Author:
ProCite
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Reference Manager
BibTeX
Zotero
EndNote
-
1.
Mas-Coma S, Valero MA, Bargues MD, 2022. Human and animal fascioliasis: Origins and worldwide evolving scenario. Clin Microbiol Rev 35: e0008819.
-
2.
Bui TD, Doanh PN, Saegerman C, Losson B, 2016. Current status of fasciolosis in Vietnam: An update and perspectives. J Helminthol 90: 511–522.
-
3.
Mas-Coma S, 2004. Human fascioliasis. Cotruvo JA, Dufour A, Rees G, eds. Waterborne Zoonoses: Identification, Causes, and Control. London, United Kingdom: World Health Organization, International Water Association Publishing, 305–322.
-
4.
Mas-Coma S, Adela Valero M, Dolores Bargues M, 2015. Fasciola and fasciolopsis. Xiao L, Ryan U, Feng Y, eds. Biology of Food Borne Parasites. 1st ed. New York, NY: Taylor and Francis Group, 371–404.
-
5.
Cwiklinski K, O’Neill SM, Donnelly S, Dalton JP, 2016. A prospective view of animal and human fasciolosis. Parasite Immunol 38: 558–568.
-
6.
Aftab A, Raina OK, Maxton A, Masih SA, 2024. Advances in diagnostic approaches to Fasciola infection in animals and humans: An overview. J Helminthol 98: e12.
-
7.
Yamasaki H, Aoki T, Oya H, 1989. A cysteine proteinase from the liver fluke Fasciola spp.: purification, characterization, localization and application to immunodiagnosis. Jpn J Parasitol 38: 373–384.
-
8.
Rokni MB, Massoud J, O’Neill SM, Parkinson M, Dalton JP, 2002. Diagnosis of human fasciolosis in the Gilan province of Northern Iran: Application of cathepsin L−ELISA. Diagn Microbiol Infect Dis 44: 175–179.
-
9.
Tantrawatpan C, Maleewong W, Wongkham C, Wongkham S, Intapan PM, Nakashima K, 2005. Serodiagnosis of human fascioliasis by a cystatin capture enzyme−linked immunosorbent assay with recombinant Fasciola gigantica cathepsin L antigen. Am J Trop Med Hyg 72: 82–86.
-
10.
Gonzales Santana B, Dalton JP, Vasquez Camargo F, Parkinson M, Ndao M, 2013. The diagnosis of human fascioliasis by enzyme-linked immunosorbent assay (ELISA) using recombinant cathepsin L protease. PLoS Negl Trop Dis 7: e2414.
-
11.
Meshgi B, Jalousian F, Fathi S, Jahani Z, 2018. Design and synthesis of a new peptide derived from Fasciola gigantica cathepsin L1 with potential application in serodiagnosis of fascioliasis. Exp Parasitol 189: 76–86.
-
12.
Valero MA et al., 2012. Field evaluation of a coproantigen detection test for fascioliasis diagnosis and surveillance in human hyperendemic areas of Andean countries. PLoS Negl Trop Dis 6: e1812.
-
13.
Anuracpreeda P, Chawengkirttikul R, Sobhon P, 2016. Immunodiagnosis of Fasciola gigantica infection using monoclonal antibody-based sandwich ELISA and immunochromatographic assay for detection of circulating cathepsin L1 protease. PLoS One 11: e0145650.
-
14.
Martínez-Sernández V, Muiño L, Perteguer MJ, Gárate T, Mezo M, González-Warleta M, Muro A, Correia da Costa JM, Romarís F, Ubeira FM, 2011. Development and evaluation of a new lateral flow immunoassay for serodiagnosis of human fasciolosis. PLoS Negl Trop Dis 5: e1376.
-
15.
Sadaow L, Yamasaki H, Morishima Y, Sanpool O, Rodpai R, Janwan P, Boonroumkaew P, Maleewong W, Intapan PM, 2020. Effectiveness of Fasciola gigantica excretory−secretory and recombinant cathepsin L antigens for rapid diagnosis of human fascioliasis using immunochromatographic devices. Parasitol Res 119: 3691–3698.
-
16.
Maleewong W, Wongkham C, Intapan PM, Pipitgool V, 1999. Fasciola gigantica-specific antigens: Purification by a continuous-elution method and its evaluation for the diagnosis of human fascioliasis. Am J Trop Med Hyg 61: 648–651.
