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Characterization of Novel Leishmania infantum Recombinant Proteins Encoded by Genes from Five Families with Distinct Capacities for Serodiagnosis of Canine and Human Visceral Leishmaniasis
Geraldo G. S. Oliveira
Geraldo G. S. Oliveira
Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Bahia, Brazil; Instituto Nacional de Doenças Tropicais, Salvador, Bahia, Brazil; Centro de Pesquisas Aggeu Magalhães, Recife, Pernambuco, Brazil
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Franklin B. Magalhães
Franklin B. Magalhães
Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Bahia, Brazil; Instituto Nacional de Doenças Tropicais, Salvador, Bahia, Brazil; Centro de Pesquisas Aggeu Magalhães, Recife, Pernambuco, Brazil
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Márcia C. A. Teixeira
Márcia C. A. Teixeira
Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Bahia, Brazil; Instituto Nacional de Doenças Tropicais, Salvador, Bahia, Brazil; Centro de Pesquisas Aggeu Magalhães, Recife, Pernambuco, Brazil
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Andrea M. Pereira
Andrea M. Pereira
Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Bahia, Brazil; Instituto Nacional de Doenças Tropicais, Salvador, Bahia, Brazil; Centro de Pesquisas Aggeu Magalhães, Recife, Pernambuco, Brazil
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Cristiane G. M. Pinheiro
Cristiane G. M. Pinheiro
Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Bahia, Brazil; Instituto Nacional de Doenças Tropicais, Salvador, Bahia, Brazil; Centro de Pesquisas Aggeu Magalhães, Recife, Pernambuco, Brazil
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Lenita R. Santos
Lenita R. Santos
Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Bahia, Brazil; Instituto Nacional de Doenças Tropicais, Salvador, Bahia, Brazil; Centro de Pesquisas Aggeu Magalhães, Recife, Pernambuco, Brazil
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Marília B. Nascimento
Marília B. Nascimento
Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Bahia, Brazil; Instituto Nacional de Doenças Tropicais, Salvador, Bahia, Brazil; Centro de Pesquisas Aggeu Magalhães, Recife, Pernambuco, Brazil
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Cheila N. G. Bedor
Cheila N. G. Bedor
Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Bahia, Brazil; Instituto Nacional de Doenças Tropicais, Salvador, Bahia, Brazil; Centro de Pesquisas Aggeu Magalhães, Recife, Pernambuco, Brazil
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Alessandra L. Albuquerque
Alessandra L. Albuquerque
Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Bahia, Brazil; Instituto Nacional de Doenças Tropicais, Salvador, Bahia, Brazil; Centro de Pesquisas Aggeu Magalhães, Recife, Pernambuco, Brazil
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Washington L. C. dos-Santos
Washington L. C. dos-Santos
Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Bahia, Brazil; Instituto Nacional de Doenças Tropicais, Salvador, Bahia, Brazil; Centro de Pesquisas Aggeu Magalhães, Recife, Pernambuco, Brazil
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Yara M. Gomes
Yara M. Gomes
Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Bahia, Brazil; Instituto Nacional de Doenças Tropicais, Salvador, Bahia, Brazil; Centro de Pesquisas Aggeu Magalhães, Recife, Pernambuco, Brazil
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Edson D. Moreira Jr
Edson D. Moreira Jr
Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Bahia, Brazil; Instituto Nacional de Doenças Tropicais, Salvador, Bahia, Brazil; Centro de Pesquisas Aggeu Magalhães, Recife, Pernambuco, Brazil
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Maria E. F. Brito
Maria E. F. Brito
Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Bahia, Brazil; Instituto Nacional de Doenças Tropicais, Salvador, Bahia, Brazil; Centro de Pesquisas Aggeu Magalhães, Recife, Pernambuco, Brazil
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Lain C. Pontes de Carvalho
Lain C. Pontes de Carvalho
Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Bahia, Brazil; Instituto Nacional de Doenças Tropicais, Salvador, Bahia, Brazil; Centro de Pesquisas Aggeu Magalhães, Recife, Pernambuco, Brazil
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Osvaldo P. de Melo Neto
Osvaldo P. de Melo Neto
Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Salvador, Bahia, Brazil; Instituto Nacional de Doenças Tropicais, Salvador, Bahia, Brazil; Centro de Pesquisas Aggeu Magalhães, Recife, Pernambuco, Brazil
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To expand the available panel of recombinant proteins that can be useful for identifying Leishmania-infected dogs and for diagnosing human visceral leishmaniasis (VL), we selected recombinant antigens from L. infantum, cDNA, and genomic libraries by using pools of serum samples from infected dogs and humans. The selected DNA fragments encoded homologs of a cytoplasmic heat-shock protein 70, a kinesin, a polyubiquitin, and two novel hypothetical proteins. Histidine-tagged recombinant proteins were produced after subcloning these DNA fragments and evaluated by using an enzyme-linked immunosorbent assays with panels of canine and human serum samples. The enzyme-linked immunosorbent assays with different recombinant proteins had different sensitivities (67.4–93.0% and 36.4–97.2%) and specificities (76.1–100% and 90.4–97.3%) when tested with serum samples from Leishmania-infected dogs and human patients with VL. Overall, no single recombinant antigen was sufficient to serodiagnosis all canine or human VL cases.
Author Notes
Financial support: This study was supported by the Programa Rede Nordeste de Biotecnologia (RENORBIO); BNB-FINEP-MCT, Brazil; PDTIS (RID17), FIOCRUZ, Brazil; and Instituto Nacional de Ciencia e Tecnologia de Doenças Tropicais, CNPq (INCT-DT grant 573839/2008-5).
Authors' addresses: Geraldo G. S. Oliveira, Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Rua Waldemar Falcão, No. 121, Candeal, CEP 40.296-710, Salvador, Bahia, Brazil, and Instituto Nacional de Ciencia e Tecnologia de Doenças Tropicais, Salvador, Bahia, Brazil, E-mail: ggileno@bahia.fiocruz.br. Márcia C. A. Teixeira, Andrea M. Pereira, Cristiane G. M. Pinheiro, Lenita R. Santos, Washington L. C. dos-Santos, Edson D. Moreira Jr, and Lain C. Pontes de Carvalho, Centro de Pesquisas Gonçalo Moniz, Fundação Oswaldo Cruz, Av. Waldemar Falcão, No. 121, Brotas, CEP 40.296-710, Salvador, Bahia, Brazil. Franklin B. Magalhães, Marília B. Nascimento, Cheila N. G. Bedor, Alessandra L. Albuquerque, Yara M. Gomes, Maria E. F. Brito, and Osvaldo P. Melo Neto, Centro de Pesquisas Aggeu Magalhães, Fiocruz, Av. Moraes Rego s/n, Campus UFPE, CEP 50670-420, Recife, Pernambuco, Brazil.
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