML, Ng LC, Lai YL, Yap GS, Li CS, Vasudevan SG, Ong A, 2006. Early dengue infection and outcome study (EDEN)—study design and preliminary findings. Ann Acad Med Singapore 35: 783–789.
-
15.
Lai YL, Chung YK, Tan HC, Yap HF, Yap G, Ooi EE, Ng LC, 2007. Cost-effective real-time reverse transcriptase PCR (RT-PCR) to screen for dengue virus followed by rapid single-tube multiplex RT-PCR for serotyping of the virus. J Clin Microbiol 45: 935–941.
-
16.
Henchal EA, Gentry MK, McCown JM, Brandt WE, 1982. Dengue virus-specific and flavivirus group determinants identified with monoclonal antibodies by indirect immunofluorescence. Am J Trop Med Hyg 31: 830–836.
-
17.
Henchal EA, McCown JM, Seguin MC, Gentry MK, Brandt WE, 1983. Rapid identification of dengue virus isolates by using monoclonal antibodies in an indirect immunofluorescence assay. Am J Trop Med Hyg 32: 164–169.
-
18.
Ooi EE, Goh KT, Gubler DJ, 2006. Dengue prevention and 35 years of vector control in Singapore. Emerg Infect Dis 12: 887–893.
-
19.
Ting SH, Tan HC, Wong WK, Ng ML, Chan SH, Ooi EE, 2004. Seroepidemiology of neutralizing antibodies to Japanese encephalitis virus in Singapore: continued transmission despite abolishment of pig farming? Acta Trop 92: 187–191.
-
20.
Tricou V, Vu HT, Quynh NV, Nguyen CV, Tran HT, Farrar J, Wills B, Simmons CP, 2010. Comparison of two dengue NS1 rapid tests for sensitivity, specificity and relationship to viraemia and antibody responses. BMC Infect Dis 10: 142.
-
21.
Hang VT, Nguyet NM, Trung DT, Tricou V, Yoksan S, Dung NM, Van Ngoc T, Hien TT, Farrar J, Wills B, Simmons CP, 2009. Diagnostic accuracy of NS1 ELISA and lateral flow rapid tests for dengue sensitivity, specificity and relationship to viraemia and antibody responses. PLoS Negl Trop Dis 3: e360.
-
22.
Kalayanarooj S, Vaughn DW, Nimmannitya S, Green S, Suntayakorn S, Kunentrasai N, Viramitrachai W, Ratanachu-eke S, Kiatpolpoj S, Innis BL, Rothman AL, Nisalak A, Ennis FA, 1997. Early clinical and laboratory indicators of acute dengue illness. J Infect Dis 176: 313–321.
-
23.
Binh PT, Matheus S, Huong VT, Deparis X, Marechal V, 2009. Early clinical and biological features of severe clinical manifestations of dengue in Vietnamese adults. J Clin Virol 45: 276–280.
-
24.
Ramos MM, Tomashek KM, Arguello DF, Luxemburger C, Quinones L, Lang J, Munoz-Jordan JL, 2009. Early clinical features of dengue infection in Puerto Rico. Trans R Soc Trop Med Hyg 103: 878–884.
-
25.
Ramirez AH, Moros Z, Comach G, Zambrano J, Bravo L, Pinto B, Vielma S, Cardier J, Liprandi F, 2009. Evaluation of dengue NS1 antigen detection tests with acute sera from patients infected with dengue virus in Venezuela. Diagn Microbiol Infect Dis 65: 247–253.
-
26.
Chaiyaratana W, Chuansumrit A, Pongthanapisith V, Tangnararatchakit K, Lertwongrath S, Yoksan S, 2009. Evaluation of dengue nonstructural protein 1 antigen strip for the rapid diagnosis of patients with dengue infection. Diagn Microbiol Infect Dis 64: 83–84.
-
27.
Dussart P, Petit L, Labeau B, Bremand L, Leduc A, Moua D, Matheus S, Baril L, 2008. Evaluation of two new commercial tests for the diagnosis of acute dengue virus infection using NS1 antigen detection in human serum. PLoS Negl Trop Dis 2: e280.
-
28.
