- Introduction
- Methods
- Statistical analysis.
- Ethical aspects.
- Ethical aspects of animal experiments.
- Methods of laboratory diagnosis.
- Parasitological methods.
- Laboratory maintenance of the S. mansoni experimental cycle.
- Periovular reaction.
- Detection of IgM antibodies against antigens of the S. mansoni digestive tract.
- Antibody detection of S. mansoni egg antigens.
- Results
- Discussion
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
World Health Organization, 2001. Prevention and Control of Schistosomiasis and the Soil-Transmitted Helminthiasis. Report of a WHO Expert Committee. Geneva: WHO, 73.
-
2.
Chitsulo L, Engels D, Montresor A, Savioli L, 2000. The global status of schistosomiasis and its control. Acta Trop 77: 41–51.
-
3.
World Health Organization, 2001. Report of Informal Consultation on Schistosomiasis in Low Transmission Areas: Control Strategies and Criteria for Elimination. London, 10–13 April 2000. Geneva: WHO, 52.
-
4.
Alarcón de Noya BA, Guevara RR, Colmenares C, Losada S, Noya O, 2006. Low transmission areas of schistosomiasis in Venezuela: consequences on the diagnosis, treatment, and control. Mem Inst Oswaldo Cruz 101: 29–35.
-
5.
Ministério da Saúde, 2012. Secretaria de Vigilância a Saúde. Sistema de Informação do Programa de Controle da Esquistossomose. Sistema de Informação de Agravos em Saúde/2012. Casos confirmados de Esquistossomose, Brasil, Grandes Regiões e Unidades Federadas, 1995 a 2011. Brasil. (Dados preliminares, sujeitos à revisão).
-
6.
São Paulo, 2007. Secretaria de Estado da Saúde. Centro de Vigilância Epidemiológica. Coordenadoria de Controle de Doenças. Vigilância Epidemiológica e Controle da Esquistossomose-Normas e Instruções. São Paulo, 45.
-
7.
Katz N, Chaves A, Pellegrino J, 1972. A simple device for quantitative stool thick smear technique in schistosomiasis mansoni. Rev Inst Med Trop Sao Paulo 14: 397–400.
-
8.
Kongs A, Marks G, Verle P, Van der Stuyft P, 2001. The unreliability of the Kato-Katz technique limits its usefulness for evaluating S. mansoni infections. Trop Med Int Health 6: 163–169.
-
9.
Engels D, Sinzinkayo E, Gryseels B, 1996. Day-to-day egg count fluctuation in Schistosoma mansoni infection and its operational implications. Am J Trop Med Hyg 54: 319–324.
-
10.
Teesdale CH, Fahringer K, Chistulo L, 1985. Egg count variation and sensitivity of a thin smear technique for the diagnosis of Schistosoma mansoni. Trans R Soc Trop Med Hyg 79: 369–373.
-
11.
World Health Organization, 2012. Schistosomiasis. Fact Sheet No. 115. Geneva: WHO.
-
12.
Teles HM, de Carvalho ME, Santos Ferreira C, Zacharias F, de Lima VR, Fadel ML, 2002. Schistosomiasis mansoni in Bananal (State of São Paulo, Brazil): I. Efficiency of diagnostic and treatment procedures. Mem Inst Oswaldo Cruz 97: 181–186.
-
13.
Alarcón de Noya BA, Noya O, Balzan C, Cesari IM, 1992. New approaches for the control and eradication of schistosomiasis in Venezuela. Mem Inst Oswaldo Cruz 87: 227–231.
-
14.
Wang W, Li Y, Li H, Xing Y, Qu G, Dai J, Liang Y, 2012. Immunodiagnostic efficacy of detection of Schistosoma japonicum human infections in China: a meta analysis. Asian Pac J Trop Med 5: 15–23.
-
15.
Kanamura HY, Dias LC, da Silva RM, Glasser CM, Patucci RM, Vellosa SA, Antunes JL, 1998. A comparative epidemiologic study of specific antibodies (IgM and IgA) and parasitological findings in an endemic area of low transmission of Schistosoma mansoni. Rev Inst Med Trop Sao Paulo 40: 85–91.
-
16.
Burlandy-Soares LC, de Souza Dias LC, Kanamura HY, de Oliveira EJ, Ciaravolo RM, 2003. Schistosomiasis mansoni: follow-up of control program based on parasitologic and serologic methods in a Brazilian community of low endemicity. Mem Inst Oswaldo Cruz 98: 853–859.