-
17.
Watanabe S, 1965. A revision of genus Fasciola in Japan with particular reference to F. hepatica and F. gigantica. Morishita K, Kamiya Y, Matsubayashi H, eds. Progress of Medical Parasitology in Japan. Tokyo, Japan: Megura Parasitological Museum, 359–381.
-
18.
Sahba GH, Arfaa F, Farahmandian I, Jalali H, 1972. Animal fascioliasis in Khuzestan, southwestern Iran. J Parasitol 58: 712–716.
-
19.
Lowry OH, Rosebrough NJ, Farr A, Randall RJ, 1951. Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–275.
-
20.
Intapan PM, Maleewong W, Wongkham C, Tomanakarn K, Ieamviteevanich K, Pipitgool V, Sukolapong V, 1998. Excretory-secretory antigenic components of adult Fasciola gigantica recognized by infected human sera. Southeast Asian J Trop Med Public Health 29: 579–583.
-
21.
Elkins DB, Haswell-Elkins M, Anderson RM, 1986. The epidemiology and control of intestinal helminths in the Pulicat Lake region of Southern India. I. Study design and pre- and post-treatment observations on Ascaris lumbricoides infection. Trans R Soc Trop Med Hyg 80: 774–792.
-
22.
Boonyasiri A, Cheunsuchon P, Suputtamongkol Y, Yamasaki H, Sanpool O, Maleewong W, Intapan PM, 2014. Nine human sparganosis cases in Thailand with molecular identification of causative parasite species. Am J Trop Med Hyg 91: 389–393.
-
23.
Janwan P et al., 2016. Development and usefulness of an immunochromatographic device to detect antibodies for rapid diagnosis of human gnathostomiasis. Parasit Vectors 9: 14.
-
24.
Morakote N, Sukhavat K, Khamboonruang C, Siriprasert V, Suphawitayanukul S, Thamasonthi W, 1992. Persistence of IgG, IgM, and IgE antibodies in human trichinosis. Trop Med Parasitol 43: 167–169.
-
25.
Maleewong W, Sombatsawat P, Intapan PM, Wongkham C, Chotmongkol V, 2001. Immunoblot evaluation of the specificity of the 29-kDa antigen from young adult female worms Angiostrongylus cantonensis for immunodiagnosis of human angiostrongyliasis. Asian Pac J Allergy Immunol 19: 267–273.
-
26.
Wongkham C, Maleewong W, Intapan P, Morakote N, Chaicumpa W, 1994. Partially purified antigens of Paragonimus heterotremus for serodiagnosis of human paragonimiasis. Southeast Asian J Trop Med Public Health 25: 176–180.
-
27.
Koga K, Kasuya S, Khamboonruang C, Sukhavat K, Ieda M, Takatsuka N, Kita K, Ohtomo H, 1991. A modified agar plate method for detection of Strongyloides stercoralis. Am J Trop Med Hyg 45: 518–521.
-
28.
Intapan PM, Khotsri P, Kanpittaya J, Chotmongkol V, Maleewong W, Morakote N, 2008. Evaluation of IgG4 and total IgG antibodies against cysticerci and peptide antigens for the diagnosis of human neurocysticercosis by ELISA. Asian Pac J Allergy Immunol 26: 237–244.
-
29.
Katz N, Chaves A, Pellegrino J, 1972. A simple device for quantitative stool thick-smear technique in schistosomiasis mansoni. Rev Inst Med Trop Sao Paulo 14: 397–400.
-
30.
Beaver PC, Jung RC, Cupp EW, 1984. Examination of specimens for parasites. Beaver PC, Jung RC, Cupp EW, eds. Clinical Parasitology. Philadelphia, PA: Lea & Febiger, 733–758.
-
31.
Janwan P, Intapan PM, Sadaow L, Rodpai R, Yamasaki H, Boonroumkaew P, Sanpool O, Thanchomnang T, Sadee P, Maleewong W, 2021. Development of immunochromatographic test kit for rapid detection of specific IgG4 antibody in whole-blood samples for diagnosis of human gnathostomiasis. Diagnostics (Basel) 11: 862.
-
32.
