Laboratory Studies of Transovarial Transmission of Trivittatus Virus by Aedes Trivittatus

Bruce M. Christensen Department of Entomology, Iowa State University, State Hygienic Laboratory, University of Iowa, Ames, Iowa 50011

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Wayne A. Rowley Department of Entomology, Iowa State University, State Hygienic Laboratory, University of Iowa, Ames, Iowa 50011

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Yau W. Wong Department of Entomology, Iowa State University, State Hygienic Laboratory, University of Iowa, Ames, Iowa 50011

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Donald C. Dorsey Department of Entomology, Iowa State University, State Hygienic Laboratory, University of Iowa, Ames, Iowa 50011

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William J. Hausler Jr. Department of Entomology, Iowa State University, State Hygienic Laboratory, University of Iowa, Ames, Iowa 50011

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Aedes trivittatus were experimentally exposed through a membrane to a concentration of trivittatus (TVT) virus in dog defibrinated blood of 103.4–103.5 SMICLD50/0.025 ml. Transovarial and transstadial transmission was demonstrated. Fifty-five percent of the infected mosquitoes tested transovarially transmitted TVT virus. TVT virus was isolated from 16% of the F1 generation progeny examined, including 19% of the larvae, 23% of adult females, and 10% of adult males.

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