1921
Volume 96, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645

Abstract

Abstract

The zoonotic, vector-borne parasite causes Chagas disease throughout the Americas, but human and veterinary health burdens in the United States are unknown. We conducted a cross-sectional prevalence study in indigent, medically underserved human and cohabiting canine populations of seven south Texas border communities, known as colonias. Defining positivity as those samples that were positive on two or more independent tests, we found 1.3% seroprevalence in 233 humans, including one child born in the United States with only short-duration travel to Mexico. Additionally, a single child with no travel outside south Texas was positive on only a single test. Among 209 dogs, seroprevalence was 19.6%, but adjusted to 31.6% when including those dogs positive on only one test and extrapolating potential false negatives. Parasite DNA was detected in five dogs, indicating potential parasitemia. Seropositive dogs lived in all sampled colonias with no difference in odds of positivity across age, sex, or breed. Colonia residents collected two adult and one nymph triatomine from around their homes; one of three bugs was infected with , and blood meal hosts were molecularly determined to include dog, human, and raccoon. Dogs and the infected vector all harbored discrete typing unit I, which has previously been implicated in human disease in the United States. Colonias harbor active transmission cycles and should be a priority in outreach and vector control initiatives.

[open-access] This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

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2017-04-05
2017-10-18
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References

  1. World Health Organization, 2015. Chagas disease in Latin America: an epidemiological update based on 2010 estimates. Wkly Epidemiol Rec 6: 3334.
  2. Bern C, Montgomery SP, , 2009. An estimate of the burden of Chagas disease in the United States. Clin Infect Dis 49: e52e54.[Crossref]
  3. Schmunis GA, , 2007. Epidemiology of Chagas disease in non-endemic countries: the role of international migration. Mem Inst Oswaldo Cruz 102 (Suppl): 7585.[Crossref]
  4. Hanford EJ, Zhan FB, Lu Y, Giordano A, , 2007. Chagas disease in Texas: recognizing the significance and implications of evidence in the literature. Soc Sci Med 65: 6079.[Crossref]
  5. Garcia MN, Aguilar D, Gorchakov R, Rossmann SN, Montgomery SP, Rivera H, Woc-Colburn L, Hotez PJ, Murray KO, , 2015. Case report: evidence of autochthonous Chagas disease in southeastern Texas. Am J Trop Med Hyg 92: 325330.[Crossref]
  6. Cantey PT, Stramer SL, Townsend RL, Kamel H, Ofafa K, Todd CW, Currier M, Hand S, Varnado W, Dotson E, Hall C, Jett PL, Montgomery SP, , 2012. The United States Trypanosoma cruzi infection study: evidence for vector-borne transmission of the parasite that causes Chagas disease among United States blood donors. Transfusion 52: 19221930.[Crossref]
  7. Curtis-Robles R, Wozniak EJ, Auckland LD, Hamer GL, Hamer SA, , 2015. Combining public health education and disease ecology research: using citizen science to assess Chagas disease entomological risk in Texas. PLoS Negl Trop Dis 9: 112.[Crossref]
  8. Bern C, Kjos S, Yabsley MJ, Montgomery SP, , 2011. Trypanosoma cruzi and Chagas' disease in the United States. Clin Microbiol Rev 24: 655681.[Crossref]
  9. Barr SC, Gossett KA, Klei TR, , 1991. Clinical, clinicopathologic, and parasitologic observations of trypanosomiasis in dogs infected with North American Trypanosoma cruzi isolates. Am J Vet Res 52: 954960.
  10. Kjos S, Snowden K, Craig T, Lewis B, Ronald N, Olson J, , 2008. Distribution and characterization of canine Chagas disease in Texas. Vet Parasitol 152: 249256.[Crossref]
  11. Tenney TD, Curtis-Robles R, Snowden KF, Hamer SA, , 2014. Shelter dogs as sentinels for Trypanosoma cruzi transmission across Texas. Emerg Infect Dis 20: 13231326.[Crossref]
  12. Beard CB, Pye G, Steurer FJ, Rodriguez R, Campman R, Peterson AT, Ramsey J, Wirtz RA, Robinson LE, , 2003. Chagas disease in a domestic transmission cycle, southern Texas, USA. Emerg Infect Dis 9: 103105.[Crossref]
  13. Nieto PD, Boughton R, Dorn PL, Steurer F, Raychaudhuri S, Estandiari J, Goncalves E, Diaz J, Malone JB, , 2009. Comparison of two immunochromatographic assays and the indirect immunofluorescence antibody test for diagnosis of Trypanosoma cruzi infection in dogs in south central Louisiana. Vet Parasitol 165: 241247.[Crossref]
  14. Barr SC, Van Beek O, Carlisle-Nowak MS, Lopez JW, Kirchoff LV, Allison N, Zajac A, de Lahunta A, Schlafer DH, Crandall WT, , 1995. Trypanosoma cruzi infection in Walker hounds from Virginia. Am J Vet Res 56: 10371044.