Mangano VD, Modiano D, 2014. Host genetics and parasitic infections. Clin Microbiol Infect 20: 1265–1275.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 1267 | 1267 | 233 |
| Full Text Views | 36 | 36 | 12 |
| PDF Downloads | 44 | 44 | 20 |
Lateral-Flow Antibody Detection Device for the Diagnosis of Human Fascioliasis Using Simulated Whole-Blood Samples
Patcharaporn Boonroumkaew
Patcharaporn Boonroumkaew
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;
Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;
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Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;
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Lakkhana Sadaow
Lakkhana Sadaow
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;
Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;
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Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;
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Nongnapas Kanchanangkul
Nongnapas Kanchanangkul
Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;
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Rutchanee Rodpai
Rutchanee Rodpai
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;
Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;
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Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;
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Oranuch Sanpool
Oranuch Sanpool
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;
Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;
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Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;
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Hiroshi Yamasaki
Hiroshi Yamasaki
Department of Parasitology, National Institute of Infectious Diseases, Tokyo, Japan;
Department of Tropical Medicine and Parasitology, School of Medicine, Juntendo University, Tokyo, Japan
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Department of Tropical Medicine and Parasitology, School of Medicine, Juntendo University, Tokyo, Japan
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Pewpan M. Intapan
Pewpan M. Intapan
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;
Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;
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Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;
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Wanchai Maleewong
wanch_ma@kku.ac.th
Wanchai Maleewong
Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand;
Mekong Health Science Research Institute, Khon Kaen University, Khon Kaen, Thailand;
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ABSTRACT.
Fascioliasis, which is caused by Fasciola hepatica and Fasciola gigantica (F. gigantica), is a public health problem worldwide. Stages infective to humans occur on aquatic plants and in water. The gold standard for diagnosing human infection depends on finding fluke eggs in stool samples. However, this method has low diagnostic sensitivity; eggs from other trematode species might be misidentified as those of Fasciola, and eggs are not voided in ectopic infections. Therefore, serological analysis can support the diagnosis of human fascioliasis. We evaluated whether a new fascioliasis immunochromatographic test kit, which detects specific IgG antibodies using F. gigantica excretory–secretory antigen, can be used for rapid diagnosis from whole blood. The kit (“the fascioliasis whole-blood test kit”) was evaluated under laboratory conditions using 250 whole-blood samples (WBSs), of which 41 were from fascioliasis cases. Results based on simulated WBSs and the corresponding serum samples were compared and showed almost perfect agreement (percent agreement = 97.2%; kappa value = 0.9145). The diagnostic sensitivity and specificity of the test using simulated WBSs were 97.6% and 96.2%, respectively. Comparable values when serum samples were tested were 100.0% and 93.3%, respectively. This kit represents a significant advance because it does not require extensive training of personnel, is easy to use, and can support diagnosis at the bedside or in local and remote hospitals with limited facilities. The kit may also contribute significantly to epidemiological surveys.
- Supplemental Materials (PDF 336.09 KB)
Author Notes
Financial support: This project was funded by grants from the
Disclosure: The ethical guidelines and principles of the Declaration of Helsinki guided all study procedures. The Ethics Committee for Human Research at Khon Kaen University (HE664044) and the Medical Ethics Committee of the National Institute of Infectious Diseases in Tokyo, Japan (No. 177) approved the use of human sera. All data were fully anonymized, and the Human Ethics Committee waived the requirement for informed consent.
Current contact information: Patcharaporn Boonroumkaew, Lakkhana Sadaow, Nongnapas Kanchanangkul, Rutchanee Rodpai, Oranuch Sanpool, Pewpan M. Intapan, and Wanchai Maleewong, Department of Parasitology, Faculty of Medicine, Khon Kaen University, Khon Kaen, Thailand, E-mails: hamooploy@gmail.com, sadaow1986@gmail.com, nongnapas.kanch@gmail.com, rutchanee5020@gmail.com, oransa@kku.ac.th, pewpan@kku.ac.th, and wanch_ma@kku.ac.th. Hiroshi Yamasaki, Department of Parasitology, National Institute of Infectious Diseases, Tokyo, Japan, E-mails: hyamasak@niid.go.jp.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Mas-Coma S, Valero MA, Bargues MD, 2022. Human and animal fascioliasis: Origins and worldwide evolving scenario. Clin Microbiol Rev 35: e0008819.
