Isolation of the Causative Agent of Hantavirus Pulmonary Syndrome

L. H. Elliott Special Pathogens Branch, Molecular Pathology and Ultrastructure Activities, Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, and Division of Vector-Borne Infectious Diseases, National Center for Infectious Disease, Centers for Disease Control and Prevention, Atlanta, Georgia and Fort Collins, Colorado

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T. G. Ksiazek Special Pathogens Branch, Molecular Pathology and Ultrastructure Activities, Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, and Division of Vector-Borne Infectious Diseases, National Center for Infectious Disease, Centers for Disease Control and Prevention, Atlanta, Georgia and Fort Collins, Colorado

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P. E. Rollin Special Pathogens Branch, Molecular Pathology and Ultrastructure Activities, Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, and Division of Vector-Borne Infectious Diseases, National Center for Infectious Disease, Centers for Disease Control and Prevention, Atlanta, Georgia and Fort Collins, Colorado

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C. F. Spiropoulou Special Pathogens Branch, Molecular Pathology and Ultrastructure Activities, Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, and Division of Vector-Borne Infectious Diseases, National Center for Infectious Disease, Centers for Disease Control and Prevention, Atlanta, Georgia and Fort Collins, Colorado

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S. Morzunov Special Pathogens Branch, Molecular Pathology and Ultrastructure Activities, Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, and Division of Vector-Borne Infectious Diseases, National Center for Infectious Disease, Centers for Disease Control and Prevention, Atlanta, Georgia and Fort Collins, Colorado

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M. Monroe Special Pathogens Branch, Molecular Pathology and Ultrastructure Activities, Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, and Division of Vector-Borne Infectious Diseases, National Center for Infectious Disease, Centers for Disease Control and Prevention, Atlanta, Georgia and Fort Collins, Colorado

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C. S. Goldsmith Special Pathogens Branch, Molecular Pathology and Ultrastructure Activities, Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, and Division of Vector-Borne Infectious Diseases, National Center for Infectious Disease, Centers for Disease Control and Prevention, Atlanta, Georgia and Fort Collins, Colorado

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C. D. Humphrey Special Pathogens Branch, Molecular Pathology and Ultrastructure Activities, Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, and Division of Vector-Borne Infectious Diseases, National Center for Infectious Disease, Centers for Disease Control and Prevention, Atlanta, Georgia and Fort Collins, Colorado

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S. R. Zaki Special Pathogens Branch, Molecular Pathology and Ultrastructure Activities, Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, and Division of Vector-Borne Infectious Diseases, National Center for Infectious Disease, Centers for Disease Control and Prevention, Atlanta, Georgia and Fort Collins, Colorado

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J. W. Krebs Special Pathogens Branch, Molecular Pathology and Ultrastructure Activities, Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, and Division of Vector-Borne Infectious Diseases, National Center for Infectious Disease, Centers for Disease Control and Prevention, Atlanta, Georgia and Fort Collins, Colorado

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G. Maupin Special Pathogens Branch, Molecular Pathology and Ultrastructure Activities, Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, and Division of Vector-Borne Infectious Diseases, National Center for Infectious Disease, Centers for Disease Control and Prevention, Atlanta, Georgia and Fort Collins, Colorado

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K. Gage Special Pathogens Branch, Molecular Pathology and Ultrastructure Activities, Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, and Division of Vector-Borne Infectious Diseases, National Center for Infectious Disease, Centers for Disease Control and Prevention, Atlanta, Georgia and Fort Collins, Colorado

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J. E. Childs Special Pathogens Branch, Molecular Pathology and Ultrastructure Activities, Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, and Division of Vector-Borne Infectious Diseases, National Center for Infectious Disease, Centers for Disease Control and Prevention, Atlanta, Georgia and Fort Collins, Colorado

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S. T. Nichol Special Pathogens Branch, Molecular Pathology and Ultrastructure Activities, Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, and Division of Vector-Borne Infectious Diseases, National Center for Infectious Disease, Centers for Disease Control and Prevention, Atlanta, Georgia and Fort Collins, Colorado

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C. J. Peters Special Pathogens Branch, Molecular Pathology and Ultrastructure Activities, Viral and Rickettsial Zoonoses Branch, Division of Viral and Rickettsial Diseases, and Division of Vector-Borne Infectious Diseases, National Center for Infectious Disease, Centers for Disease Control and Prevention, Atlanta, Georgia and Fort Collins, Colorado

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DOI:
https://doi.org/10.4269/ajtmh.1994.51.102
Volume/Issue:
Volume 51: Issue 1
Page(s):
102–108
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Investigation of a recent outbreak of acute respiratory illness in the southwestern United States resulted in the recognition of a new disease, hantavirus pulmonary syndrome (HPS) with high mortality. Different animals and cell lines were used in attempts to isolate the causative agent. A previously unknown hantavirus was passaged in laboratory-bred deer mice, recovered from lung tissues of a deer mouse, Peromyscus maniculatus, and propagated in the E6 clone of Vero cells. Virus antigen was readily detected in the infected cells by an indirect immunofluorescence assay, using convalescent-phase sera from HPS patients. By electron microscopy, the virus was shown to have the typical morphologic features of members of the genus Hantavirus, family Bunyaviridae. Virus sequences corresponded to those previously detected by a nested reverse transcriptase-polymerase chain reaction assay of hantavirus-infected specimens from rodents and humans. This newly recognized virus, the etiologic agent of HPS, has been tentatively named Muerto Canyon virus.

Author Notes

Copyright:
Copyright © 1994 by The American Society of Tropical Medicine and Hygiene 1994
Published Online:
Jul 1994
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
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