Preparation of an Attenuated Dengue 4 (341750 Carib) Virus Vaccine

II. Safety and Immunogenicity in Humans

Charles H. Hoke Jr. Walter Reed Army Institute of Research, U.S. Army Research Institute of Infectious Diseases, Washington, DC

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Frank J. Malinoski Walter Reed Army Institute of Research, U.S. Army Research Institute of Infectious Diseases, Washington, DC

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Kenneth H. Eckels Walter Reed Army Institute of Research, U.S. Army Research Institute of Infectious Diseases, Washington, DC

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Robert McNair Scott Walter Reed Army Institute of Research, U.S. Army Research Institute of Infectious Diseases, Washington, DC

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Doria R. Dubois Walter Reed Army Institute of Research, U.S. Army Research Institute of Infectious Diseases, Washington, DC

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Peter L. Summers Walter Reed Army Institute of Research, U.S. Army Research Institute of Infectious Diseases, Washington, DC

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Thomas Simms Walter Reed Army Institute of Research, U.S. Army Research Institute of Infectious Diseases, Washington, DC

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Jeanne Burrous Walter Reed Army Institute of Research, U.S. Army Research Institute of Infectious Diseases, Washington, DC

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Sherman E. Hasty Walter Reed Army Institute of Research, U.S. Army Research Institute of Infectious Diseases, Washington, DC

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William H. Bancroft Walter Reed Army Institute of Research, U.S. Army Research Institute of Infectious Diseases, Washington, DC

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DOI:
https://doi.org/10.4269/ajtmh.1990.43.219
Volume/Issue:
Volume 43: Issue 2
Page(s):
219–226
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To determine safety and immunogenicity, a single 0.5 ml dose of a monovalent live-attenuated dengue (DEN) 4 (341750 Carib) vaccine was given sc to 3 groups of flavivirus nonimmune volunteers in increasing concentrations. Two recipients received 103 plaque forming units (PFU)/dose (1:100 dilution of stock vaccine). One remained asymptomatic, but became viremic between days 12 and 15, experienced a mild elevation of temperature (37.4°C), and developed DEN-4 specific antibody. Neither recipient of the 104 PFU became infected. Eight volunteers then received undiluted vaccine (105 PFU). Viremia and antibody (neutralizing, hemagglutination inhibition, and IgM) developed in 5 of the 8 (63%). These 5 volunteers also developed a scarcely noticeable macular, blanching rash and minimal temperature elevations (37.3, 38.1, 37, 37.9, and 37.9°C). Clinically insignificant decreases in total white blood cell, lymphocyte, and polymorphonuclear cell counts and an elevation in mononuclear cell counts occurred in association with viremia. This vaccine is safe, reasonably immunogenic, and suitable for further evaluation.

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Copyright:
Copyright © 1990 by The American Society of Tropical Medicine and Hygiene 1990
Published Online:
Aug 1990
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
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