-
2.
Bui TD, Doanh PN, Saegerman C, Losson B, 2016. Current status of fasciolosis in Vietnam: An update and perspectives. J Helminthol 90: 511–522.
-
3.
Mas-Coma S, 2004. Human fascioliasis. Cotruvo JA, Dufour A, Rees G, eds. Waterborne Zoonoses: Identification, Causes, and Control. London, United Kingdom: World Health Organization, International Water Association Publishing, 305–322.
-
4.
Mas-Coma S, Adela Valero M, Dolores Bargues M, 2015. Fasciola and fasciolopsis. Xiao L, Ryan U, Feng Y, eds. Biology of Food Borne Parasites. 1st ed. New York, NY: Taylor and Francis Group, 371–404.
-
5.
Cwiklinski K, O’Neill SM, Donnelly S, Dalton JP, 2016. A prospective view of animal and human fasciolosis. Parasite Immunol 38: 558–568.
-
6.
Aftab A, Raina OK, Maxton A, Masih SA, 2024. Advances in diagnostic approaches to Fasciola infection in animals and humans: An overview. J Helminthol 98: e12.
-
7.
Yamasaki H, Aoki T, Oya H, 1989. A cysteine proteinase from the liver fluke Fasciola spp.: purification, characterization, localization and application to immunodiagnosis. Jpn J Parasitol 38: 373–384.
-
8.
Rokni MB, Massoud J, O’Neill SM, Parkinson M, Dalton JP, 2002. Diagnosis of human fasciolosis in the Gilan province of Northern Iran: Application of cathepsin L−ELISA. Diagn Microbiol Infect Dis 44: 175–179.
-
9.
Tantrawatpan C, Maleewong W, Wongkham C, Wongkham S, Intapan PM, Nakashima K, 2005. Serodiagnosis of human fascioliasis by a cystatin capture enzyme−linked immunosorbent assay with recombinant Fasciola gigantica cathepsin L antigen. Am J Trop Med Hyg 72: 82–86.
-
10.
Gonzales Santana B, Dalton JP, Vasquez Camargo F, Parkinson M, Ndao M, 2013. The diagnosis of human fascioliasis by enzyme-linked immunosorbent assay (ELISA) using recombinant cathepsin L protease. PLoS Negl Trop Dis 7: e2414.
-
11.
Meshgi B, Jalousian F, Fathi S, Jahani Z, 2018. Design and synthesis of a new peptide derived from Fasciola gigantica cathepsin L1 with potential application in serodiagnosis of fascioliasis. Exp Parasitol 189: 76–86.
-
12.
Valero MA et al., 2012. Field evaluation of a coproantigen detection test for fascioliasis diagnosis and surveillance in human hyperendemic areas of Andean countries. PLoS Negl Trop Dis 6: e1812.
-
13.
Anuracpreeda P, Chawengkirttikul R, Sobhon P, 2016. Immunodiagnosis of Fasciola gigantica infection using monoclonal antibody-based sandwich ELISA and immunochromatographic assay for detection of circulating cathepsin L1 protease. PLoS One 11: e0145650.
-
14.
Martínez-Sernández V, Muiño L, Perteguer MJ, Gárate T, Mezo M, González-Warleta M, Muro A, Correia da Costa JM, Romarís F, Ubeira FM, 2011. Development and evaluation of a new lateral flow immunoassay for serodiagnosis of human fasciolosis. PLoS Negl Trop Dis 5: e1376.
-
15.
Sadaow L, Yamasaki H, Morishima Y, Sanpool O, Rodpai R, Janwan P, Boonroumkaew P, Maleewong W, Intapan PM, 2020. Effectiveness of Fasciola gigantica excretory−secretory and recombinant cathepsin L antigens for rapid diagnosis of human fascioliasis using immunochromatographic devices. Parasitol Res 119: 3691–3698.
-
16.
Maleewong W, Wongkham C, Intapan PM, Pipitgool V, 1999. Fasciola gigantica-specific antigens: Purification by a continuous-elution method and its evaluation for the diagnosis of human fascioliasis. Am J Trop Med Hyg 61: 648–651.
-
17.
Watanabe S, 1965. A revision of genus Fasciola in Japan with particular reference to F. hepatica and F. gigantica. Morishita K, Kamiya Y, Matsubayashi H, eds. Progress of Medical Parasitology in Japan. Tokyo, Japan: Megura Parasitological Museum, 359–381.
