| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 202 | 99 | 12 |
| Full Text Views | 152 | 1 | 0 |
| PDF Downloads | 38 | 3 | 0 |
Analysis of mefloquine resistance and amplification of pfmdr1 in multidrug-resistant Plasmodium falciparum isolates from Thailand.
S C Chaiyaroj
S C Chaiyaroj
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Search for other papers by S C Chaiyaroj in
Current site
Google Scholar
PubMed
Search for other papers by S C Chaiyaroj in
Current site
Google Scholar
PubMed
A Buranakiti
A Buranakiti
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Search for other papers by A Buranakiti in
Current site
Google Scholar
PubMed
Search for other papers by A Buranakiti in
Current site
Google Scholar
PubMed
P Angkasekwinai
P Angkasekwinai
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Search for other papers by P Angkasekwinai in
Current site
Google Scholar
PubMed
Search for other papers by P Angkasekwinai in
Current site
Google Scholar
PubMed
S Looressuwan
S Looressuwan
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Search for other papers by S Looressuwan in
Current site
Google Scholar
PubMed
Search for other papers by S Looressuwan in
Current site
Google Scholar
PubMed
A F Cowman
A F Cowman
Department of Microbiology, Faculty of Science, Mahidol University, Bangkok, Thailand.
Search for other papers by A F Cowman in
Current site
Google Scholar
PubMed
Search for other papers by A F Cowman in
Current site
Google Scholar
PubMed
Resistance to quinoline-containing compound has been associated with the Plasmodium falciparum multidrug resistance 1 (pfmdr1) gene. We analyzed wild P. falciparum isolates with high levels of chloroquine and mefloquine resistance for their macrorestriction maps of chromosome 5 and sequence of pfmdr1. Two types of chromosome 5 amplification were found. Eleven of 62 resistant isolates displayed Bgl 1 fragments larger than 100 kb. Twenty-nine isolates possessed multiple copies of the fragments. We failed to detect any amplification of this region on chromosome 5 in 22 mefloquine-resistant isolates, suggesting that other mechanisms can mediate the mefloquine-resistant phenotype. There was no direct association between pfmdr1 mutations and chloroquine sensitivity. Resistant lines could have Asn-86 and Tyr-184 or Phe-184, the predicted sequence of those chloroquine-sensitive isolates. No mutation at Asn-1042 and Asp-1246 was detected among these chloroquine-resistant isolates. Therefore, a few base substitutions in the pfmdr1 gene may not be sufficient to account for all chloroquine-resistant phenotypes.
Author Notes
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 202 | 99 | 12 |
| Full Text Views | 152 | 1 | 0 |
| PDF Downloads | 38 | 3 | 0 |