Dengue-2 Vaccine: Infection of Aedes aegypti Mosquitoes by Feeding on Viremic Recipients

William H. Bancroft Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, Washington, D.C. 20012

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Robert McN. Scott Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, Washington, D.C. 20012

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Walter E. Brandt Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, Washington, D.C. 20012

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Jack M. McCown Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, Washington, D.C. 20012

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Kenneth H. Eckels Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, Washington, D.C. 20012

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David E. Hayes Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, Washington, D.C. 20012

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Douglas J. Gould Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, Washington, D.C. 20012

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Philip K. Russell Division of Communicable Diseases and Immunology, Walter Reed Army Institute of Research, Washington, D.C. 20012

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DOI:
https://doi.org/10.4269/ajtmh.1982.31.1229
Volume/Issue:
Volume 31: Issue 6
Page(s):
1229–1231
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Abstract. Colonized Aedes aegypti mosquitoes were fed on voluntary recipients of an experimental, live, attenuated, dengue type 2 (PR-159/S-1) vaccine to estimate the frequency of vector infection and the stability of the virus in mosquitoes. Two volunteers were viremic at the time of mosquito feeding, but only two of 114 mosquitoes that took a viremic blood meal became infected with the vaccine virus. Strains of virus recovered from the bodies of the mosquitoes and the volunteer's blood retained the temperature sensitivity and small plaque growth characteristics of the vaccine virus. Dengue viral antigen was not detectable in any of the mosquito heads by direct immunofluorescence and in vitro virus transmission by droplet feeding was not observed. This experiment showed that vector mosquitoes can be infected with vaccine virus by feeding on viremic vaccinees. Furthermore, the virus is sufficiently stable to retain the in vitro growth characteristics associated with the vaccine virus.

Author Notes

Copyright:
Copyright © 1982 by The American Society of Tropical Medicine and Hygiene 1982
Accepted:
07 Apr 1982
|
Published Online:
Nov 1982
Cover The American Journal of Tropical Medicine and Hygiene
The American Journal of Tropical Medicine and Hygiene
Print ISSN:
0002-9637
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