  15. Gürtler RE, Cardinal MV, , 2015. Reservoir host competence and the role of domestic and commensal hosts in the transmission of Trypanosoma cruzi . Acta Trop 151: 3250.[Crossref]
  16. Bustamante DM, De Urioste-Stone SM, Juárez JG, Pennington PM, , 2014. Ecological, social and biological risk factors for continued Trypanosoma cruzi transmission by Triatoma dimidiata in Guatemala. PLoS One 9: 343352.
  17. Gürtler RE, Cecere MC, Rubel DN, Schweigmann NJ, , 1992. Determinants of the domiciliary density of Triatoma infestans, vector of Chagas disease. Med Vet Entomol 6: 7583.[Crossref]
  18. Dumonteil E, Nouvellet P, Rosecrans K, Ramirez-Sierra MJ, Gamboa-León R, Cruz-Chan V, Rosado-Vallado M, Gourbière S, , 2013. Eco-bio-social determinants for house infestation by non-domiciliated Triatoma dimidiata in the Yucatan Peninsula, Mexico. PLoS Negl Trop Dis 7: 19.
  19. Gürtler R, Cécere M, Rubel D, Petersen R, Schweigmann N, Lauricella M, Bujas M, , 1991. Chagas disease in north-west Argentina: infected dogs as a risk factor for the domestic transmission of Trypanosoma cruzi . Trans R Soc Trop Med Hyg 85: 741745.[Crossref]
  20. Mott KE, Mota EA, Sherlock I, Hoff R, Muniz TM, Oliveira TS, Draper CC, Lehman JS, Morrow RH, de Oliveira TS, , 1978. Trypanosoma cruzi infection in dogs and cats and household seroreactivity to T. cruzi in a rural community in northeast Brazil. Am J Trop Med Hyg 27: 11231127.
  21. Castañera MB, Lauricella MA, Chuit R, Gürtler RE, , 1998. Evaluation of dogs as sentinels of the transmission of Trypanosoma cruzi in a rural area of north-western Argentina. Ann Trop Med Parasitol 92: 671683.[Crossref]
  22. Castillo-Neyra R, Chou Chu L, Quispe-Machaca V, Ancca-Juarez J, Malaga Chavez FS, Bastos Mazuelos M, Naquira C, Bern C, Gilman RH, Levy MZ, , 2015. The potential of canine sentinels for reemerging Trypanosoma cruzi transmission. Prev Vet Med 120: 349356.[Crossref]
  23. Housing Assistance Council, 2013. Housing in the Border Colonias. Washington, DC: Housing Assistance Council, 111.
  24. Barton J, Perimeter ER, Marquez RR, , 2015. Las Colonias in the 21st Century: Progress along the Texas-Mexico Border. Dallas, TX: Federal Reserve Bank of Dallas, 133.
  25. Núñez-Mchiri GG, , 2009. The political ecology of the colonias on the U.S.-Mexico border: human-environmental challenges and community responses in southern New Mexico. South Rural Sociol 24: 6791.