-
17.
Oliver-Gonzalez J, 1954. Anti-egg precipitins in the serum of humans infected with Schistosoma mansoni. J Infect Dis 95: 86–91.
-
18.
Spencer L, Alarcón de Noya B, Noya O, Masroua G, 1991. Comparative analysis between the circumoval precipitin test and ELISA with raw antigens for the diagnosis of schistosomiasis in Venezuela. G E N 45: 77–83 Spanish.
-
19.
Hillyer GV, Tiben ER, Knight WB, Gómez de Rios I, Pelley RP, 1979. Immunodiagnosis of infection with Schistosoma mansoni: comparison of ELISA, radioimmunoassay, and precipitation tests performed with antigens from eggs. Am J Trop Med Hyg 28: 661–669.
-
20.
Rio de Janeiro, 2011. Pactuação das Ações de Vigilância em Saúde. Pactuação das ações prioritárias da PAVS para o biênio 2010–2011. Monitorar situação epidemiológica da esquistossomose nos municípios das áreas endêmicas do Rio de Janeiro: Porciúncula, Paracambi, Sumidouro, Barra Mansa, Duas Barras e Rio de Janeiro.
-
21.
Rio de Janeiro, 2008. Secretaria Municipal de Saúde, Coordenadoria de Epidemiologia, Barra Mansa, Rio de Janeiro. Documentos retirados do SINANNET/07-05-2008.
-
22.
Instituto Oswaldo Cruz, 2011. Esquistossomose no Rio de Janeiro é tema de encontro. [Comunicação].
-
23.
Hoffman WA, Pons JA, Janer JL, 1934. The sedimentation-concentration method in schistosomiasis mansoni. Puerto Rico J Publ Hlth 9: 283–291.
-
24.
Kirkwood B, Sterne JA, 2003. Essential Medical Statistics. Second edition. Maldan, MA: Blackwell Publishing, 512.
-
25.
Kato K, Miura M, 1954. Comparative examinations. Kisechugaku Zasshi 3: 35.
-
26.
Noya OB, de Noya A, Losada S, Colmenares C, Guzmán C, Lorenzo MA, Bermúdez H, 2002. Laboratory diagnosis of schistosomiasis in areas of low transmission: a review of a line of research. Mem Inst Oswaldo Cruz 97: 167–169.
-
27.
Silva RM, Silva MI, Vellosa SA, Garcia ET, Kanamura HY, 1992. Pesquisa de anticorpos IgM contra tubo digestivo do verme para diagnóstico da esquistossomose mansônica. Rev Bras Pat Clin 28: 39–42.
-
28.
Ruiz JM, 1952. Cercaria species indexes of Schistosoma mansoni verified in Neves and Mariana, State of Minas Gerais. Mem Inst Butantan 24: 63–65.
-
29.
Pellegrino J, Siqueira AF, 1956. A perfusion technic for recovery of Schistosoma mansoni from experimentally infected guinea pig. Rev Bras Malariol Doencas Trop 4: 589–597.
-
30.
Deelder AM, Kornelis D, Van Marck EA, Eveleigh PC, Van Egmond JG, 1980. Schistosoma mansoni: characterization of two circulating polysaccharide antigens and the immunological response to these antigens in mouse, hamster, and human infections. Exp Parasitol 50: 16–32.
-
31.
Dresden MH, Payne DC, 1981. A sieving method for the collection of schistosome eggs from mouse intestines. J Parasitol 3: 450–452.
-
32.
Pinto PL, Floriano LD, Ferreira SC, Suto LM, Vellosa SA, 1995. Purificação de ovos de Schistosoma mansoni a partir de vísceras de hamsters (Crycetus auratus) experimentalmente infectados. XIV Congresso da Sociedade Brasileira de Parasitologia; 1995; Goiânia, BR. Rev Patol Trop 23: 264.
-
33.
Prata A, 1970. Como se caracteriza a forma hepatoesplênica da esquistossomose? In: Prata AS, Aboim E, eds. II Simpósio sobre Esquistossomose, Diretoria de Saúde Marinha - Universidade Federal da Bahia. Salvador, 179–184.
-
34.
Zhu YC, 2005. Immunodiagnosis and its role in schistosomiasis control in China: a review. Acta Trop 96: 130–139.
-
35.
Wanga W, Youzi L, Hongjun L, Yuntian X, Guoli Q, Jianrong D, Yousheng L, 2012. Immunodiagnostic efficacy of detection of Schistosoma japonicum human infections in China: a meta analysis. Asian Pac J Trop Med 5: 15–23.
