Maroli M, Feliciangeli D, Bichaud L, Charrel RN, Gradoni L, 2013. Phlebotomine sandflies and spreading of leishmaniases and other diseases of public health concern. Med Vet Entomol 27: 123–147.
King AM, Adams MJ, Caestens EB, Lefkowitz EJ, 2012. Virus Taxonomy: Ninth Report of the International Committee on Taxonomy of Viruses. Amsterdam: Elsevier.
Depaquit J, Grandadam M, Fouque F, Andry PE, Peyrefitte C, 2010. Arthropod-borne viruses transmitted by Phletomine sandflies in Europe: a review. Euro Surveill 15: 19507.
Ciufolini MG, Maroli M, Miceli N, Nicoletti L, Cecchetti S, Amore R, Percopo S, Verani P, 1990. Monitoring of Phlebotominae sandflies transmitted viruses activity in Marche region (Italy). Arbo Info Exch 58–59.
Charrel RN, Gallian P, Navarro-Mari J-M, Nicoletti L, Papa A, Sánchez-Seco MP, Tenorio A, de Lamballerie X, 2005. Emergence of Toscana virus in Europe. Emerg Infect Dis 1: 1657–1663.
Venturi G, Madeddu G, Rezza G, Ciccozzi M, Pettinato ML, Cilliano M, Fiorentini C, Mura MS, Ciufolini MG, 2007. Detection of Toscana virus central nervous system infections in Sardinia Island, Italy. J Clin Virol 40: 90–91.
Theodor O, 1958. Psychodidae-Phlebotomine. Lindner E, ed. Die Fliegen der Palaearktischen Region, 9c. Stuttgart, Germany: Schweizerbart'sche Verlagsbuchhandlung, 1–55.
Léger N, Pesson B, Madulo-Leblond G, Abonnenc E, 1983. Differentiation of females of the subgenus Larroussius Nitzulescu 1931 (Diptera-Phlebotomidae) of the Mediterranean region. Ann Parasitol Hum Comp 58: 611–623.
Charrel RN, Izri A, Temmam S, Delaunay P, Toga I, Dumon H, Marty P, de Lamballerie X, Parola P, 2007. Cocirculation of 2 genotypes of Toscana virus, southeastern France. Emerg Infect Dis 13: 465–468.
Sánchez-Seco MP, EchevarrÃa JM, Hernández L, Estévez D, Navarro-Marà JM, Tenorio AJ, 2003. Detection and identification of Toscana and other phleboviruses by RT-nested-PCR assays with degenerated primers. Med Virol 71: 140–149.
Verani P, Ciufolini MG, Caciolli S, Renzi A, Nicoletti L, Sabatinelli G, Bartolozzi D, Volpi G, Amaducci L, Coluzzi M, 1988. Ecology of viruses isolated from sand flies in Italy and characterized of a new Phlebovirus (Arbia virus). Am J Trop Med Hyg 38: 433–439.
Ciufolini MG, Maroli M, Guandalini E, Marchi A, Verani P, 1989. Experimental studies on the maintenance of Toscana and Arbia viruses (Bunyaviridae: Phlebovirus). Am J Trop Med Hyg 40: 669–675.
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Thirty pools from 900 (540 females and 360 males) Phlebotomus perfiliewi sandflies collected during the summer of 2012 in the Fermo area (Marche Region, central Italy) were tested for the presence of Phleboviruses. A nested polymerase chain reaction was performed using degenerated primers amplifying a fragment of the polymerase gene (large segment) and a fragment of the nucleoprotein gene (small segment) of the genus Phlebovirus. One pool was positive for Toscana virus, as expected from results of studies in the area, and six pools were positive for a putative novel Phlebovirus. Virus isolation in Vero cells was performed. Minimum field infection rates/1,000 insects processed for the novel and Toscana viruses were 6.7 and 1.0, respectively. Phylogenetic analysis of the novel Phlebovirus, tentatively named Fermo virus, placed it in the Sandfly Fever Naples virus serocomplex.
Financial support: This study was supported by European Union grant FP7-261504 EDENext.
