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Reference Manager
BibTeX
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-
1.
Mustafa MS, Rasotgi V, Jain S, Gupta V, 2015. Discovery of fifth serotype of dengue virus (DENV-5): a new public health dilemma in dengue control. Med J Armed Forces India 71: 67–70.
-
2.
Guzman MG et al. 2010. Dengue: a continuing global threat. Nat Rev Microbiol 8 (12 Suppl): S7–S16.
-
3.
World Health Organization and Special Programme for Research and Training in Tropical Diseases, 2009. Dengue: Guidelines for Diagnosis, Treatment, Prevention, and Control, new edition. Geneva, Switzerland: WHO and Special Programme for Research and Training in Tropical Diseases.
-
4.
Scott LJ, 2016. Tetravalent dengue vaccine: a review in the prevention of dengue disease. Drugs 76: 1301–1312.
-
5.
Normile D, 2017. Safety concerns derail dengue vaccination program. Science 358: 1514–1515.
-
6.
Acosta EG, Bartenschlager R, 2016. The quest for host targets to combat dengue virus infections. Curr Opin Virol 20: 47–54.
-
7.
Chan KW, Watanabe S, Kavishna R, Alonso S, Vasudevan SG, 2015. Animal models for studying dengue pathogenesis and therapy. Antiviral Res 123: 5–14.
-
8.
Diamond MS, Edgil D, Roberts TG, Lu B, Harris E, 2000. Infection of human cells by dengue virus is modulated by different cell types and viral strains. J Virol 74: 7814–7823.
-
9.
Medina F, Medina JF, Colon C, Vergne E, Santiago GA, Munoz-Jordan JL, 2012. Dengue virus: isolation, propagation, quantification, and storage. Curr Protoc Microbiol 15D: 2.1–2.24.
-
10.
Barr KL, Anderson BD, 2013. Dengue viruses exhibit strain-specific infectivity and entry requirements in vitro. Virus Adapt Treat 5: 1–9.
-
11.
Balsitis SJ, Coloma J, Castro G, Alava A, Flores D, Beatty R, Harris E, 2008. Tropism of replicating dengue virus in mice and humans defined by viral nonstructural protein 3-specific immunohistochemistry. Am J Trop Med Hyg 79: 38.
-
12.
Lindenbach BD, Rice CM, 2007. Flaviviridae: the viruses and their replication. Fields Virol 2007: 1101–1151.
-
13.
Paliwal VK, Garg RK, Juyal R, Husain N, Verma R, Sharma PK, Verma R, Singh MK, 2011. Acute dengue virus myositis: a report of seven patients of varying clinical severity including two cases with severe fulminant myositis. J Neurol Sci 300: 14–18.
-
14.
Salgado DM et al. 2010. Heart and skeletal muscle are targets of dengue virus infection. Pediatr Infect Dis J 29: 238–242.
-
15.
Yacoub S, Wertheim H, Simmons CP, Screaton G, Wills B, 2015. Microvascular and endothelial function for risk prediction in dengue: an observational study. Lancet 385 (Suppl 1): S102.
-
16.
Huang YH, Lei HY, Liu HS, Lin YS, Liu CC, Yeh TM, 2000. Dengue virus infects human endothelial cells and induces IL-6 and IL-8 production. Am J Trop Med Hyg 63: 71–75.
-
17.
Avirutnan P, Malasit P, Seliger B, Bhakdi S, Husmann M, 1998. Dengue virus infection of human endothelial cells leads to chemokine production, complement activation, and apoptosis. J Immunol 161: 6338–6346.
-
18.
Warke RV et al. 2003. Dengue virus induces novel changes in gene expression of human umbilical vein endothelial cells. J Virol 77: 11822–11832.
-
19.
Malavige GN, Fernando S, Fernando DJ, Seneviratne SL, 2004. Dengue viral infections. Postgrad Med J 80: 588–601.
-
20.
Korff T, Kimmina S, Martiny-Baron G, Augustin HG, 2001. Blood vessel maturation in a 3-dimensional spheroidal coculture model: direct contact with smooth muscle cells regulates endothelial cell quiescence and abrogates VEGF responsiveness. FASEB J 15: 447–457.