-
36.
Oliveira EJ, Kanamura HY, Lima DM, 2005. Efficacy of an enzyme-linked immunosorbent assay as a diagnostic tool for schistosomiasis mansoni in individuals with low worm burden. Mem Inst Oswaldo Cruz 100: 421–425.
-
37.
Oliveira LM, Santos HL, Goncalves MM, Barreto MG, Peralta JM, 2010. Evaluation of polymerase chain reaction as an additional tool for the diagnosis of low-intensity Schistosoma mansoni infection. Diagn Microbiol Infect Dis 68: 416–421.
-
38.
Sackett DL, Strauss SE, Richardson WR, Rosenberg W, Haynes RB, 2003. Medicina baseada em evidências: prática e ensino. Second edition. Porto Alegre: Artmed.
-
39.
Mello SG, 2011. Epidemiologia da esquistossomose e conhecimento da população em área periurbana de Sergipe [dissertação]. Universidade de Tiradentes - UNIT. Aracaju.
-
40.
de Noya BA, Ruiz R, Losada S, Colmenares C, Contreras R, Cesari IM, Noya O, 2007. Detection of schistosomiasis cases in low-transmission areas based on coprologic and serologic criteria: the Venezuelan experience. Acta Trop 103: 41–49.
-
41.
Sackett DL, Straus SE, Richardson WS, Rosemberg WM, Haynes RB, 2000. Evidence-based Medicine, How to Practice and Teach EBM. Second edition. London, UK: Churchill Livingstone.
-
42.
Vendrame CM, Carvalho MD, Yamamoto CR, Nakhle MC, Carvalho SA, Chieffi PP, 2001. Evaluation of anti-Schistosoma mansoni IgG antibodies in patients with chronic schistosomiasis mansoni before and after specific treatment. Rev Inst Med Trop Sao Paulo 43: 153–159.
-
43.
Kloetzel K, 1959. A reação de precipitação periovular na esquistossomose. II. Correlação aos dados clínicos. Rev Inst Med Trop Sao Paulo 1: 129–137.
-
44.
Mello RT, 1976. A reação periovular no diagnóstico e controle de cura da esquistossomose mansoni [tese]. Belo Horizonte: Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas.
-
45.
Coker CM, Oliver-Gonzalez J, 1957. Experiments on anti-schistosome circumoval precipitating antibody in mice. Am J Trop Med Hyg 7: 390.
-
46.
Senterfit LB, 1958. Immobilization of the miracidia of Schistosoma mansoni by immune sera. II. The occurrence of the antibody during the course of schistosomiasis. Am J Trop Med Hyg 68: 148–155.
-
47.
Oliver-Gonzalez J, Bauman PM, Benenson AS, 1955. Immunological aspects of infections with Schistosoma mansoni. Am J Trop Med Hyg 4: 443–452.
-
48.
Cheeve AW, 1968. A quantitative post-mortem study of schistosomiasis mansoni in man. Am J Trop Med Hyg 17: 38–64.
-
49.
de Vlas SJ, Gryseels B, 1992. Underestimation of Schistosoma mansoni prevalence. Parasitol Today 8: 274–277.
-
50.
Engels D, Sinzinkayo E, De Vlas SJ, Gryseels B, 1997. Intraspecimen fecal egg count variation in Schistosoma mansoni infection. Am J Trop Med Hyg 5: 571–577.
-
51.
Ye XP, Donnelly CA, Anderson RM, Fu YL, Agnew A, 1998. The distribution of Schistosoma japonicum eggs in feces and the effect of stirring fecal specimens. Ann Trop Med Parasitol 92: 181–185.
-
52.
Correa-Oliveira R, Dusse LM, Viana IR, Colley DG, Carvalho OS, Gazzinelli G, 1988. Human antibody responses against schistosomal antigens, I. Antibodies from patients with Ancylostoma, Ascaris lumbricoides or Schistosoma mansoni infections react with schistosome antigens. Am J Trop Med Hyg 2: 348–355.
-
53.
Sathe BD, Pandit CH, Chanderkar NG, Badade DC, Sengupta SR, Renapurkar DM, 1991. Sero-diagnosis of schistosomiasis by ELISA test in an endemic area of Gimvi village, India. Am J Trop Med Hyg 94: 76–78.
-
54.
