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1.
Chomel BB, Henn JB, Kasten RW, Nieto NC, Foley J, Papageorgiou S, Allen C, Koehler JE, 2009. Dogs are more permissive than cats or Guinea pigs to experimental infection with a human isolate of Bartonella rochalimae. Vet Res 40: 27.
-
2.
Eremeeva ME et al. 2007. Bacteremia, fever, and splenomegaly caused by a newly recognized Bartonella species. N Engl J Med 356: 2381–2387.
-
3.
Henn JB, Chomel BB, Boulouis HJ, Kasten RW, Murray WJ, Bar-Gal GK, King R, Courreau JF, Baneth G, 2009. Bartonella rochalimae in raccoons, coyotes, and red foxes. Emerg Infect Dis 15: 1984–1987.
-
4.
Diniz PPVP, Billeter SA, Otranto D, De Caprariis D, Petanides T, Mylonakis ME, Koutinas AF, Breitschwerdt EB, 2009. Molecular documentation of Bartonella infection in dogs in Greece and Italy. J Clin Microbiol 47: 1565–1567.
-
5.
Diniz PPVP et al. 2013. Infection of domestic dogs in Peru by zoonotic Bartonella species: a cross-sectional prevalence study of 219 asymptomatic dogs. PLoS Negl Trop Dis 7: e2393.
-
6.
Billeter SA, Gundi VA, Rood MP, Kosoy MY, 2011. Molecular detection and identification of Bartonella species in Xenopsylla cheopis fleas (Siphonaptera: Pulicidae) collected from Rattus norvegicus rats in Los Angeles, California. Appl Environ Microbiol 77: 7850–7852.
-
7.
Billeter SA, Caceres AG, Gonzales-Hidalgo J, Luna-Caypo D, Kosoy MY, 2011. Molecular detection of Bartonella species in ticks from Peru. J Med Entomol 48: 1257–1260.
-
8.
Pulliainen AT, Dehio C, 2012. Persistence of Bartonella spp. stealth pathogens: from subclinical infections to vasoproliferative tumor formation. FEMS Microbiol Rev 36: 563–599.
-
9.
La Scola B, Zeaiter Z, Khamis A, Raoult D, 2003. Gene-sequence-based criteria for species definition in bacteriology: the Bartonella paradigm. Trends Microbiol 11: 318–321.
-
10.
Qurollo BA, Riggins D, Comyn A, Zewde MT, Breitschwerdt EB, 2014. Development and validation of a sensitive and specific sodB-based quantitative PCR assay for molecular detection of Ehrlichia species. J Clin Microbiol 52: 4030–4032.
-
11.
U.S. Department of Energy Office of Biological and Environmental Research, 2016. JGI/IMG—Integrated Microbial Genomes & Microbiome Samples. Available at: https://img.jgi.doe.gov/cgi-bin/m/main.cgi. Accessed November 11, 2016.
-
12.
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ, 1990. Basic local alignment search tool. J Mol Biol 215: 403–410.
-
13.
Wattam AR et al. 2017. Improvements to PATRIC, the all-bacterial bioinformatics database and analysis resource center. Nucleic Acids Res 45: D535–D542.
-
14.
Edgar RC, 2004. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32: 1792–1797.
-
15.
Shenkin PS, Erman B, Mastrandrea LD, 1991. Information-theoretical entropy as a measure of sequence variability. Proteins 11: 297–313.
-
16.
Oksanen J et al. 2017. Ordination Methods, Diversity Analysis and Other Functions for Community and Vegetation Ecologists. Available at: https://cran.r-project.org/web/packages/vegan/index.html. Accessed June 1, 2017.
-
17.
Wickham H, 2016. Use R! ggplot2: Elegant Graphics for Data Analysis. New York, NY: Springer.
-
18.
Cherry NA, Maggi RG, Cannedy AL, Breitschwerdt EB, 2009. PCR detection of Bartonella bovis and Bartonella henselae in the blood of beef cattle. Vet Microbiol 135: 308–312.
-
19.
