| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 388 | 333 | 44 |
| Full Text Views | 483 | 11 | 1 |
| PDF Downloads | 206 | 18 | 1 |
Rickettsial phospholipase A2 as a pathogenic mechanism in a model of cell injury by typhus and spotted fever group rickettsiae.
D H Walker
D H Walker
Department of Pathology, University of Texas Medical Branch, Galveston 77555-0609, USA.
Search for other papers by D H Walker in
Current site
Google Scholar
PubMed
Search for other papers by D H Walker in
Current site
Google Scholar
PubMed
H M Feng
H M Feng
Department of Pathology, University of Texas Medical Branch, Galveston 77555-0609, USA.
Search for other papers by H M Feng in
Current site
Google Scholar
PubMed
Search for other papers by H M Feng in
Current site
Google Scholar
PubMed
V L Popov
V L Popov
Department of Pathology, University of Texas Medical Branch, Galveston 77555-0609, USA.
Search for other papers by V L Popov in
Current site
Google Scholar
PubMed
Search for other papers by V L Popov in
Current site
Google Scholar
PubMed
Phospholipase A2 activity by typhus group rickettsiae causes hemolysis in vitro. Rickettsial phospholipase A2 has been proposed to mediate entry into the host cell, escape from the phagosome, and cause injury to host cells by both typhus and spotted fever group rickettsiae. In a rickettsial contact-associated cytotoxicity model, the interaction of Rickettsia prowazekii or R. conorii with Vero cells caused temperature-dependent release of 51Cr from the cells. Treatment of rickettsiae, but not the cells, with a phospholipase A2 inhibitor (bromophenacyl bromide) or with antibody to king cobra venom inhibited cell injury. Rickettsial treatment with bromophenacyl bromide inhibited the release of free fatty acids from the host cell. Neither the inhibitor nor antivenom impaired rickettsial active transport of L-lysine. Thus, host cell injury was mediated by a rickettsial phospholipase A2-dependent mechanism.
Author Notes
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 388 | 333 | 44 |
| Full Text Views | 483 | 11 | 1 |
| PDF Downloads | 206 | 18 | 1 |