Smith H, Doenhoff M, Aitken C, Bailey W, Ji M, Dawson E, Gilis H, Spence G, Alexander C, van Gool T, 2012. Comparison of Schistosoma mansoni soluble cercarial antigens and soluble egg antigens for serodiagnosing schistosome infections. PLoS Negl Trop Dis 6: e1815.
-
55.
Enk MJ, Lima AC, Drummond SC, Schall VT, Coelho PM, 2008. The impact of the number of stool samples on the prevalence, the infection intensity and the distribution of the infection with Schistosoma mansoni among a population in an area of low transmission. Acta Trop 108: 222–228.
-
56.
Zhang YY, Luo JP, Liu YM, Wang QZ, Chen JH, Xu MX, Xu JM, Wu J, Tu XM, Wu GL, Zhang ZS, Wu HW, 2009. Evaluation of Kato-Katz examination method in three areas with low-level endemicity of schistosomiasis japonica in China: a Bayesian modeling approach. Acta Trop 112: 16–22.
-
57.
Noya BA, Noya O, Balzan C, Cesari IM, 1992. New approaches for the control and eradication of schistosomiasis in Venezuela. Mem Inst Oswaldo Cruz 87: 227–231.
-
58.
Grenfell RF, Martins W, Enk M, Almeida A, Siqueira L, Silva-Moraes V, Oliveira E, Carneiro NF, Coelho PM, 2013. Schistosoma mansoni in a low-prevalence area in Brazil: the importance of additional methods for the diagnosis of hard-to-detect individual carriers by low-cost immunological assays. Mem Inst Oswaldo Cruz 108: 328–334.
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Detection of Schistosoma mansoni Antibodies in a Low-Endemicity Area Using Indirect Immunofluorescence and Circumoval Precipitin Test
Maria Cristina Carvalho do Espírito-Santo
Maria Cristina Carvalho do Espírito-Santo
Department of Infectious and Parasitic Diseases, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Department of Enteroparasites at the Parasitology and Mycology, Instituto Adolfo Lutz, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Faculdade de Medicina da Universiddae de São Paulo, Brazil; Laboratory of Clinical Parasitology of the Central Laboratory, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil
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Pedro Luiz Pinto
Pedro Luiz Pinto
Department of Infectious and Parasitic Diseases, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Department of Enteroparasites at the Parasitology and Mycology, Instituto Adolfo Lutz, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Faculdade de Medicina da Universiddae de São Paulo, Brazil; Laboratory of Clinical Parasitology of the Central Laboratory, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil
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Cybele Gargioni
Cybele Gargioni
Department of Infectious and Parasitic Diseases, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Department of Enteroparasites at the Parasitology and Mycology, Instituto Adolfo Lutz, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Faculdade de Medicina da Universiddae de São Paulo, Brazil; Laboratory of Clinical Parasitology of the Central Laboratory, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil
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Monica Viviana Alvarado-Mora
Monica Viviana Alvarado-Mora
Department of Infectious and Parasitic Diseases, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Department of Enteroparasites at the Parasitology and Mycology, Instituto Adolfo Lutz, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Faculdade de Medicina da Universiddae de São Paulo, Brazil; Laboratory of Clinical Parasitology of the Central Laboratory, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil
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Vera Lúcia Pagliusi Castilho
Vera Lúcia Pagliusi Castilho
Department of Infectious and Parasitic Diseases, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Department of Enteroparasites at the Parasitology and Mycology, Instituto Adolfo Lutz, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Faculdade de Medicina da Universiddae de São Paulo, Brazil; Laboratory of Clinical Parasitology of the Central Laboratory, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil
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João Ranato Rebello Pinho
João Ranato Rebello Pinho
Department of Infectious and Parasitic Diseases, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Department of Enteroparasites at the Parasitology and Mycology, Instituto Adolfo Lutz, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Faculdade de Medicina da Universiddae de São Paulo, Brazil; Laboratory of Clinical Parasitology of the Central Laboratory, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil
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Expedito José de Albuquerque Luna
Expedito José de Albuquerque Luna
Department of Infectious and Parasitic Diseases, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Department of Enteroparasites at the Parasitology and Mycology, Instituto Adolfo Lutz, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Faculdade de Medicina da Universiddae de São Paulo, Brazil; Laboratory of Clinical Parasitology of the Central Laboratory, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil
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Ronaldo Cesar Borges Gryschek
Ronaldo Cesar Borges Gryschek
Department of Infectious and Parasitic Diseases, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Department of Enteroparasites at the Parasitology and Mycology, Instituto Adolfo Lutz, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Faculdade de Medicina da Universiddae de São Paulo, Brazil; Laboratory of Clinical Parasitology of the Central Laboratory, Hospital das Clínicas da Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil; Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, Instituto de Medicina Tropical de São Paulo, Universidade de São Paulo, São Paulo, Brazil
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Parasitological diagnostic methods for schistosomiasis lack sensitivity, especially in regions of low endemicity. The objective of this study was to determine the prevalence of Schistosoma mansoni infections by antibody detection using the indirect immunofluorescence assay (IFA-IgM) and circumoval precipitin test (COPT). Serum samples of 572 individuals were randomly selected. The IFA-IgM and COPT were used to detect anti-S. mansoni antibodies. Of the patients studied, 15.9% (N = 91) were IFA-IgM positive and 5.1% (N = 29) had COPT reactions (P < 0.001 by McNemar's test). Immunodiagnostic techniques showed higher infection prevalence than had been previously estimated. This study suggests that combined use of these diagnostic tools could be useful for the diagnosis of schistosomiasis in epidemiological studies in areas of low endemicity.