Diniz PPVP, Maggi RG, Schwartz DS, Cadenas MB, Bradley JM, Hegarty B, Breitschwerdt EB, 2007. Canine bartonellosis: serological and molecular prevalence in Brazil and evidence of co-infection with Bartonella henselae and Bartonella vinsonii subsp. berkhoffii. Vet Res 38: 697–710.
-
20.
Breitschwerdt EB, Maggi RG, Chomel BB, Lappin MR, 2010. Bartonellosis: an emerging infectious disease of zoonotic importance to animals and human beings. J Vet Emerg Crit Care (San Antonio) 20: 8–30.
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Bartonella rochalimae Detection by a Sensitive and Specific PCR Platform
Dennis Chan
Dennis Chan
College of Veterinary Medicine, Western University of Health Sciences, Pomona, California;
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Joseph Andrew Geiger
Joseph Andrew Geiger
Graduate College of Biomedical Sciences, Western University of Health Sciences, Pomona, California
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Elton José Rosas Vasconcelos
Elton José Rosas Vasconcelos
College of Veterinary Medicine, Western University of Health Sciences, Pomona, California;
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Brian Oakley
Brian Oakley
College of Veterinary Medicine, Western University of Health Sciences, Pomona, California;
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Pedro Paulo Vissotto de Paiva Diniz
Pedro Paulo Vissotto de Paiva Diniz
College of Veterinary Medicine, Western University of Health Sciences, Pomona, California;
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Bartonella rochalimae is an emerging zoonotic pathogen present in the United States, South America, and Europe. The molecular detection of B. rochalimae frequently relies on polymerase chain reaction (PCR) assays that target the genus Bartonella coupled with DNA sequencing for species determination. However, the presence of other Bartonella spp. in the sample being tested may result in false-negative results for B. rochalimae, especially when Sanger sequencing is used. We developed a sensitive and specific quantitative PCR platform for B. rochalimae by targeting the intergenic transcribed spacer, gltA, and rpoB genes, which are recommended for subtyping characterization. This PCR platform achieved the limit of detection between five and 10 genomic equivalents per reaction and did not amplify DNA from other Bartonella species or selected hosts. This PCR platform is a fast and cost-effective option to be used in epidemiological evaluations of reservoirs and vectors and in detecting and quantifying B. rochalimae infection in humans.
- Supplemental Materials (XLSX 33 KB)
Author Notes
Financial support: D. C. reports grants and non-financial support from Merial (Boehringer Ingelheim) during the conduct of the study. P. P. V. P. D. reports grants and personal fees from Merial (Boehringer Ingelheim outside the submitted work.
Authors’ addresses: Dennis Chan, Elton José Rosas Vasconcelos, Brian Oakley, and Pedro Paulo Vissotto de Paiva Diniz, College of Veterinary Medicine, Western University of Health Sciences, Pomona, CA, E-mails: chand@westernu.edu, evasconcelos@westernu.edu, boakley@westernu.edu, and pdiniz@westernu.edu. Joseph Andrew Geiger, Graduate College of Biomedical Sciences, Western University of Health Sciences, Pomona, CA, E-mail: joseph.geiger@westernu.edu.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Chomel BB, Henn JB, Kasten RW, Nieto NC, Foley J, Papageorgiou S, Allen C, Koehler JE, 2009. Dogs are more permissive than cats or Guinea pigs to experimental infection with a human isolate of Bartonella rochalimae. Vet Res 40: 27.
-
2.
Eremeeva ME et al. 2007. Bacteremia, fever, and splenomegaly caused by a newly recognized Bartonella species. N Engl J Med 356: 2381–2387.
-
3.
Henn JB, Chomel BB, Boulouis HJ, Kasten RW, Murray WJ, Bar-Gal GK, King R, Courreau JF, Baneth G, 2009. Bartonella rochalimae in raccoons, coyotes, and red foxes. Emerg Infect Dis 15: 1984–1987.
-
4.