  26. Rocha FL, Roque ALR, de Lima JS, Cheida CC, Lemos FG, de Azevedo FC, Arrais RC, Bilac D, Herrera HM, Mourão G, Jansen AM, , 2013. Trypanosoma cruzi infection in neotropical wild carnivores (Mammalia: Carnivora): at the top of the T. cruzi transmission chain. PLoS One 8: e67463.[Crossref]
  27. Thomas ME, Rasweiler JJ, IV D'Alessandro A, , 2007. Experimental transmission of the parasitic flagellates Trypanosoma cruzi and Trypanosoma rangeli between triatomine bugs or mice and captive neotropical bats. Mem Inst Oswaldo Cruz 102: 559565.[Crossref]
  28. Curtis-Robles R, Lewis BC, Hamer SA, , 2016. High Trypanosoma cruzi infection prevalence associated with minimal cardiac pathology among wild carnivores in central Texas. Int J Parasitol Parasites Wildl 5: 117123.[Crossref]
  29. Burkholder JE, Allison TC, Kelly VP, , 1980. Trypanosoma cruzi (Chagas) (Protozoa: Kinetoplastida) in invertebrate, reservoir, and human hosts of the Lower Rio Grande Valley of Texas. J Parasitol 66: 305311.[Crossref]
  30. Luquetti AO, Ponce C, Ponce E, Esfandiari J, Schijman A, Revollo S, Añez N, Zingales B, Ramgel-Aldao R, Gonzalez A, Levin MJ, Umezawa ES, Franco da Silveira J, , 2003. Chagas' disease diagnosis: a multicentric evaluation of Chagas Stat-Pak, a rapid immunochromatographic assay with recombinant proteins of Trypanosoma cruzi . Diagn Microbiol Infect Dis 46: 265271.[Crossref]
  31. Ponce C, Ponce E, Vinelli E, Montoya A, De Aguilar V, Gonzalez A, Zingales B, Rangel-Aldao R, Levin MJ, Esfandiari J, Umezawa ES, Luquetti AO, da Silveira JF, , 2005. Validation of a rapid and reliable test for diagnosis of Chagas' disease by detection of Trypanosoma cruzi-specific antibodies in blood of donors and patients in Central America. J Clin Microbiol 43: 50655068.[Crossref]
  32. Sánchez-Camargo CL, Albajar-Viñas P, Wilkins PP, Nieto J, Leiby DA, Paris L, Scollo K, Flórez C, Guzmán-Bracho C, Luquetti AO, Calvo N, Tadokoro K, Saez-Alquezar A, Palma PP, Martin M, Flevaud L, , 2014. Comparative evaluation of 11 commercialized rapid diagnostic tests for detecting Trypanosoma cruzi antibodies in serum banks in areas of endemicity and nonendemicity. J Clin Microbiol 52: 25062512.[Crossref]
  33. Chappuis F, Mauris A, Holst M, Albajar-Vinas P, Jannin J, Luquetti AO, Jackson Y, , 2010. Validation of a rapid immunochromatographic assay for diagnosis of Trypanosoma cruzi infection among Latin-American migrants in Geneva, Switzerland. J Clin Microbiol 48: 29482952.[Crossref]
  34. Barfield CA, Barney RS, Crudder CH, Wilmoth JL, Stevens DS, Mora-Garcia S, Yanovsky MJ, Weigl BH, Yanovsky J, , 2011. A highly sensitive rapid diagnostic test for Chagas disease that utilizes a recombinant Trypanosoma cruzi antigen. IEEE Trans Biomed Eng 58: 814817.[Crossref]
  35. Chippaux JP, Santalla JA, Postigo JR, Romero M, Salas Clavijo NA, Schneider D, Brutus L, , 2009. Sensitivity and specificity of Chagas Stat-Pak test in Bolivia. Trop Med Int Health 14: 732735.[Crossref]
  36. Verani JR, Seitz A, Gilman RH, LaFuente C, Galdos-Cardenas G, Kawai V, de LaFuente E, Ferrufino L, Bowman NM, Pinedo-Cancino V, Levy MZ, Steurer F, Todd CW, Kirchhoff LV, Cabrera L, Verastegui M, Bern C, , 2009. Geographic variation in the sensitivity of recombinant antigen-based rapid tests for chronic Trypanosoma cruzi infection. Am J Trop Med Hyg 80: 410415.
  37. Barbabosa-Pliego A, Díaz-Albiter HM, Ochoa-García L, Aparicio-Burgos E, López-Heydeck SM, Velásquez-Ordoñez V, Fajardo-Muñoz RC, Díaz-González S, De Oca-Jimenez RM, Barbosa-Mireles M, Guzmán-Bracho C, Estrada-Franco JG, Garg NJ, Vázquez-Chagoyán JC, , 2009. Trypanosoma cruzi circulating in the southern region of the State of Mexico (Zumpahuacan) are pathogenic: a dog model. Am J Trop Med Hyg 81: 390395.