-
21.
Mora J, Mora R, Lomonte B, Gutiérrez JM, 2008. Effects of bothrops asper snake venom on lymphatic vessels: insights into a hidden aspect of envenomation. PLoS Negl Trop Dis 2: e318.
-
22.
Soto-Garita C, Somogyi T, Vicente-Santos A, Corrales-Aguilar E, 2016. Molecular characterization of two major dengue outbreaks in Costa Rica. Am J Trop Med Hyg 95: 201–205.
-
23.
Morens DM, Halstead SB, Repik PM, Putvatana R, Raybourne N, 1985. Simplified plaque reduction neutralization assay for dengue viruses by semimicro methods in BHK-21 cells: comparison of the BHK suspension test with standard plaque reduction neutralization. J Clin Microbiol 22: 250–254.
-
24.
Gutierrez G, Reines HD, Wulf-Gutierrez ME, 2004. Clinical review: hemorrhagic shock. Crit Care 8: 373–381.
-
25.
Li T, Liu L, Xu J, Yang G, Ming J, 2006. Changes of Rho kinase activity after hemorrhagic shock and its role in shock-induced biphasic response of vascular reactivity and calcium sensitivity. Shock 26: 504–509.
-
26.
Song R, Bian H, Wang X, Huang X, Zhao K-S, 2011. Mitochondrial injury underlies hyporeactivity of arterial smooth muscle in severe shock. Am J Hypertens 24: 45–51.
-
27.
Flint LM, Cryer HM, Simpson CJ, Harris PD, 1984. Microcirculatory norepinephrine constrictor response in hemorrhagic shock. Surgery 96: 240–247.
-
28.
Scully CG et al. 2016. Effect of hemorrhage rate on early hemodynamic responses in conscious sheep. Physiol Rep 4: 1–15.
-
29.
Dorn GW, Becker MW, 1993. Thromboxane A2 stimulated signal transduction in vascular smooth muscle. J Pharmacol Exp Ther 265: 447–456.
-
30.
Preeyasombat C, Treepongkaruna S, Sriphrapradang ACL, 1999. The role of prostacyclin (PGI2) and thromboxane A2 (TXA2) in pathogenesis of dengue hemorrhagic fever (DHF). J Med Assoc Thai 82 (Suppl 1): S16–S21.
| Past two years | Past Year | Past 30 Days | |
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| Abstract Views | 392 | 323 | 26 |
| Full Text Views | 589 | 9 | 0 |
| PDF Downloads | 195 | 8 | 0 |
Dengue Virus Infection of Primary Human Smooth Muscle Cells
Jorge L. Arias-Arias
Jorge L. Arias-Arias
Centro de Investigación en Enfermedades Tropicales (CIET), Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica
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Francisco Vega-Aguilar
Francisco Vega-Aguilar
Centro de Investigación en Enfermedades Tropicales (CIET), Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica
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Eugenia Corrales-Aguilar
Eugenia Corrales-Aguilar
Centro de Investigación en Enfermedades Tropicales (CIET), Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica
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Laya Hun
Laya Hun
Centro de Investigación en Enfermedades Tropicales (CIET), Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica
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Gilbert D. Loría
Gilbert D. Loría
Centro de Investigación en Enfermedades Tropicales (CIET), Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica
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Rodrigo Mora-Rodríguez
Rodrigo Mora-Rodríguez
Centro de Investigación en Enfermedades Tropicales (CIET), Facultad de Microbiología, Universidad de Costa Rica, San José, Costa Rica
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Dengue virus (DENV) infection of humans is presently the most important arthropod-borne viral global threat, for which no suitable or reliable animal model exists. Reports addressing the effect of DENV on vascular components other than endothelial cells are lacking. Dengue virus infection of vascular smooth muscle cells, which play a physiological compensatory response to hypotension in arteries and arterioles, has not been characterized, thus precluding our understanding of the role of these vascular components in dengue pathogenesis. Therefore, we studied the permissiveness of primary human umbilical artery smooth muscle cells (HUASMC) to DENV 1–4 infection and compared with the infection in the previously reported primary human umbilical vein endothelial cells (HUVEC) and the classically used, non-transformed, and highly permissive Lilly Laboratories Cell-Monkey Kidney 2 cells. Our results show that HUASMC are susceptible and productive to infection with the four DENV serotypes, although to a lesser extent when compared with the other cell lines. This is the first report of DENV permissiveness in human smooth muscle cells, which might represent an unexplored pathophysiological contributor to the vascular collapse observed in severe human dengue infection.
