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Mark-Release-Recapture of Packed and Shipped Aedes aegypti with Wolbachia: Implications for Conducting Remote Incompatible Insect Technique Programs
Johanna R. Ohm
joohm@verily.com
Johanna R. Ohm
Verily Life Sciences LLC, South San Francisco, California;
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Amy Lynd
Amy Lynd
Green VI, Virgin Gorda, British Virgin Islands
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Austin McGowan
Austin McGowan
Verily Life Sciences LLC, South San Francisco, California;
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Angel Cupid
Angel Cupid
Green VI, Virgin Gorda, British Virgin Islands
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Vernessa Bellot
Vernessa Bellot
Green VI, Virgin Gorda, British Virgin Islands
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James Q. Le
James Q. Le
Verily Life Sciences LLC, South San Francisco, California;
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Evdoxia Kakani
Evdoxia Kakani
Verily Life Sciences LLC, South San Francisco, California;
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Josh Livni
Josh Livni
Verily Life Sciences LLC, South San Francisco, California;
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Jacob E. Crawford
Jacob E. Crawford
Verily Life Sciences LLC, South San Francisco, California;
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Bradley J. White
Bradley J. White
Verily Life Sciences LLC, South San Francisco, California;
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Male mosquitoes containing the endosymbiont Wolbachia (Wb+) can be used as a tool to suppress wild mosquito populations through a technique termed incompatible insect technique (IIT). IIT programs reduce wild mosquitoes via incompatible matings between released males and wild females to reduce the number of viable offspring produced in the next generation. Successful programs rely on regular release of incompatible males to outcompete wild males for female mates. Past IIT programs have relied on local production of Wb+ males to support regular releases of incompatible males. Here, we evaluated the survival and dispersal of packed and shipped Wb+ Aedes aegypti males in mark-release-recapture studies at a release site in the British Virgin Islands (BVI), separated by over 3,600 miles from the centralized production facility. Released mosquitoes were recaptured using BG-Sentinel 2 traps collected daily for up to 7 days after release. Wb+ male mosquitoes packed and shipped from a centralized production facility performed similarly to males that were locally reared in the BVI in survival, dispersal, and recapture rates. Our results support the conclusion that packing and shipping live Wb+ male mosquitoes does not impact their ability to survive and disperse in release sites and suggests that IIT mosquito control programs can feasibly be conducted nearly anywhere in the world without the need for local mosquito production facilities.
Author Notes
Disclosures: J. R. Ohm, J. E. Crawford, B. J. White, J. Q. Le, J. Livni, E. Kakani, and A. McGowan are employees of Verily Life Sciences, which operates the Debug program, a for-profit program working toward reducing the global burden of mosquito-borne disease.
Authors’ contributions: J. R. Ohm, J. E. Crawford, and B. J. White conceptualized design; A. Lynd led field methods and community outreach, supported by V. Bellot; J. R. Ohm, A. Lynd, A. Cupid, B. J. White, V. Bellot, and A. McGowan collected samples and sorted mosquitoes; J. Q. Le and J. R. Ohm performed lab validation and fluorescent marker; E. Kakani performed mating competitive assays; J. Q. Le, J. R. Ohm, and A. McGowan performed molecular analysis; J. Livni provided methods and software for data collection; J. R. Ohm performed data analysis; and J. R. Ohm wrote manuscript.
Current contact information: Johanna R. Ohm, Austin McGowan, James Q. Le, Evdoxia Kakani, Josh Livni, Jacob E. Crawford, and Bradley J. White, Google LLC, South San Francisco, CA, E-mails: joohm@google.com, mcgowanau@google.com, jamesqle@google.com, ekakani@google.com, jlivni@google.com, jacobcrawford@google.com, and bradwhite@google.com. Amy Lynd, Angel Cupid, and Vernessa Bellot, Green VI, Virgin Gorda, British Virgin Islands, E-mails: amy@greenvi.org, angel@greenvi.org, vernessa@greenvi.org.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 0 | 0 | 0 |
| Full Text Views | 3599 | 3599 | 707 |
| PDF Downloads | 285 | 285 | 61 |