  38. Houghton RL, Stevens YY, Hjerrild K, Guderian J, Okamoto M, Kabir M, Reed SG, Leiby DA, Morrow WJW, Lorca M, Raychaudhuri S, , 2009. Lateral flow immunoassay for diagnosis of Trypanosoma cruzi infection with high correlation to the radioimmunoprecipitation assay. Clin Vaccine Immunol 16: 515520.[Crossref]
  39. Shah V, Ferrufino L, Gilman RH, Ramirez M, Saenza E, Malaga E, Sanchez G, Okamoto EE, Sherbuck JE, Clark EH, Galdos-Cardenas G, Bozo R, Flores-Franco JL, Colanzi R, Verastegui M, Bern C, , 2014. Field evaluation of the InBios Chagas detect plus rapid test in serum and whole-blood specimens in Bolivia. Clin Vaccine Immunol 21: 16451649.[Crossref]
  40. Duffy T, Cura CI, Ramirez JC, Abate T, Cayo NM, Parrado R, Bello ZD, Velazquez E, Muñoz-Calderon A, Juiz NA, Basile J, Garcia L, Riarte A, Nasser JR, Ocampo SB, Yadon ZE, Torrico F, de Noya BA, Ribeiro I, Schijman AG, , 2013. Analytical performance of a multiplex real-time PCR assay using TaqMan probes for quantification of Trypanosoma cruzi satellite DNA in blood samples. PLoS Negl Trop Dis 7: e2000.[Crossref]
  41. Piron M, Fisa R, Casamitjana N, López-Chejade P, Puig L, Vergés M, Gascón J, Gómez i Prat J, Portús M, Sauleda S, , 2007. Development of a real-time PCR assay for Trypanosoma cruzi detection in blood samples. Acta Trop 103: 195200.[Crossref]
  42. Cura CI, Duffy T, Lucero RH, Bisio M, Péneau J, Jimenez-Coello M, Calabuig E, Gimenez MJ, Valencia Ayala E, Kjos SA, Santalla J, Mahaney SM, Cayo NM, Nagel C, Barcán L, Málaga Machaca ES, Acosta Viana KY, Brutus L, Ocampo SB, Aznar C, Cuba Cuba CA, Gürtler RE, Ramsey JM, Ribeiro I, VandeBerg JL, Yadon ZE, Osuna A, Schijman AG, , 2015. Multiplex real-time PCR assay using TaqMan probes for the identification of Trypanosoma cruzi DTUs in biological and clinical samples. PLoS Negl Trop Dis 9: e0003765.[Crossref]
  43. Lent H, Wygodzinsky P, , 1979. Revision of the Triatominae (Hemiptera, Reduviidae), and their significance as vectors of Chagas' disease. Bull Am Mus Nat Hist 163: 123520.
  44. Cupp EW, Zhang D, Yue X, Cupp MS, Guyer C, Sprenger TR, Unnasch TR, , 2004. Identification of reptilian and amphibian blood meals from mosquitoes in an eastern equine encephalomyelitis virus focus in central Alabama. Am J Trop Med Hyg 71: 272276.
  45. Hamer GL, Kitron UD, Goldberg TL, Brawn JD, Loss SR, Ruiz MO, Hayes DB, Walker ED, , 2009. Host selection by Culex pipiens mosquitoes and West Nile virus amplification. Am J Trop Med Hyg 80: 268278.
  46. Molaei G, Andreadis TG, Armstrong PM, Anderson JF, Vossbrinck CR, , 2006. Host feeding patterns of Culex mosquitoes and West Nile virus transmission, northeastern United States. Emerg Infect Dis 12: 468474.[Crossref]
  47. Kjos SA, Marcet PL, Yabsley MJ, Kitron U, Snowden KF, Logan KS, Barnes JC, Dotson EM, , 2013. Identification of bloodmeal sources and Trypanosoma cruzi infection in triatomine bugs (Hemiptera: Reduviidae) from residential settings in Texas, the United States. J Med Entomol 50: 11261139.[Crossref]
  48. Boakye D, Tang J, Truc P, Merriweather A, Unnasch T, , 1999. Identification of bloodmeals in haematophagous Diptera by cytochrome B heteroduplex analysis. Med Vet Entomol 13: 282287.[Crossref]
  49. R Development Core Team, 2008. R: A Language and Environment for Statistical Computing. Available at: https://r-project.org. Accessed June 21, 2016.
  50. Gürtler RE, Lauricela M, Solarz ND, Bujas MA, Wisnivesky-Colli C, , 1986. Dynamics of transmission of Trypanosoma cruzi in a rural area of Argentina. I—The dog reservoir: an epidemiological profile. Rev Inst Med Trop Sao Paulo 28: 411.