Author Notes
Authors' addresses: Maria Cristina Espírito-Santo, Department of Infectious and Parasitic Diseases, Faculdade de Medicina da Universidade de São Paulo, São Paulo, Brazil, E-mail: cristinasanto@usp.br. Pedro Luiz Pinto, Adolfo Lutz Institut, Department of Enteroparasites at the Parasitology, São Paulo, São Paulo, Brazil, E-mail: pedrp.luiz44@terra.com.br. Cybele Gargioni, Adolfo Lutz Institiute, Department of Enteroparasites at the Parasitology and Mycology, São Paulo, São Paulo, Brazil, E-mail: cgargioni@gmail.com. Mónica Viviana Alvarado-Mora, School of Medicine, University of São Paulo, Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, São Paulo, São Paulo, Brazil, E-mail: monica.viviana@usp.br. Vera Lúcia Pagliusi Castilho, Faculdade de Medicina da Universidade de São Paulo, Laboratory of Clinical Parasitology of the Central Laboratory of the Hosiital das Clinicas, São Paulo, São Paulo, Brazil, E-mail: vera.castilho@hc.fm.usp.br. João Ranato Rebello Pinho, Faculdade de Medicina da Universidade de São Paulo, Laboratory of Tropical Gastroenterology and Hepatology, Department of Gastroenterology, São Paulo, São Paulo, Brazil, E-mail: jrrpinho@usp.br. Expedito José de Albuquerque Luna, Universidade de São Paulo, Instituto de Medicina Tropical de São Paulo, São Paulo, São Paulo, Brazil, E-mail: eluna@usp.br. Ronaldo Cesar Borges Gryschek, Faculdade de Medicina da Universidade de São Paulo, Department of Infectious and Parasitic Diseases, São Paulo, São Paulo, Brazil, E-mail: rebgry@usp.br.
- Introduction
- Methods
- Statistical analysis.
- Ethical aspects.
- Ethical aspects of animal experiments.
- Methods of laboratory diagnosis.
- Parasitological methods.
- Laboratory maintenance of the S. mansoni experimental cycle.
- Periovular reaction.
- Detection of IgM antibodies against antigens of the S. mansoni digestive tract.
- Antibody detection of S. mansoni egg antigens.
- Results
- Discussion
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
World Health Organization, 2001. Prevention and Control of Schistosomiasis and the Soil-Transmitted Helminthiasis. Report of a WHO Expert Committee. Geneva: WHO, 73.
-
2.
Chitsulo L, Engels D, Montresor A, Savioli L, 2000. The global status of schistosomiasis and its control. Acta Trop 77: 41–51.
-
3.
World Health Organization, 2001. Report of Informal Consultation on Schistosomiasis in Low Transmission Areas: Control Strategies and Criteria for Elimination. London, 10–13 April 2000. Geneva: WHO, 52.
-
4.
Alarcón de Noya BA, Guevara RR, Colmenares C, Losada S, Noya O, 2006. Low transmission areas of schistosomiasis in Venezuela: consequences on the diagnosis, treatment, and control. Mem Inst Oswaldo Cruz 101: 29–35.
-
5.
Ministério da Saúde, 2012. Secretaria de Vigilância a Saúde. Sistema de Informação do Programa de Controle da Esquistossomose. Sistema de Informação de Agravos em Saúde/2012. Casos confirmados de Esquistossomose, Brasil, Grandes Regiões e Unidades Federadas, 1995 a 2011. Brasil. (Dados preliminares, sujeitos à revisão).
