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Acridine Orange Fluorescent Microscopy and the Detection of Malaria in Populations with Low-Density Parasitemia
Chansuda Wongsrichanalai
Chansuda Wongsrichanalai
AFRIMS, Queen Saovabdha Memorial Institute, The Thai Red Cross Society, Pra Pokklao Provincial Hospital, Ministry of Public Health, Program for Appropriate Technology in Health (DiaTech), Bangkok, Thailand
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Jaturaporn Pornsilapatip
Jaturaporn Pornsilapatip
AFRIMS, Queen Saovabdha Memorial Institute, The Thai Red Cross Society, Pra Pokklao Provincial Hospital, Ministry of Public Health, Program for Appropriate Technology in Health (DiaTech), Bangkok, Thailand
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Vittayut Namsiripongpun
Vittayut Namsiripongpun
AFRIMS, Queen Saovabdha Memorial Institute, The Thai Red Cross Society, Pra Pokklao Provincial Hospital, Ministry of Public Health, Program for Appropriate Technology in Health (DiaTech), Bangkok, Thailand
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H. Kyle Webster
H. Kyle Webster
AFRIMS, Queen Saovabdha Memorial Institute, The Thai Red Cross Society, Pra Pokklao Provincial Hospital, Ministry of Public Health, Program for Appropriate Technology in Health (DiaTech), Bangkok, Thailand
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Anna Luccini
Anna Luccini
AFRIMS, Queen Saovabdha Memorial Institute, The Thai Red Cross Society, Pra Pokklao Provincial Hospital, Ministry of Public Health, Program for Appropriate Technology in Health (DiaTech), Bangkok, Thailand
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Pongpun Pansamdang
Pongpun Pansamdang
AFRIMS, Queen Saovabdha Memorial Institute, The Thai Red Cross Society, Pra Pokklao Provincial Hospital, Ministry of Public Health, Program for Appropriate Technology in Health (DiaTech), Bangkok, Thailand
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Henry Wilde
Henry Wilde
AFRIMS, Queen Saovabdha Memorial Institute, The Thai Red Cross Society, Pra Pokklao Provincial Hospital, Ministry of Public Health, Program for Appropriate Technology in Health (DiaTech), Bangkok, Thailand
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Malinee Prasittisuk
Malinee Prasittisuk
AFRIMS, Queen Saovabdha Memorial Institute, The Thai Red Cross Society, Pra Pokklao Provincial Hospital, Ministry of Public Health, Program for Appropriate Technology in Health (DiaTech), Bangkok, Thailand
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Detection of low-density malaria parasites with Giemsa-stained thick smears (G-TS) requires time and experience and becomes impractical with high sample loads. Acridine orange fluorescent microscopy (AO/FM) of capillary centrifuged blood may offer an alternative technique. We compared AO/FM readings with G-TS in 290 specimens from asymptomatic people in Thai villages endemic for malaria. AO/FM specimens were prepared in modified capillary tubes coated with acridine orange (Quantitative Buffy Coat or “QBC tubes”) and examined under a fluorescent microscope. Twenty-three (85.2%) of the 27 specimens found positive by G-TS had under 100 parasites/µl blood (< 35 parasites/200 microscopic fields). The overall AO/FM sensitivity was 78.9% [range: 66.7% (10/15) – 86.7% (13/15)]. For Plasmodium falciparum, regardless of stages, the sensitivities varied from 66.7% (8/12) to 91.7% (11/12). AO/FM performed better for P. falciparum than for Plasmodium vivax and for asexual than for sexual stages of the parasite. However, the species- and stage-specific results must be interpreted with caution because of the small sample sizes and very low parasite densities involved. The test specificity was 96.6% [range: 95.6% (263/275)–97.1% (263/271)]. These levels of accuracy plus the known advantages of AO/FM suggest that the test, supplemented with G-TS to improve species and stage differentiation, is also useful for screening low-density parasitemias.
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| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 867 | 435 | 37 |
| Full Text Views | 28 | 2 | 1 |
| PDF Downloads | 32 | 4 | 2 |