Kittigul L, Pitakarnjanakul P, Sujirarat D, Siripanichgon K, 2007. The differences of clinical manifestations and laboratory findings in children and adults with dengue virus infection. J Clin Virol 39: 76–81.
-
29.
Guzman MG, Jaenisch T, Gaczkowski R, Ty Hang VT, Sekaran SD, Kroeger A, Vazquez S, Ruiz D, Martinez E, Mercado JC, Balmaseda A, Harris E, Dimano E, Leano PS, Yoksan S, Villegas E, Benduzu H, Villalobos I, Farrar J, Simmons CP, 2010. Multi-country evaluation of the sensitivity and specificity of two commercially-available NS1 ELISA assays for dengue diagnosis. PLoS Negl Trop Dis 4: e811.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 551 | 442 | 40 |
| Full Text Views | 612 | 19 | 1 |
| PDF Downloads | 331 | 20 | 1 |
Evaluation of the NS1 Rapid Test and the WHO Dengue Classification Schemes for Use as Bedside Diagnosis of Acute Dengue Fever in Adults
Shera Chaterji
Shera Chaterji
Program in Emerging Infectious Diseases, Duke—National University of Singapore (NUS) Graduate Medical School, Singapore; Centre for Quantitative Medicine, Duke—NUS Graduate Medical School, Singapore; Communicable Diseases Centre, Tan Tock Seng Hospital, Singapore
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John Carson Allen Jr.
John Carson Allen Jr.
Program in Emerging Infectious Diseases, Duke—National University of Singapore (NUS) Graduate Medical School, Singapore; Centre for Quantitative Medicine, Duke—NUS Graduate Medical School, Singapore; Communicable Diseases Centre, Tan Tock Seng Hospital, Singapore
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Angelia Chow
Angelia Chow
Program in Emerging Infectious Diseases, Duke—National University of Singapore (NUS) Graduate Medical School, Singapore; Centre for Quantitative Medicine, Duke—NUS Graduate Medical School, Singapore; Communicable Diseases Centre, Tan Tock Seng Hospital, Singapore
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Yee-Sin Leo
Yee-Sin Leo
Program in Emerging Infectious Diseases, Duke—National University of Singapore (NUS) Graduate Medical School, Singapore; Centre for Quantitative Medicine, Duke—NUS Graduate Medical School, Singapore; Communicable Diseases Centre, Tan Tock Seng Hospital, Singapore
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Eng-Eong Ooi
Eng-Eong Ooi
Program in Emerging Infectious Diseases, Duke—National University of Singapore (NUS) Graduate Medical School, Singapore; Centre for Quantitative Medicine, Duke—NUS Graduate Medical School, Singapore; Communicable Diseases Centre, Tan Tock Seng Hospital, Singapore
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Because healthcare facilities in many dengue endemic countries lack laboratory support, early dengue diagnosis must rely on either clinical recognition or a bedside diagnostic test. We evaluated the sensitivity and specificity of the 1997 and 2009 World Health Organization (WHO) dengue classification schemes and the NS1 strip test in acute sera from 154 virologically confirmed dengue patients and 200 patients with other febrile illnesses. Both WHO classification schemes had high sensitivity but lacked specificity. The NS1 strip test had high specificity, but its sensitivity was significantly lower in secondary compared with primary dengue infections. Differences in viral serotypes did not affect the performance of any of the three diagnostic approaches. Taken collectively, our findings indicate that the 1997 WHO dengue case definition can be used to exclude dengue, and the NS1 strip test can be used to confirm dengue infection, although the latter should be interpreted with caution in regions where secondary dengue infection is prevalent.
Author Notes
Financial support: This study was funded by the National Medical Research Council, Singapore.
Authors' addresses: Shera Chaterji, Angelia Chow, and Eng-Eong Ooi, Program in Emerging Infectious Diseases, Duke—NUS Graduate Medical School, Singapore, E-mails: schaterji@gmail.com, angelia.chow@duke-nus.edu.sg, and engeong.ooi@duke-nus.edu.sg. John Carson Allen Jr., Centre for Quantitative Medicine, Duke–NUS Graduate Medical School, Singapore, E-mail: john.allen@duke-nus.edu.sg. Yee-Sin Leo, Communicable Diseases Centre, Tan Tock Seng Hospital, Singapore, E-mail: yee_sin_leo@ttsh.com.sg.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Pediatric Dengue Vaccine Initiative, 2010. Global Burden of Dengue: Pediatric Dengue Vaccine Initiative. Available at: http://www.pdvi.org/about_dengue/GBD.asp.