-
6.
São Paulo, 2007. Secretaria de Estado da Saúde. Centro de Vigilância Epidemiológica. Coordenadoria de Controle de Doenças. Vigilância Epidemiológica e Controle da Esquistossomose-Normas e Instruções. São Paulo, 45.
-
7.
Katz N, Chaves A, Pellegrino J, 1972. A simple device for quantitative stool thick smear technique in schistosomiasis mansoni. Rev Inst Med Trop Sao Paulo 14: 397–400.
-
8.
Kongs A, Marks G, Verle P, Van der Stuyft P, 2001. The unreliability of the Kato-Katz technique limits its usefulness for evaluating S. mansoni infections. Trop Med Int Health 6: 163–169.
-
9.
Engels D, Sinzinkayo E, Gryseels B, 1996. Day-to-day egg count fluctuation in Schistosoma mansoni infection and its operational implications. Am J Trop Med Hyg 54: 319–324.
-
10.
Teesdale CH, Fahringer K, Chistulo L, 1985. Egg count variation and sensitivity of a thin smear technique for the diagnosis of Schistosoma mansoni. Trans R Soc Trop Med Hyg 79: 369–373.
-
11.
World Health Organization, 2012. Schistosomiasis. Fact Sheet No. 115. Geneva: WHO.
-
12.
Teles HM, de Carvalho ME, Santos Ferreira C, Zacharias F, de Lima VR, Fadel ML, 2002. Schistosomiasis mansoni in Bananal (State of São Paulo, Brazil): I. Efficiency of diagnostic and treatment procedures. Mem Inst Oswaldo Cruz 97: 181–186.
-
13.
Alarcón de Noya BA, Noya O, Balzan C, Cesari IM, 1992. New approaches for the control and eradication of schistosomiasis in Venezuela. Mem Inst Oswaldo Cruz 87: 227–231.
-
14.
Wang W, Li Y, Li H, Xing Y, Qu G, Dai J, Liang Y, 2012. Immunodiagnostic efficacy of detection of Schistosoma japonicum human infections in China: a meta analysis. Asian Pac J Trop Med 5: 15–23.
-
15.
Kanamura HY, Dias LC, da Silva RM, Glasser CM, Patucci RM, Vellosa SA, Antunes JL, 1998. A comparative epidemiologic study of specific antibodies (IgM and IgA) and parasitological findings in an endemic area of low transmission of Schistosoma mansoni. Rev Inst Med Trop Sao Paulo 40: 85–91.
-
16.
Burlandy-Soares LC, de Souza Dias LC, Kanamura HY, de Oliveira EJ, Ciaravolo RM, 2003. Schistosomiasis mansoni: follow-up of control program based on parasitologic and serologic methods in a Brazilian community of low endemicity. Mem Inst Oswaldo Cruz 98: 853–859.
-
17.
Oliver-Gonzalez J, 1954. Anti-egg precipitins in the serum of humans infected with Schistosoma mansoni. J Infect Dis 95: 86–91.
-
18.
Spencer L, Alarcón de Noya B, Noya O, Masroua G, 1991. Comparative analysis between the circumoval precipitin test and ELISA with raw antigens for the diagnosis of schistosomiasis in Venezuela. G E N 45: 77–83 Spanish.
-
19.
Hillyer GV, Tiben ER, Knight WB, Gómez de Rios I, Pelley RP, 1979. Immunodiagnosis of infection with Schistosoma mansoni: comparison of ELISA, radioimmunoassay, and precipitation tests performed with antigens from eggs. Am J Trop Med Hyg 28: 661–669.
-
20.
Rio de Janeiro, 2011. Pactuação das Ações de Vigilância em Saúde. Pactuação das ações prioritárias da PAVS para o biênio 2010–2011. Monitorar situação epidemiológica da esquistossomose nos municípios das áreas endêmicas do Rio de Janeiro: Porciúncula, Paracambi, Sumidouro, Barra Mansa, Duas Barras e Rio de Janeiro.
-
21.
Rio de Janeiro, 2008. Secretaria Municipal de Saúde, Coordenadoria de Epidemiologia, Barra Mansa, Rio de Janeiro. Documentos retirados do SINANNET/07-05-2008.
-
22.
Instituto Oswaldo Cruz, 2011. Esquistossomose no Rio de Janeiro é tema de encontro. [Comunicação].
-
23.