Authors' addresses: Maria Elena Remoli, Claudia Fortuna, Antonella Marchi, Paola Bucci, and Maria Grazia Ciufolini, Unit of Viral Diseases and Attenuated Vaccines, Department of Infectious, Parasitic and Immunomediated Disease, Istituto Superiore Di Sanità , Rome, Italy, E-mails: mariaelena.remoli@iss.it, claudia.fortuna@iss.it, antonella.marchi@iss.it, paola.bucci@iss.it, and mariagrazia.ciufolini@iss.it. Claudio Argentini, Global Health Unit, Department of Therapeutic Research and Medicines Evaluation, Istituto Superiore di Sanità , Rome, Italy, E-mail: claudio.argentini@iss.it. Gioia Bongiorno, Michele Maroli, Luigi Gradoni, and Marina Gramiccia, Unit of Vector-Borne Diseases and International Health, Department of Infectious, Parasitic and Immunomediated Disease, Istituto Superiore di Sanità , Rome, Italy, E-mails: gioia.bongiorno@iss.it, michele.maroli@gmail.com, luigi.gradoni@iss.it, and marina.gramiccia@iss.it.
Maroli M, Feliciangeli D, Bichaud L, Charrel RN, Gradoni L, 2013. Phlebotomine sandflies and spreading of leishmaniases and other diseases of public health concern. Med Vet Entomol 27: 123–147.
King AM, Adams MJ, Caestens EB, Lefkowitz EJ, 2012. Virus Taxonomy: Ninth Report of the International Committee on Taxonomy of Viruses. Amsterdam: Elsevier.
Depaquit J, Grandadam M, Fouque F, Andry PE, Peyrefitte C, 2010. Arthropod-borne viruses transmitted by Phletomine sandflies in Europe: a review. Euro Surveill 15: 19507.
Ciufolini MG, Maroli M, Miceli N, Nicoletti L, Cecchetti S, Amore R, Percopo S, Verani P, 1990. Monitoring of Phlebotominae sandflies transmitted viruses activity in Marche region (Italy). Arbo Info Exch 58–59.
Charrel RN, Gallian P, Navarro-Mari J-M, Nicoletti L, Papa A, Sánchez-Seco MP, Tenorio A, de Lamballerie X, 2005. Emergence of Toscana virus in Europe. Emerg Infect Dis 1: 1657–1663.
Venturi G, Madeddu G, Rezza G, Ciccozzi M, Pettinato ML, Cilliano M, Fiorentini C, Mura MS, Ciufolini MG, 2007. Detection of Toscana virus central nervous system infections in Sardinia Island, Italy. J Clin Virol 40: 90–91.
Theodor O, 1958. Psychodidae-Phlebotomine. Lindner E, ed. Die Fliegen der Palaearktischen Region, 9c. Stuttgart, Germany: Schweizerbart'sche Verlagsbuchhandlung, 1–55.
Léger N, Pesson B, Madulo-Leblond G, Abonnenc E, 1983. Differentiation of females of the subgenus Larroussius Nitzulescu 1931 (Diptera-Phlebotomidae) of the Mediterranean region. Ann Parasitol Hum Comp 58: 611–623.
Charrel RN, Izri A, Temmam S, Delaunay P, Toga I, Dumon H, Marty P, de Lamballerie X, Parola P, 2007. Cocirculation of 2 genotypes of Toscana virus, southeastern France. Emerg Infect Dis 13: 465–468.
Sánchez-Seco MP, EchevarrÃa JM, Hernández L, Estévez D, Navarro-Marà JM, Tenorio AJ, 2003. Detection and identification of Toscana and other phleboviruses by RT-nested-PCR assays with degenerated primers. Med Virol 71: 140–149.
Verani P, Ciufolini MG, Caciolli S, Renzi A, Nicoletti L, Sabatinelli G, Bartolozzi D, Volpi G, Amaducci L, Coluzzi M, 1988. Ecology of viruses isolated from sand flies in Italy and characterized of a new Phlebovirus (Arbia virus). Am J Trop Med Hyg 38: 433–439.
Ciufolini MG, Maroli M, Guandalini E, Marchi A, Verani P, 1989. Experimental studies on the maintenance of Toscana and Arbia viruses (Bunyaviridae: Phlebovirus). Am J Trop Med Hyg 40: 669–675.
Past two years | Past Year | Past 30 Days | |
---|---|---|---|
Abstract Views | 275 | 248 | 33 |
Full Text Views | 351 | 8 | 1 |
PDF Downloads | 90 | 6 | 1 |