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1.
Prasad N, Murdoch DR, Reyburn H, Crump JA, 2015. Etiology of severe febrile illness in low- and middle-income countries: a systematic review. PLoS One 10: e0127962.
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2.
Iroh Tam PY, Obaro SK, Storch G, 2016. Challenges in the etiology and diagnosis of acute febrile illness in children in low- and middle-income countries. J Pediatric Infect Dis Soc 5: 190–205.
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3.
Balajee SA, Arthur R, Mounts AW, 2016. Global health security: building capacities for early event detection, epidemiologic workforce, and laboratory response. Health Secur 14: 424–432.
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4.
Liu J et al. 2016. Development of a taqman array card for acute-febrile-illness outbreak investigation and surveillance of emerging pathogens, including ebola virus. J Clin Microbiol 54: 49–58.
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5.
Liu J et al. 2014. Development and assessment of molecular diagnostic tests for 15 enteropathogens causing childhood diarrhoea: a multicentre study. Lancet Infect Dis 14: 716–724.
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6.
Diaz MH et al. 2013. Optimization of multiple pathogen detection using the taqman array card: application for a population-based study of neonatal infection. PLoS One 8: e66183.
-
7.
Nga TV et al. 2010. The sensitivity of real-time PCR amplification targeting invasive Salmonella serovars in biological specimens. BMC Infect Dis 10: 125.
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8.
Liu J et al. 2016. Use of quantitative molecular diagnostic methods to identify causes of diarrhoea in children: a reanalysis of the gems case-control study. Lancet 388: 1291–1301.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 509 | 399 | 35 |
| Full Text Views | 640 | 14 | 1 |
| PDF Downloads | 212 | 9 | 1 |
Use of TaqMan Array Cards to Screen Outbreak Specimens for Causes of Febrile Illness in Tanzania
Ahmed Abade
Ahmed Abade
Field Epidemiology and Laboratory Training Program, Dar es Salaam, Tanzania;
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Rachel B. Eidex
Rachel B. Eidex
Center for Disease Control and Prevention, Atlanta, Georgia and Dar es Salaam, Tanzania;
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Athanasia Maro
Athanasia Maro
Kilimanjaro Clinical Research Institute, Moshi, Tanzania;
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Jean Gratz
Jean Gratz
University of Virginia, Charlottesville, Virginia;
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Jie Liu
Jie Liu
University of Virginia, Charlottesville, Virginia;
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Ireen Kiwelu
Ireen Kiwelu
Kilimanjaro Clinical Research Institute, Moshi, Tanzania;
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Buliga Mujaga
Buliga Mujaga
Kilimanjaro Clinical Research Institute, Moshi, Tanzania;
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Maria E. Kelly
Maria E. Kelly
National Health Laboratory Quality Assurance and Training Center (NHLQATC), Dar es Salaam, Tanzania
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Blandina T. Mmbaga
Blandina T. Mmbaga
Kilimanjaro Clinical Research Institute, Moshi, Tanzania;
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James J. Gibson
James J. Gibson
Center for Disease Control and Prevention, Atlanta, Georgia and Dar es Salaam, Tanzania;
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Fausta Mosha
Fausta Mosha
National Health Laboratory Quality Assurance and Training Center (NHLQATC), Dar es Salaam, Tanzania
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Eric R. Houpt
Eric R. Houpt
University of Virginia, Charlottesville, Virginia;
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We describe the deployment of a custom-designed molecular diagnostic TaqMan Array Card (TAC) to screen for 31 bacterial, protozoal, and viral etiologies in blood from outbreaks of acute febrile illness in Tanzania during 2015–2017. On outbreaks notified to the Tanzanian Ministry of Health, epidemiologists were dispatched and specimens were collected, transported to a central national laboratory, and tested by TAC within 2 days. This algorithm streamlined investigation, diagnosed a typhoid outbreak, and excluded dozens of other etiologies. This method is usable in-country and may be incorporated into algorithms for diagnosing outbreaks.
Author Notes
Financial support: This work was supported by the CDC Global Health Security Partner Engagement Cooperative Agreement #U2GGH001688.
Authors’ addresses: Ahmed Abade, Field Epidemiology and Laboratory Training Program, Dar es Salaam, Tanzania, E-mail: ahmedabade@yahoo.com. Rachel B. Eidex and James J. Gibson, Center for Disease Control and Prevention, Atlanta, GA and Dar es Salaam, Tanzania, E-mails: zvd3@cdc.gov and jerry.gibson.sc@gmail.com. Athanasia Maro, Ireen Kiwelu, Buliga Mujaga, and Blandina T. Mmbaga, Kilimanjaro Clinical Research Institute, Moshi, Tanzania, E-mails: athanasia.maro@gmail.com, i.kiwelu@kcri.ac.tz, b.mujaga@gmail.com, and b.mmbaga@kcri.ac.tz. Jean Gratz, Jie Liu, and Eric R. Houpt, University of Virginia, Charlottesville, VA, E-mails: jean.gratz@gmail.com, jl5yj@virginia.edu, and erh6k@virginia.edu. Maria Kelly and Fausta Mosha, National Health Laboratory Quality Assurance and Training Center, Dar es Salaam, Tanzania, E-mails: mariadorcas8@gmail.com and fausta_mosha@yahoo.com.
ProCite
RefWorks
Reference Manager
BibTeX
Zotero
EndNote
-
1.
Prasad N, Murdoch DR, Reyburn H, Crump JA, 2015. Etiology of severe febrile illness in low- and middle-income countries: a systematic review. PLoS One 10: e0127962.
-
2.
Iroh Tam PY, Obaro SK, Storch G, 2016. Challenges in the etiology and diagnosis of acute febrile illness in children in low- and middle-income countries. J Pediatric Infect Dis Soc 5: 190–205.
-
3.
Balajee SA, Arthur R, Mounts AW, 2016. Global health security: building capacities for early event detection, epidemiologic workforce, and laboratory response. Health Secur 14: 424–432.
-
4.
Liu J et al. 2016. Development of a taqman array card for acute-febrile-illness outbreak investigation and surveillance of emerging pathogens, including ebola virus. J Clin Microbiol 54: 49–58.
-
5.
Liu J et al. 2014. Development and assessment of molecular diagnostic tests for 15 enteropathogens causing childhood diarrhoea: a multicentre study. Lancet Infect Dis 14: 716–724.
-
6.
Diaz MH et al. 2013. Optimization of multiple pathogen detection using the taqman array card: application for a population-based study of neonatal infection. PLoS One 8: e66183.
-
7.
Nga TV et al. 2010. The sensitivity of real-time PCR amplification targeting invasive Salmonella serovars in biological specimens. BMC Infect Dis 10: 125.
-
8.
Liu J et al. 2016. Use of quantitative molecular diagnostic methods to identify causes of diarrhoea in children: a reanalysis of the gems case-control study. Lancet 388: 1291–1301.
| Past two years | Past Year | Past 30 Days | |
|---|---|---|---|
| Abstract Views | 509 | 399 | 35 |
| Full Text Views | 640 | 14 | 1 |
| PDF Downloads | 212 | 9 | 1 |