-
2.
Kyle JL, Harris E, 2008. Global spread and persistence of dengue. Annu Rev Microbiol 62: 71–92.
-
3.
Nimmannitya S, 1997. Dengue hemorrhagic fever: diagnosis and management. Gubler DJ, Kuno G, eds. Dengue and Dengue Hemorrhagic Fever. Oxford, UK: CAB International, 133–145.
-
4.
Tricou V, Minh NN, Van TP, Lee SJ, Farrar J, Wills B, Tran HT, Simmons CP, 2010. A randomized controlled trial of chloroquine for the treatment of dengue in Vietnamese adults. PLoS Negl Trop Dis 4: e785.
-
5.
World Health Organization, 1997. Dengue Hemorrhagic Fever: Diagnosis, Treatment, Prevention and Control. Geneva, Switzerland: World Health Organization.
-
6.
Balmaseda A, Hammond SN, Perez MA, Cuadra R, Solano S, Rocha J, Idiaquez W, Harris E, 2005. Short report: assessment of the World Health Organization scheme for classification of dengue severity in Nicaragua. Am J Trop Med Hyg 73: 1059–1062.
-
7.
Deen JL, Harris E, Wills B, Balmaseda A, Hammond SN, Rocha C, Dung NM, Hung NT, Hien TT, Farrar JJ, 2006. The WHO dengue classification and case definitions: time for a reassessment. Lancet 368: 170–173.
-
8.
Bandyopadhyay S, Lum LC, Kroeger A, 2006. Classifying dengue: a review of the difficulties in using the WHO case classification for dengue haemorrhagic fever. Trop Med Int Health 11: 1238–1255.
-
9.
World Health Organization, 2009. Dengue: Guidelines for Diagnosis, Treatment Prevention and Control. Geneva, Switzerland: World Health Organization.
-
10.
Potts JA, Rothman AL, 2008. Clinical and laboratory features that distinguish dengue from other febrile illnesses in endemic populations. Trop Med Int Health 13: 1328–1340.
-
11.
Young PR, Hilditch PA, Bletchly C, Halloran W, 2000. An antigen capture enzyme-linked immunosorbent assay reveals high levels of the dengue virus protein NS1 in the sera of infected patients. J Clin Microbiol 38: 1053–1057.
-
12.
Alcon S, Talarmin A, Debruyne M, Falconar A, Deubel V, Flamand M, 2002. Enzyme-linked immunosorbent assay specific to dengue virus type 1 nonstructural protein NS1 reveals circulation of the antigen in the blood during the acute phase of disease in patients experiencing primary or secondary infections. J Clin Microbiol 40: 376–381.
-
13.
Mackenzie JM, Jones MK, Young PR, 1996. Immunolocalization of the dengue virus nonstructural glycoprotein NS1 suggests a role in viral RNA replication. Virology 220: 232–240.
-
14.
Low JG, Ooi EE, Tolfvenstam T, Leo YS, Hibberd ML, Ng LC, Lai YL, Yap GS, Li CS, Vasudevan SG, Ong A, 2006. Early dengue infection and outcome study (EDEN)—study design and preliminary findings. Ann Acad Med Singapore 35: 783–789.
-
15.
Lai YL, Chung YK, Tan HC, Yap HF, Yap G, Ooi EE, Ng LC, 2007. Cost-effective real-time reverse transcriptase PCR (RT-PCR) to screen for dengue virus followed by rapid single-tube multiplex RT-PCR for serotyping of the virus. J Clin Microbiol 45: 935–941.
-
16.
Henchal EA, Gentry MK, McCown JM, Brandt WE, 1982. Dengue virus-specific and flavivirus group determinants identified with monoclonal antibodies by indirect immunofluorescence. Am J Trop Med Hyg 31: 830–836.
-
17.