Hoffman WA, Pons JA, Janer JL, 1934. The sedimentation-concentration method in schistosomiasis mansoni. Puerto Rico J Publ Hlth 9: 283–291.
-
24.
Kirkwood B, Sterne JA, 2003. Essential Medical Statistics. Second edition. Maldan, MA: Blackwell Publishing, 512.
-
25.
Kato K, Miura M, 1954. Comparative examinations. Kisechugaku Zasshi 3: 35.
-
26.
Noya OB, de Noya A, Losada S, Colmenares C, Guzmán C, Lorenzo MA, Bermúdez H, 2002. Laboratory diagnosis of schistosomiasis in areas of low transmission: a review of a line of research. Mem Inst Oswaldo Cruz 97: 167–169.
-
27.
Silva RM, Silva MI, Vellosa SA, Garcia ET, Kanamura HY, 1992. Pesquisa de anticorpos IgM contra tubo digestivo do verme para diagnóstico da esquistossomose mansônica. Rev Bras Pat Clin 28: 39–42.
-
28.
Ruiz JM, 1952. Cercaria species indexes of Schistosoma mansoni verified in Neves and Mariana, State of Minas Gerais. Mem Inst Butantan 24: 63–65.
-
29.
Pellegrino J, Siqueira AF, 1956. A perfusion technic for recovery of Schistosoma mansoni from experimentally infected guinea pig. Rev Bras Malariol Doencas Trop 4: 589–597.
-
30.
Deelder AM, Kornelis D, Van Marck EA, Eveleigh PC, Van Egmond JG, 1980. Schistosoma mansoni: characterization of two circulating polysaccharide antigens and the immunological response to these antigens in mouse, hamster, and human infections. Exp Parasitol 50: 16–32.
-
31.
Dresden MH, Payne DC, 1981. A sieving method for the collection of schistosome eggs from mouse intestines. J Parasitol 3: 450–452.
-
32.
Pinto PL, Floriano LD, Ferreira SC, Suto LM, Vellosa SA, 1995. Purificação de ovos de Schistosoma mansoni a partir de vísceras de hamsters (Crycetus auratus) experimentalmente infectados. XIV Congresso da Sociedade Brasileira de Parasitologia; 1995; Goiânia, BR. Rev Patol Trop 23: 264.
-
33.
Prata A, 1970. Como se caracteriza a forma hepatoesplênica da esquistossomose? In: Prata AS, Aboim E, eds. II Simpósio sobre Esquistossomose, Diretoria de Saúde Marinha - Universidade Federal da Bahia. Salvador, 179–184.
-
34.
Zhu YC, 2005. Immunodiagnosis and its role in schistosomiasis control in China: a review. Acta Trop 96: 130–139.
-
35.
Wanga W, Youzi L, Hongjun L, Yuntian X, Guoli Q, Jianrong D, Yousheng L, 2012. Immunodiagnostic efficacy of detection of Schistosoma japonicum human infections in China: a meta analysis. Asian Pac J Trop Med 5: 15–23.
-
36.
Oliveira EJ, Kanamura HY, Lima DM, 2005. Efficacy of an enzyme-linked immunosorbent assay as a diagnostic tool for schistosomiasis mansoni in individuals with low worm burden. Mem Inst Oswaldo Cruz 100: 421–425.
-
37.
Oliveira LM, Santos HL, Goncalves MM, Barreto MG, Peralta JM, 2010. Evaluation of polymerase chain reaction as an additional tool for the diagnosis of low-intensity Schistosoma mansoni infection. Diagn Microbiol Infect Dis 68: 416–421.
-
38.
Sackett DL, Strauss SE, Richardson WR, Rosenberg W, Haynes RB, 2003. Medicina baseada em evidências: prática e ensino. Second edition. Porto Alegre: Artmed.
-
39.
Mello SG, 2011. Epidemiologia da esquistossomose e conhecimento da população em área periurbana de Sergipe [dissertação]. Universidade de Tiradentes - UNIT. Aracaju.
-
40.
de Noya BA, Ruiz R, Losada S, Colmenares C, Contreras R, Cesari IM, Noya O, 2007. Detection of schistosomiasis cases in low-transmission areas based on coprologic and serologic criteria: the Venezuelan experience. Acta Trop 103: 41–49.
-
41.
Sackett DL, Straus SE, Richardson WS, Rosemberg WM, Haynes RB, 2000. Evidence-based Medicine, How to Practice and Teach EBM. Second edition. London, UK: Churchill Livingstone.