Author Notes
Financial support: This work was supported by Universidad de Costa Rica (project VI-803-A5-025), Consejo Nacional para Investigaciones Científicas y Tecnológicas (project FI-182-10), and the Florida Ice and Farm Co.
Authors’ addresses: Jorge L. Arias-Arias, Francisco Vega-Aguilar, Eugenia Corrales-Aguilar, Laya Hun, Gilbert D. Loría, and Rodrigo Mora-Rodríguez, Facultad de Microbiología, Centro de Investigación en Enfermedades Tropicales, Universidad de Costa Rica, Ciudad Universitaria Rodrigo Facio, San José, Costa Rica, E-mails: jorgeluis.arias@ucr.ac.cr, francisco.vega@ucr.ac.cr, eugenia.corrales@ucr.ac.cr, ruchlia.hun@ucr.ac.cr, gilbert.loria@ucr.ac.cr, and rodrigo.morarodriguez@ucr.ac.cr.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Mustafa MS, Rasotgi V, Jain S, Gupta V, 2015. Discovery of fifth serotype of dengue virus (DENV-5): a new public health dilemma in dengue control. Med J Armed Forces India 71: 67–70.
-
2.
Guzman MG et al. 2010. Dengue: a continuing global threat. Nat Rev Microbiol 8 (12 Suppl): S7–S16.
-
3.
World Health Organization and Special Programme for Research and Training in Tropical Diseases, 2009. Dengue: Guidelines for Diagnosis, Treatment, Prevention, and Control, new edition. Geneva, Switzerland: WHO and Special Programme for Research and Training in Tropical Diseases.
-
4.
Scott LJ, 2016. Tetravalent dengue vaccine: a review in the prevention of dengue disease. Drugs 76: 1301–1312.
-
5.
Normile D, 2017. Safety concerns derail dengue vaccination program. Science 358: 1514–1515.
-
6.
Acosta EG, Bartenschlager R, 2016. The quest for host targets to combat dengue virus infections. Curr Opin Virol 20: 47–54.
-
7.
Chan KW, Watanabe S, Kavishna R, Alonso S, Vasudevan SG, 2015. Animal models for studying dengue pathogenesis and therapy. Antiviral Res 123: 5–14.
-
8.
Diamond MS, Edgil D, Roberts TG, Lu B, Harris E, 2000. Infection of human cells by dengue virus is modulated by different cell types and viral strains. J Virol 74: 7814–7823.
-
9.
Medina F, Medina JF, Colon C, Vergne E, Santiago GA, Munoz-Jordan JL, 2012. Dengue virus: isolation, propagation, quantification, and storage. Curr Protoc Microbiol 15D: 2.1–2.24.
-
10.
Barr KL, Anderson BD, 2013. Dengue viruses exhibit strain-specific infectivity and entry requirements in vitro. Virus Adapt Treat 5: 1–9.
-
11.
Balsitis SJ, Coloma J, Castro G, Alava A, Flores D, Beatty R, Harris E, 2008. Tropism of replicating dengue virus in mice and humans defined by viral nonstructural protein 3-specific immunohistochemistry. Am J Trop Med Hyg 79: 38.
-
12.
Lindenbach BD, Rice CM, 2007. Flaviviridae: the viruses and their replication. Fields Virol 2007: 1101–1151.
-
13.
Paliwal VK, Garg RK, Juyal R, Husain N, Verma R, Sharma PK, Verma R, Singh MK, 2011. Acute dengue virus myositis: a report of seven patients of varying clinical severity including two cases with severe fulminant myositis. J Neurol Sci 300: 14–18.