Henchal EA, McCown JM, Seguin MC, Gentry MK, Brandt WE, 1983. Rapid identification of dengue virus isolates by using monoclonal antibodies in an indirect immunofluorescence assay. Am J Trop Med Hyg 32: 164–169.
-
18.
Ooi EE, Goh KT, Gubler DJ, 2006. Dengue prevention and 35 years of vector control in Singapore. Emerg Infect Dis 12: 887–893.
-
19.
Ting SH, Tan HC, Wong WK, Ng ML, Chan SH, Ooi EE, 2004. Seroepidemiology of neutralizing antibodies to Japanese encephalitis virus in Singapore: continued transmission despite abolishment of pig farming? Acta Trop 92: 187–191.
-
20.
Tricou V, Vu HT, Quynh NV, Nguyen CV, Tran HT, Farrar J, Wills B, Simmons CP, 2010. Comparison of two dengue NS1 rapid tests for sensitivity, specificity and relationship to viraemia and antibody responses. BMC Infect Dis 10: 142.
-
21.
Hang VT, Nguyet NM, Trung DT, Tricou V, Yoksan S, Dung NM, Van Ngoc T, Hien TT, Farrar J, Wills B, Simmons CP, 2009. Diagnostic accuracy of NS1 ELISA and lateral flow rapid tests for dengue sensitivity, specificity and relationship to viraemia and antibody responses. PLoS Negl Trop Dis 3: e360.
-
22.
Kalayanarooj S, Vaughn DW, Nimmannitya S, Green S, Suntayakorn S, Kunentrasai N, Viramitrachai W, Ratanachu-eke S, Kiatpolpoj S, Innis BL, Rothman AL, Nisalak A, Ennis FA, 1997. Early clinical and laboratory indicators of acute dengue illness. J Infect Dis 176: 313–321.
-
23.
Binh PT, Matheus S, Huong VT, Deparis X, Marechal V, 2009. Early clinical and biological features of severe clinical manifestations of dengue in Vietnamese adults. J Clin Virol 45: 276–280.
-
24.
Ramos MM, Tomashek KM, Arguello DF, Luxemburger C, Quinones L, Lang J, Munoz-Jordan JL, 2009. Early clinical features of dengue infection in Puerto Rico. Trans R Soc Trop Med Hyg 103: 878–884.
-
25.
Ramirez AH, Moros Z, Comach G, Zambrano J, Bravo L, Pinto B, Vielma S, Cardier J, Liprandi F, 2009. Evaluation of dengue NS1 antigen detection tests with acute sera from patients infected with dengue virus in Venezuela. Diagn Microbiol Infect Dis 65: 247–253.
-
26.
Chaiyaratana W, Chuansumrit A, Pongthanapisith V, Tangnararatchakit K, Lertwongrath S, Yoksan S, 2009. Evaluation of dengue nonstructural protein 1 antigen strip for the rapid diagnosis of patients with dengue infection. Diagn Microbiol Infect Dis 64: 83–84.
-
27.
Dussart P, Petit L, Labeau B, Bremand L, Leduc A, Moua D, Matheus S, Baril L, 2008. Evaluation of two new commercial tests for the diagnosis of acute dengue virus infection using NS1 antigen detection in human serum. PLoS Negl Trop Dis 2: e280.
-
28.
Kittigul L, Pitakarnjanakul P, Sujirarat D, Siripanichgon K, 2007. The differences of clinical manifestations and laboratory findings in children and adults with dengue virus infection. J Clin Virol 39: 76–81.
-
29.
Guzman MG, Jaenisch T, Gaczkowski R, Ty Hang VT, Sekaran SD, Kroeger A, Vazquez S, Ruiz D, Martinez E, Mercado JC, Balmaseda A, Harris E, Dimano E, Leano PS, Yoksan S, Villegas E, Benduzu H, Villalobos I, Farrar J, Simmons CP, 2010. Multi-country evaluation of the sensitivity and specificity of two commercially-available NS1 ELISA assays for dengue diagnosis. PLoS Negl Trop Dis 4: e811.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 551 | 442 | 40 |
| Full Text Views | 612 | 19 | 1 |
| PDF Downloads | 331 | 20 | 1 |