Blood-Meal Hosts of the Enzootic Vector of Eastern Equine Encephalomyelitis Virus, Culiseta melanura, in Michigan, United States

John B. Keven Department of Microbiology, Genetics, and Immunology, Michigan State University, East Lansing, Michigan;
Vector-Borne Diseases Unit, Papua New Guinea Institute of Medical Research, Madang, Papua New Guinea

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Edward D. Walker Department of Microbiology, Genetics, and Immunology, Michigan State University, East Lansing, Michigan;

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Eastern equine encephalomyelitis virus (EEEV) is endemic in Michigan, showing an upsurge in human cases and in infections of white-tailed deer, horses, and other animals in the past decade (2010–2020). However, blood-host associations of the enzootic mosquito vector Culiseta melanura in the Great Lakes region are poorly known compared with other better-studied regions. Vertebrate sources of blood meals of Cs. melanura collected from resting boxes were determined through sequencing of the mitochondrial cytochrome B gene generated from polymerase chain reaction. Thirty-six unique avian species were detected in the samples, and 42% of the blood meals originated from only two species (American robin and northern cardinal). This result shows that although the Cs. melanura population investigated here used a wide range of avian hosts, American robin and northern cardinal are the main hosts in southwestern Michigan.

Author Notes

Financial support: This study was supported by the U.S. CDC (Grant no. U01 CK000651) through a subcontract from the University of Wisconsin to Michigan State University. The funder had no role in the study design, data collection, analysis, and interpretation of results. The contents of this publication are solely the responsibility of the authors and do not necessarily represent the official views of the CDC or the Department of Health and Human Services.

Current contact information: John B. Keven, Vector-Borne Diseases Unit, Papua New Guinea Institute of Medical Research, Madang, Papua New Guinea, E-mail: john.keven@pngimr.org.pg. Edward D. Walker, Department of Microbiology, Genetics, and Immunology, Michigan State University, East Lansing, MI, E-mail: walker@msu.edu.

Address correspondence to John B. Keven, Biomedical and Physical Sciences Bldg., Rm. 6179, 567 Wilson Rd., Michigan State University, East Lansing, MI 48824. E-mail: jbkeven@gmail.com
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