Short Report: Isolation and Partial Characterization of a Puumala Virus from a Human Case of Nephropathia Epidemica in France

Pierre E. Rollin Centre National de Reference pour les Fievres Hemorragiques Virales, Institut Pasteur, Special Pathogens Branch, Centers for Disease Control and Prevention, Department of Veterinary Public Health, Faculty of Veterinary Medicine, Hokkaido University, Service de Medecine Interne, Hopital d'Instruction des Armees Desgenettes, Institut Merieux, Paris, France

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Michael D. Bowen Centre National de Reference pour les Fievres Hemorragiques Virales, Institut Pasteur, Special Pathogens Branch, Centers for Disease Control and Prevention, Department of Veterinary Public Health, Faculty of Veterinary Medicine, Hokkaido University, Service de Medecine Interne, Hopital d'Instruction des Armees Desgenettes, Institut Merieux, Paris, France

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Hiroaki Kariwa Centre National de Reference pour les Fievres Hemorragiques Virales, Institut Pasteur, Special Pathogens Branch, Centers for Disease Control and Prevention, Department of Veterinary Public Health, Faculty of Veterinary Medicine, Hokkaido University, Service de Medecine Interne, Hopital d'Instruction des Armees Desgenettes, Institut Merieux, Paris, France

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Jean-Francois Saluzzo Centre National de Reference pour les Fievres Hemorragiques Virales, Institut Pasteur, Special Pathogens Branch, Centers for Disease Control and Prevention, Department of Veterinary Public Health, Faculty of Veterinary Medicine, Hokkaido University, Service de Medecine Interne, Hopital d'Instruction des Armees Desgenettes, Institut Merieux, Paris, France

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Sylvain Guerard Centre National de Reference pour les Fievres Hemorragiques Virales, Institut Pasteur, Special Pathogens Branch, Centers for Disease Control and Prevention, Department of Veterinary Public Health, Faculty of Veterinary Medicine, Hokkaido University, Service de Medecine Interne, Hopital d'Instruction des Armees Desgenettes, Institut Merieux, Paris, France

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Alain Flechaire Centre National de Reference pour les Fievres Hemorragiques Virales, Institut Pasteur, Special Pathogens Branch, Centers for Disease Control and Prevention, Department of Veterinary Public Health, Faculty of Veterinary Medicine, Hokkaido University, Service de Medecine Interne, Hopital d'Instruction des Armees Desgenettes, Institut Merieux, Paris, France

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Daniel Coudrier Centre National de Reference pour les Fievres Hemorragiques Virales, Institut Pasteur, Special Pathogens Branch, Centers for Disease Control and Prevention, Department of Veterinary Public Health, Faculty of Veterinary Medicine, Hokkaido University, Service de Medecine Interne, Hopital d'Instruction des Armees Desgenettes, Institut Merieux, Paris, France

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Pierre Sureau Centre National de Reference pour les Fievres Hemorragiques Virales, Institut Pasteur, Special Pathogens Branch, Centers for Disease Control and Prevention, Department of Veterinary Public Health, Faculty of Veterinary Medicine, Hokkaido University, Service de Medecine Interne, Hopital d'Instruction des Armees Desgenettes, Institut Merieux, Paris, France

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C. J. Peters Centre National de Reference pour les Fievres Hemorragiques Virales, Institut Pasteur, Special Pathogens Branch, Centers for Disease Control and Prevention, Department of Veterinary Public Health, Faculty of Veterinary Medicine, Hokkaido University, Service de Medecine Interne, Hopital d'Instruction des Armees Desgenettes, Institut Merieux, Paris, France

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Stuart T. Nichol Centre National de Reference pour les Fievres Hemorragiques Virales, Institut Pasteur, Special Pathogens Branch, Centers for Disease Control and Prevention, Department of Veterinary Public Health, Faculty of Veterinary Medicine, Hokkaido University, Service de Medecine Interne, Hopital d'Instruction des Armees Desgenettes, Institut Merieux, Paris, France

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DOI:
https://doi.org/10.4269/ajtmh.1995.52.577
Volume/Issue:
Volume 52: Issue 6
Page(s):
577–578
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Puumala (PUU) virus (Bunyaviridae: Hantavirus), the etiologic agent of nephropathia epidemica (NE), the mild form of hemorrhagic fever with renal syndrome, is enzootic in Europe and has been known to occur in France since 1983. We report the first isolation of PUU virus in France and western Europe from a case of NE acquired in France. The virus was isolated from a serum collected in the acute phase of the clinical course by successive blind passages in Vero E6 cells. Serologic typing using monoclonal antibodies confirmed the identity of the virus as PUU. The sequence of an 832-nucleotide fragment of the virus medium RNA segment obtained by the polymerase chain reaction (PCR) also classified it as a PUU virus. The sequence of this isolate from a human case in France is closely related to the sequence of a PUU virus obtained by the PCR from a German patient.

Author Notes

Copyright:
Copyright © 1995 by The American Society of Tropical Medicine and Hygiene 1995
Published Online:
Jun 1995
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
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