  51. Cardinal M, Lauricella M, Marcet P, Orozco M, Kitron U, Gürtler R, , 2007. Impact of community-based vector control on house infestation and Trypanosoma cruzi infection in Triatoma infestans, dogs and cats in the Argentine Chaco. Acta Trop 103: 201211.[Crossref]
  52. Woody NC, DeDianous N, Woody HB, , 1961. American trypanosomiasis II. Current serologic studies in Chagas' disease. J Pediatr 58: 738745.[Crossref]
  53. Garcia MN, Woc-Colburn L, Rossmann SN, Townsend RL, Stramer SL, Bravo M, Kamel H, Beddard R, Townsend M, Oldham R, Bottazzi ME, Hotez PJ, Murray KO, , 2016. Trypanosoma cruzi screening in Texas blood donors, 2008–2012. Epidemiol Infect 144: 10101013.[Crossref]
  54. Nouvellet P, Dumonteil E, Gourbière S, , 2013. The improbable transmission of Trypanosoma cruzi to human: the missing link in the dynamics and control of Chagas disease. PLoS Negl Trop Dis 7: e2505.[Crossref]
  55. Gürtler RE, Cecere MC, Lauricella MA, Petersen RM, Chuit R, Segura EL, Cohen JE, , 2005. Incidence of Trypanosoma cruzi infection among children following domestic reinfestation after insecticide spraying in rural northwestern Argentina. Am J Trop Med Hyg 73: 95103.
  56. Minneman RM, Hennink MM, Nicholls A, Salek SS, Palomeque FS, Khawja A, Albor LC, Pennock CC, Leon JS, , 2012. Barriers to testing and treatment for Chagas disease among Latino immigrants in Georgia. J Parasitol Res 2012: 295034.[Crossref]
  57. Umstattd Meyer MR, Sharkey JR, Patterson MS, Dean WR, , 2013. Understanding contextual barriers, supports, and opportunities for physical activity among Mexican-origin children in Texas border colonias: a descriptive study. BMC Public Health 13: 115.[Crossref]
  58. Mier N, Ory MG, Zhan D, Conkling M, Sharkey JR, Burdine JN, , 2008. Health-related quality of life among Mexican Americans living in colonias at the Texas-Mexico border. Soc Sci Med 66: 17601771.[Crossref]
  59. Estrada-Franco JG, Bhatia V, Diaz-Albiter H, Ochoa-Garcia L, Barbabosa A, Vazquez-Chagoyan JC, Martinez-Perez MA, Guzman-Bracho C, Garg N, , 2006. Human Trypanosoma cruzi infection and seropositivity in dogs, Mexico. Emerg Infect Dis 12: 624630.[Crossref]
  60. Enriquez GF, Bua J, Orozco MM, Wirth S, Schijman AG, Gürtler RE, Cardinal MV, , 2014. High levels of Trypanosoma cruzi DNA determined by qPCR and infectiousness to Triatoma infestans support dogs and cats are major sources of parasites for domestic transmission. Infect Genet Evol 25: 3643.[Crossref]
  61. Roellig DM, Brown EL, Barnabé C, Tibayrenc M, Steurer FJ, Yabsley MJ, , 2008. Molecular typing of Trypanosoma cruzi isolates, United States. Emerg Infect Dis 14: 11231125.[Crossref]
  62. American Veterinary Medical Association, 2007. U.S. Pet Ownership and Demographics Sourcebook. Schaumburg, IL: American Veterinary Medical Association.
  63. Poss JE, Bader JO, , 2007. Attitudes toward companion animals among Hispanic residents of a Texas border community. J Appl Anim Welf Sci 10: 243253.[Crossref]
  64. U.S. Census Bureau, 2014. State and County Quickfacts: Hidalgo County, Cameron County, Starr County, Willacy County, TX. Available at: http://quickfacts.census.gov. Accessed September 14, 2016.
  65. Jimenez-Coello M, Poot-Cob M, Ortega-Pacheco A, Guzman-Marin E, Ramos-Ligonio A, Sauri-Arceo CH, Acosta-Viana KY, , 2008. American trypanosomiasis in dogs from an urban and rural area of Yucatan, Mexico. Vector Borne Zoonotic Dis 8: 755761.[Crossref]
  66. Afonso AM, Ebell MH, Tarleton RL, , 2012. A systematic review of high quality diagnostic tests for Chagas disease. PLoS Negl Trop Dis 6: e1881.[Crossref]
  67. Tarleton RL, Gurtler RE, Urbina JA, Ramsey J, Viotti R, , 2014. Chagas disease and the London declaration on neglected tropical diseases. PLoS Negl Trop Dis 8: 813.[Crossref]
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  • Received : 05 Oct 2016
  • Accepted : 31 Dec 2016

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