Diniz PPVP, Billeter SA, Otranto D, De Caprariis D, Petanides T, Mylonakis ME, Koutinas AF, Breitschwerdt EB, 2009. Molecular documentation of Bartonella infection in dogs in Greece and Italy. J Clin Microbiol 47: 1565–1567.
-
5.
Diniz PPVP et al. 2013. Infection of domestic dogs in Peru by zoonotic Bartonella species: a cross-sectional prevalence study of 219 asymptomatic dogs. PLoS Negl Trop Dis 7: e2393.
-
6.
Billeter SA, Gundi VA, Rood MP, Kosoy MY, 2011. Molecular detection and identification of Bartonella species in Xenopsylla cheopis fleas (Siphonaptera: Pulicidae) collected from Rattus norvegicus rats in Los Angeles, California. Appl Environ Microbiol 77: 7850–7852.
-
7.
Billeter SA, Caceres AG, Gonzales-Hidalgo J, Luna-Caypo D, Kosoy MY, 2011. Molecular detection of Bartonella species in ticks from Peru. J Med Entomol 48: 1257–1260.
-
8.
Pulliainen AT, Dehio C, 2012. Persistence of Bartonella spp. stealth pathogens: from subclinical infections to vasoproliferative tumor formation. FEMS Microbiol Rev 36: 563–599.
-
9.
La Scola B, Zeaiter Z, Khamis A, Raoult D, 2003. Gene-sequence-based criteria for species definition in bacteriology: the Bartonella paradigm. Trends Microbiol 11: 318–321.
-
10.
Qurollo BA, Riggins D, Comyn A, Zewde MT, Breitschwerdt EB, 2014. Development and validation of a sensitive and specific sodB-based quantitative PCR assay for molecular detection of Ehrlichia species. J Clin Microbiol 52: 4030–4032.
-
11.
U.S. Department of Energy Office of Biological and Environmental Research, 2016. JGI/IMG—Integrated Microbial Genomes & Microbiome Samples. Available at: https://img.jgi.doe.gov/cgi-bin/m/main.cgi. Accessed November 11, 2016.
-
12.
Altschul SF, Gish W, Miller W, Myers EW, Lipman DJ, 1990. Basic local alignment search tool. J Mol Biol 215: 403–410.
-
13.
Wattam AR et al. 2017. Improvements to PATRIC, the all-bacterial bioinformatics database and analysis resource center. Nucleic Acids Res 45: D535–D542.
-
14.
Edgar RC, 2004. MUSCLE: multiple sequence alignment with high accuracy and high throughput. Nucleic Acids Res 32: 1792–1797.
-
15.
Shenkin PS, Erman B, Mastrandrea LD, 1991. Information-theoretical entropy as a measure of sequence variability. Proteins 11: 297–313.
-
16.
Oksanen J et al. 2017. Ordination Methods, Diversity Analysis and Other Functions for Community and Vegetation Ecologists. Available at: https://cran.r-project.org/web/packages/vegan/index.html. Accessed June 1, 2017.
-
17.
Wickham H, 2016. Use R! ggplot2: Elegant Graphics for Data Analysis. New York, NY: Springer.
-
18.
Cherry NA, Maggi RG, Cannedy AL, Breitschwerdt EB, 2009. PCR detection of Bartonella bovis and Bartonella henselae in the blood of beef cattle. Vet Microbiol 135: 308–312.
-
19.
Diniz PPVP, Maggi RG, Schwartz DS, Cadenas MB, Bradley JM, Hegarty B, Breitschwerdt EB, 2007. Canine bartonellosis: serological and molecular prevalence in Brazil and evidence of co-infection with Bartonella henselae and Bartonella vinsonii subsp. berkhoffii. Vet Res 38: 697–710.
-
20.
Breitschwerdt EB, Maggi RG, Chomel BB, Lappin MR, 2010. Bartonellosis: an emerging infectious disease of zoonotic importance to animals and human beings. J Vet Emerg Crit Care (San Antonio) 20: 8–30.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 632 | 483 | 35 |
| Full Text Views | 841 | 18 | 2 |
| PDF Downloads | 191 | 12 | 0 |