-
42.
Vendrame CM, Carvalho MD, Yamamoto CR, Nakhle MC, Carvalho SA, Chieffi PP, 2001. Evaluation of anti-Schistosoma mansoni IgG antibodies in patients with chronic schistosomiasis mansoni before and after specific treatment. Rev Inst Med Trop Sao Paulo 43: 153–159.
-
43.
Kloetzel K, 1959. A reação de precipitação periovular na esquistossomose. II. Correlação aos dados clínicos. Rev Inst Med Trop Sao Paulo 1: 129–137.
-
44.
Mello RT, 1976. A reação periovular no diagnóstico e controle de cura da esquistossomose mansoni [tese]. Belo Horizonte: Universidade Federal de Minas Gerais, Instituto de Ciências Biológicas.
-
45.
Coker CM, Oliver-Gonzalez J, 1957. Experiments on anti-schistosome circumoval precipitating antibody in mice. Am J Trop Med Hyg 7: 390.
-
46.
Senterfit LB, 1958. Immobilization of the miracidia of Schistosoma mansoni by immune sera. II. The occurrence of the antibody during the course of schistosomiasis. Am J Trop Med Hyg 68: 148–155.
-
47.
Oliver-Gonzalez J, Bauman PM, Benenson AS, 1955. Immunological aspects of infections with Schistosoma mansoni. Am J Trop Med Hyg 4: 443–452.
-
48.
Cheeve AW, 1968. A quantitative post-mortem study of schistosomiasis mansoni in man. Am J Trop Med Hyg 17: 38–64.
-
49.
de Vlas SJ, Gryseels B, 1992. Underestimation of Schistosoma mansoni prevalence. Parasitol Today 8: 274–277.
-
50.
Engels D, Sinzinkayo E, De Vlas SJ, Gryseels B, 1997. Intraspecimen fecal egg count variation in Schistosoma mansoni infection. Am J Trop Med Hyg 5: 571–577.
-
51.
Ye XP, Donnelly CA, Anderson RM, Fu YL, Agnew A, 1998. The distribution of Schistosoma japonicum eggs in feces and the effect of stirring fecal specimens. Ann Trop Med Parasitol 92: 181–185.
-
52.
Correa-Oliveira R, Dusse LM, Viana IR, Colley DG, Carvalho OS, Gazzinelli G, 1988. Human antibody responses against schistosomal antigens, I. Antibodies from patients with Ancylostoma, Ascaris lumbricoides or Schistosoma mansoni infections react with schistosome antigens. Am J Trop Med Hyg 2: 348–355.
-
53.
Sathe BD, Pandit CH, Chanderkar NG, Badade DC, Sengupta SR, Renapurkar DM, 1991. Sero-diagnosis of schistosomiasis by ELISA test in an endemic area of Gimvi village, India. Am J Trop Med Hyg 94: 76–78.
-
54.
Smith H, Doenhoff M, Aitken C, Bailey W, Ji M, Dawson E, Gilis H, Spence G, Alexander C, van Gool T, 2012. Comparison of Schistosoma mansoni soluble cercarial antigens and soluble egg antigens for serodiagnosing schistosome infections. PLoS Negl Trop Dis 6: e1815.
-
55.
Enk MJ, Lima AC, Drummond SC, Schall VT, Coelho PM, 2008. The impact of the number of stool samples on the prevalence, the infection intensity and the distribution of the infection with Schistosoma mansoni among a population in an area of low transmission. Acta Trop 108: 222–228.
-
56.
Zhang YY, Luo JP, Liu YM, Wang QZ, Chen JH, Xu MX, Xu JM, Wu J, Tu XM, Wu GL, Zhang ZS, Wu HW, 2009. Evaluation of Kato-Katz examination method in three areas with low-level endemicity of schistosomiasis japonica in China: a Bayesian modeling approach. Acta Trop 112: 16–22.
-
57.
Noya BA, Noya O, Balzan C, Cesari IM, 1992. New approaches for the control and eradication of schistosomiasis in Venezuela. Mem Inst Oswaldo Cruz 87: 227–231.
-
58.
Grenfell RF, Martins W, Enk M, Almeida A, Siqueira L, Silva-Moraes V, Oliveira E, Carneiro NF, Coelho PM, 2013. Schistosoma mansoni in a low-prevalence area in Brazil: the importance of additional methods for the diagnosis of hard-to-detect individual carriers by low-cost immunological assays. Mem Inst Oswaldo Cruz 108: 328–334.
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