-
18.
Sahba GH, Arfaa F, Farahmandian I, Jalali H, 1972. Animal fascioliasis in Khuzestan, southwestern Iran. J Parasitol 58: 712–716.
-
19.
Lowry OH, Rosebrough NJ, Farr A, Randall RJ, 1951. Protein measurement with the Folin phenol reagent. J Biol Chem 193: 265–275.
-
20.
Intapan PM, Maleewong W, Wongkham C, Tomanakarn K, Ieamviteevanich K, Pipitgool V, Sukolapong V, 1998. Excretory-secretory antigenic components of adult Fasciola gigantica recognized by infected human sera. Southeast Asian J Trop Med Public Health 29: 579–583.
-
21.
Elkins DB, Haswell-Elkins M, Anderson RM, 1986. The epidemiology and control of intestinal helminths in the Pulicat Lake region of Southern India. I. Study design and pre- and post-treatment observations on Ascaris lumbricoides infection. Trans R Soc Trop Med Hyg 80: 774–792.
-
22.
Boonyasiri A, Cheunsuchon P, Suputtamongkol Y, Yamasaki H, Sanpool O, Maleewong W, Intapan PM, 2014. Nine human sparganosis cases in Thailand with molecular identification of causative parasite species. Am J Trop Med Hyg 91: 389–393.
-
23.
Janwan P et al., 2016. Development and usefulness of an immunochromatographic device to detect antibodies for rapid diagnosis of human gnathostomiasis. Parasit Vectors 9: 14.
-
24.
Morakote N, Sukhavat K, Khamboonruang C, Siriprasert V, Suphawitayanukul S, Thamasonthi W, 1992. Persistence of IgG, IgM, and IgE antibodies in human trichinosis. Trop Med Parasitol 43: 167–169.
-
25.
Maleewong W, Sombatsawat P, Intapan PM, Wongkham C, Chotmongkol V, 2001. Immunoblot evaluation of the specificity of the 29-kDa antigen from young adult female worms Angiostrongylus cantonensis for immunodiagnosis of human angiostrongyliasis. Asian Pac J Allergy Immunol 19: 267–273.
-
26.
Wongkham C, Maleewong W, Intapan P, Morakote N, Chaicumpa W, 1994. Partially purified antigens of Paragonimus heterotremus for serodiagnosis of human paragonimiasis. Southeast Asian J Trop Med Public Health 25: 176–180.
-
27.
Koga K, Kasuya S, Khamboonruang C, Sukhavat K, Ieda M, Takatsuka N, Kita K, Ohtomo H, 1991. A modified agar plate method for detection of Strongyloides stercoralis. Am J Trop Med Hyg 45: 518–521.
-
28.
Intapan PM, Khotsri P, Kanpittaya J, Chotmongkol V, Maleewong W, Morakote N, 2008. Evaluation of IgG4 and total IgG antibodies against cysticerci and peptide antigens for the diagnosis of human neurocysticercosis by ELISA. Asian Pac J Allergy Immunol 26: 237–244.
-
29.
Katz N, Chaves A, Pellegrino J, 1972. A simple device for quantitative stool thick-smear technique in schistosomiasis mansoni. Rev Inst Med Trop Sao Paulo 14: 397–400.
-
30.
Beaver PC, Jung RC, Cupp EW, 1984. Examination of specimens for parasites. Beaver PC, Jung RC, Cupp EW, eds. Clinical Parasitology. Philadelphia, PA: Lea & Febiger, 733–758.
-
31.
Janwan P, Intapan PM, Sadaow L, Rodpai R, Yamasaki H, Boonroumkaew P, Sanpool O, Thanchomnang T, Sadee P, Maleewong W, 2021. Development of immunochromatographic test kit for rapid detection of specific IgG4 antibody in whole-blood samples for diagnosis of human gnathostomiasis. Diagnostics (Basel) 11: 862.
-
32.
Mangano VD, Modiano D, 2014. Host genetics and parasitic infections. Clin Microbiol Infect 20: 1265–1275.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 1267 | 1267 | 233 |
| Full Text Views | 36 | 36 | 12 |
| PDF Downloads | 44 | 44 | 20 |