Short report: a rapid method for the differentiation of Burkholderia pseudomallei and Burkholderia thailandensis.

Vanaporn Wuthiekanun Faculty of Tropical Medicine and Faculty of Science, Mahidol University, Bangkok, Thailand.

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Narisara Anuntagool Faculty of Tropical Medicine and Faculty of Science, Mahidol University, Bangkok, Thailand.

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Nicholas J White Faculty of Tropical Medicine and Faculty of Science, Mahidol University, Bangkok, Thailand.

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Stitaya Sirisinha Faculty of Tropical Medicine and Faculty of Science, Mahidol University, Bangkok, Thailand.

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DOI:
https://doi.org/10.4269/ajtmh.2002.66.759
Volume/Issue:
Volume 66: Issue 6
Page(s):
759–761
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A rapid method for the identification and differentiation of Burkholderia pseudomallei and Burkholderia thailandensis colonies is described. It consists of simultaneous use of 2 monoclonal antibody-based latex agglutination test systems. The anti-lipopolysaccharide test reacts with both species, whereas the anti-exopolysaccharide reacts only with B. pseudomallei. Compared with classical biochemical tests, the method is highly reproducible and accurate. It is particularly useful for the identification of the organisms in environmental specimens, which may contain both of these Burkholderia species.

Author Notes

Published Online:
Jun 2002
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
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