-
14.
Salgado DM et al. 2010. Heart and skeletal muscle are targets of dengue virus infection. Pediatr Infect Dis J 29: 238–242.
-
15.
Yacoub S, Wertheim H, Simmons CP, Screaton G, Wills B, 2015. Microvascular and endothelial function for risk prediction in dengue: an observational study. Lancet 385 (Suppl 1): S102.
-
16.
Huang YH, Lei HY, Liu HS, Lin YS, Liu CC, Yeh TM, 2000. Dengue virus infects human endothelial cells and induces IL-6 and IL-8 production. Am J Trop Med Hyg 63: 71–75.
-
17.
Avirutnan P, Malasit P, Seliger B, Bhakdi S, Husmann M, 1998. Dengue virus infection of human endothelial cells leads to chemokine production, complement activation, and apoptosis. J Immunol 161: 6338–6346.
-
18.
Warke RV et al. 2003. Dengue virus induces novel changes in gene expression of human umbilical vein endothelial cells. J Virol 77: 11822–11832.
-
19.
Malavige GN, Fernando S, Fernando DJ, Seneviratne SL, 2004. Dengue viral infections. Postgrad Med J 80: 588–601.
-
20.
Korff T, Kimmina S, Martiny-Baron G, Augustin HG, 2001. Blood vessel maturation in a 3-dimensional spheroidal coculture model: direct contact with smooth muscle cells regulates endothelial cell quiescence and abrogates VEGF responsiveness. FASEB J 15: 447–457.
-
21.
Mora J, Mora R, Lomonte B, Gutiérrez JM, 2008. Effects of bothrops asper snake venom on lymphatic vessels: insights into a hidden aspect of envenomation. PLoS Negl Trop Dis 2: e318.
-
22.
Soto-Garita C, Somogyi T, Vicente-Santos A, Corrales-Aguilar E, 2016. Molecular characterization of two major dengue outbreaks in Costa Rica. Am J Trop Med Hyg 95: 201–205.
-
23.
Morens DM, Halstead SB, Repik PM, Putvatana R, Raybourne N, 1985. Simplified plaque reduction neutralization assay for dengue viruses by semimicro methods in BHK-21 cells: comparison of the BHK suspension test with standard plaque reduction neutralization. J Clin Microbiol 22: 250–254.
-
24.
Gutierrez G, Reines HD, Wulf-Gutierrez ME, 2004. Clinical review: hemorrhagic shock. Crit Care 8: 373–381.
-
25.
Li T, Liu L, Xu J, Yang G, Ming J, 2006. Changes of Rho kinase activity after hemorrhagic shock and its role in shock-induced biphasic response of vascular reactivity and calcium sensitivity. Shock 26: 504–509.
-
26.
Song R, Bian H, Wang X, Huang X, Zhao K-S, 2011. Mitochondrial injury underlies hyporeactivity of arterial smooth muscle in severe shock. Am J Hypertens 24: 45–51.
-
27.
Flint LM, Cryer HM, Simpson CJ, Harris PD, 1984. Microcirculatory norepinephrine constrictor response in hemorrhagic shock. Surgery 96: 240–247.
-
28.
Scully CG et al. 2016. Effect of hemorrhage rate on early hemodynamic responses in conscious sheep. Physiol Rep 4: 1–15.
-
29.
Dorn GW, Becker MW, 1993. Thromboxane A2 stimulated signal transduction in vascular smooth muscle. J Pharmacol Exp Ther 265: 447–456.
-
30.
Preeyasombat C, Treepongkaruna S, Sriphrapradang ACL, 1999. The role of prostacyclin (PGI2) and thromboxane A2 (TXA2) in pathogenesis of dengue hemorrhagic fever (DHF). J Med Assoc Thai 82 (Suppl 1): S16–S21.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 392 | 323 | 26 |
| Full Text Views | 589 | 9 | 0 |
| PDF Downloads | 195 | 8 | 0 |